Liver Protection Effect of Steamed Codonopsis lanceolata on Alcohol-induced Liver Injury in Mice and its Main Components by LC/MS Analysis

2019 ◽  
Vol 15 (3) ◽  
pp. 394-402 ◽  
Author(s):  
Shu-quan Xin ◽  
Zi Wang ◽  
Wei-Nan Hao ◽  
Xiao-Tong Yan ◽  
Qi Xu ◽  
...  
Keyword(s):  
Liver Injury ◽  
Codonopsis Lanceolata ◽  
Protection Effect ◽  
Liver Protection ◽  
Ms Analysis ◽  
Main Components

scholarly journals An Integrated Strategy for Rapid Discovery and Identification of Quality Markers in Gardenia Fructus Using an Omics Discrimination-Grey Correlation-Biological Verification Method

2021 ◽  
Vol 12 ◽  
Author(s):  
Rong Dong ◽  
Qingping Tian ◽  
Yongping Shi ◽  
Shanjun Chen ◽  
Yougang Zhang ◽  
...  
Keyword(s):  
Liver Injury ◽  
Evaluation System ◽  
Multiple Infection ◽  
Active Components ◽  
Grey Correlation ◽  
Liver Protection ◽  
Quality Markers ◽  
Anti Inflammatory ◽  
Highly Correlated ◽  
Tof Ms

Background: Gardenia Fructus (GF), a traditional Chinese medicine of Gardenia Ellis in Rubiaceae family, has the potential to clear heat and purge fire and has been widely used to treat multiple infection-related diseases. However, the quality markers (Q-Markers) of GF have not been revealed comprehensively.Methods: In this experiment, the transgenic zebrafish lines, Tg (l-fabp:EGFP) and Tg (lyz:EGFP), were used to evaluate two main kinds of traditional efficacies of GF, hepatoprotective and anti-inflammatory effects. All the GF samples from different production areas were tested their anti-liver injury and anti-inflammantory activities. High-performance liquid chromatography-quadrupole time-of-flight mass spectrometry method (HPLC-Q-TOF/MS) was employed for herbal metabonomic analysis of GF samples. Gray correlation analysis (GCA) was utilized to screen out the components closely associated with the activities. Finally, the zebrafish model was applied to verify the bioactivity of the crucial components to determine the Q-Markers of GF.Results: The zebrafish models were established by inducing with hydrogen peroxide or copper sulfate and applied to quickly evaluate the hepatoprotective effect and inflammation of GF samples. 27 potentially active components for liver protection and 21 potentially active components with anti-inflammatory properties were identified by herbal metabolomic analysis based on HPLC-Q-TOF/MS. The GCA result showed that five of the 27 components were highly correlated with liver protection, 15 of the 21 components were highly correlated with anti-inflammatory activity. Among them, geniposide and crocin-1 were confirmed their bioactivities on zebrafish experiment to be responsible for the protective effects of GF against liver injury, and genipin-1-β-D-gentiobioside, quinic acid, gardenoside, d-glucuronic acid, l-malic acid, mannitol, rutin, and chlorogenic acid were confirmed to be responsible for the anti-inflammatory effects. Finally, according to the screening principles of Q-Markers, genipin-1-β-D-gentiobioside, geniposide, and gardenoside were preliminarily identified to be the Q-Markers of GF.Conclusion: This study established an effective research strategy of “Omics Discrimination-Grey Correlation-Biological Verification,” which enabled the rapid identification of key pharmacological components of GF. These markers have provided a scientific basis for constructing a modern quality evaluation system for GF.

scholarly journals GC/MS Analysis and Antimicrobial Activity of the Essential Oil of Fresh Leaves of Eucalytus Globulus, and Leaves and Stems of Smyrnium Olusatrum from Constantine (Algeria)

2010 ◽  
Vol 5 (10) ◽  
pp. 1934578X1000501 ◽  
Author(s):  
Habiba Daroui-Mokaddem ◽  
Ahmed Kabouche ◽  
Mabrouka Bouacha ◽  
Boudjemaa Soumati ◽  
Aida El-Azzouny ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Eucalyptus Globulus ◽  
Bacterial Strains ◽  
Ms Analysis ◽  
Wide Range ◽  
Main Components ◽  
Type Apparatus

The essential oils of fresh leaves of Eucalyptus globulus L. (Myrtaceae), and leaves and stems of Smyrnium olusatrum L. (Apiaceae), obtained by hydrodistillation in a Clevenger-type apparatus, were analyzed by GC/MS. Twenty compounds were characterized, representing 98.3% of the essential oil of E. globulus, with 1,8-cineole (48.6%), globulol (10.9%), trans-pinocarveol (10.7%), and α-terpineol (6.6%) as main components, while thirty-three compounds were characterized, representing 94.3% of the essential oil of S. olusatrum, with sabinene (27.1%), curzerene (13.7%), methyl-1-benzyl-2-oxocyclooctane carboxylate (12.3%), α-pinene (7.2%), cryptone (7.1%) and β-pinene (5.7%) as the major components. The essential oils of E. globulus and S. olusatrum were tested against a wide range of fungal and bacterial strains. Both oils showed significant antimicrobial activity.

The preventive effects of OK432 on endotoxin-induced liver injury: Liver protection by the modulation of hepatic macrophage function

Surgery Today ◽  
1996 ◽  
Vol 26 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Shinichi Fujita ◽  
Shigeki Arii ◽  
Kazunobu Monden ◽  
Satoshi Ishiguro ◽  
Toshio Nakamura ◽  
...  
Keyword(s):  
Liver Injury ◽  
Macrophage Function ◽  
Liver Protection ◽  
Preventive Effects ◽  
Hepatic Macrophage

Py-GC/MS Analysis of Bioactive Components of 450°C Pyrolyzate from Ethanol Extractives of Oil-Tea Cake

2011 ◽  
Vol 480-481 ◽  
pp. 513-518
Author(s):  
Qi Mei Liu ◽  
Wan Xi Peng
Keyword(s):  
Phosphonic Acid ◽  
Value Added ◽  
Bioactive Components ◽  
Cyclobutanedicarboxylic Acid ◽  
Ms Analysis ◽  
Cosmetic Industry ◽  
Analytical Result ◽  
Main Components

450°C-based pyrolysis- GC/MS technology was used to analyze the bioactive components of ethanol extractives of oil-tea cake. The analytical result showed that 38 peaks were obtained from the 450°C-based pyrolyzate of ethanol extractives of oil-tea cake, and 37 compounds representing 97.81 % of the total areas were identified (Table 1). The analytical result showed that the main components of ethanol extractives of oil-tea cake by 450°C-based pyrolysis- GC/MS were as: Benzene, 1,2,3-Trimethoxy-5-Methyl- (21.56%), Phenol, 3,4-Dimethoxy- (16.31%), 3',5'-Dimethoxyacetophenone (14.37%), 1,2-Ethanediamine, N-Ethyl- (6.62%), Indole (5.39%), Mequinol (4.13%), Phosphonic Acid, (P-Hydroxyphenyl)- (2.97%), 1,2-Cyclobutanedicarboxylic Acid, Cis- (1.67%), Bicyclo 3.1.0 Hexan-3-One (1.38%), Benzene, 1,4-Dimethoxy-2,3,5,6- Tetramethyl- (1.35%), 2h-Azepin-2-One, Hexahydro-1-(2-Propenyl)- (1.15), etc. The results of function analyses showed that the 450°C-based pyrolyzate of ethanol extractives of oil-tea cake can be used as rare natural medicinal materials, and value-added materials of spice, food and cosmetic industry.

Bovine jugular vein valved xenograft for extracardiac total cavo‐pulmonary connection: The risk of thrombosis and the potential liver protection effect

2020 ◽  
Vol 35 (4) ◽  
pp. 845-853 ◽  
Author(s):  
Mohamed F. Ismail ◽  
Ahmed F. Elmahrouk ◽  
Amr A. Arafat ◽  
Tamer E. Hamouda ◽  
Azzahra Edrees ◽  
...  
Keyword(s):  
Jugular Vein ◽  
Protection Effect ◽  
Liver Protection ◽  
Bovine Jugular Vein

Essential Oil of Alfavaca, Ocimum gratissimum, from Brazilian Amazon

1990 ◽  
Vol 45 (9-10) ◽  
pp. 1073-1076 ◽  
Author(s):  
Otto Vostrowsky ◽  
Wolfgang Garbe ◽  
Hans-Jürgen Bestmann ◽  
José Guilherme S. Maia
Keyword(s):  
Essential Oil ◽  
Brazilian Amazon ◽  
Methyl Eugenol ◽  
Ocimum Gratissimum ◽  
Steam Distillate ◽  
Ms Analysis ◽  
Sesquiterpene Hydrocarbons ◽  
Main Components

Abstract13CNMR , GC and GC/MS analysis of the steam distillate from leaves and stems of alfavaca, Ocimum gratissimum, from the Brazilian Amazon resulted in the identification of methyl eugenol (46.83%) and eugenol (19.26%) as the main components, and another 34 compounds, mainly monoterpene and sesquiterpene hydrocarbons

Mo1050 The Liver Protection Effect of L-Carnitine Administration After TACE on Intermediate-Stage Hepatocellular Carcinoma Patients

2014 ◽  
Vol 146 (5) ◽  
pp. S-996
Author(s):  
Yasuhiro Tsuda ◽  
Hideko Ohama ◽  
Keisuke Yokohama ◽  
Tetsuya Sujishi ◽  
Yusuke Tsuchimoto ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Intermediate Stage ◽  
Protection Effect ◽  
Liver Protection
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