Protection Effect and Mechanism of Huangshan Maofeng Tea Polyphenols Extracts on CCl4-Induced Liver Injury

Green tea polyphenols (GTPs) have been shown to exhibit diverse beneficial effects against a variety of diseases. Acetaminophen (APAP) overdose is one of the most frequent causes of drug-induced liver injury. In the current study, we aimed to investigate the protective effect of GTP on APAP-induced liver injury in mice and the underlying mechanisms involved. Male C57BL/6J mice were treated orally with different doses of GTP (37.5, 75, or 150 mg/kg) 4 h after APAP overdose (400 mg/kg) and continuously given every 8 h until sacrificed at 4, 12, 20, and 48 h after the first treatment of GTP. Survival rate and histological and biochemical assessments were performed to evaluate the APAP-induced liver injury. Protein expression of multiple drug metabolizing enzymes and transporters was measured to demonstrate the possible mechanisms involved. Our results revealed that administration of different doses of GTP significantly alleviated APAP-induced liver injury by improving the survival rate, hepatocellular necrosis, and ALT/AST/GSH levels after APAP overdose (400 mg/kg). The protein expression of APAP-induced drug transporters and metabolizing enzymes was mostly induced by GTP treatment, which was followed by reduction in drug transporters at the later time points. The current study collectively demonstrated that GTP protects against APAP-induced liver injury, possibly through regulating drug metabolizing enzymes and transporters after APAP overdose.

Download Full-text

The potential roles of nitric oxide in carbon tetrachloride induced liver injury of mice and the protective effects of green tea polyphenols

10.5353/th_b3124142 ◽

1999 ◽

Author(s):

Wen Zhu

Keyword(s):

Nitric Oxide ◽

Carbon Tetrachloride ◽

Liver Injury ◽

Green Tea ◽

Tea Polyphenols ◽

Green Tea Polyphenols ◽

Protective Effects

Download Full-text

Tea polyphenols protect mice from acute ethanol-Induced liver injury by modulating the gut microbiota and short-chain fatty acids

Journal of Functional Foods ◽

10.1016/j.jff.2021.104865 ◽

2021 ◽

Vol 87 ◽

pp. 104865

Author(s):

Yue Sun ◽

Kai Kang ◽

Yi-Ling Li ◽

Li-Xuan Sang ◽

Bing Chang

Keyword(s):

Fatty Acids ◽

Liver Injury ◽

Gut Microbiota ◽

Short Chain Fatty Acids ◽

Tea Polyphenols ◽

Short Chain ◽

Acute Ethanol ◽

Chain Fatty Acids

Download Full-text

Liver Injury Induced by Carbon Tetrachloride in Mice Is Prevented by the Antioxidant Capacity of Anji White Tea Polyphenols

Antioxidants ◽

10.3390/antiox8030064 ◽

2019 ◽

Vol 8 (3) ◽

pp. 64 ◽

Cited By ~ 17

Author(s):

Ranran Wang ◽

Zhiqing Yang ◽

Jing Zhang ◽

Jianfei Mu ◽

Xianrong Zhou ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Liver Injury ◽

Antioxidant Capacity ◽

Liver Tissue ◽

Quantitative Polymerase Chain Reaction ◽

Interleukin 1 ◽

Antioxidant Properties ◽

Tea Polyphenols ◽

Nuclear Factor ◽

White Tea

Anji white tea is a unique variety of green tea that is rich in polyphenols. In this study, the effect of Anji white tea polyphenols (AJWTP) on the prevention of carbon tetrachloride (CCl4)-induced liver injury through its antioxidant properties was studied. Biochemical and molecular biology methods were used to analyze the serum and liver tissue of mice. The antioxidant capacity and liver injury preventive effect of AJWTP were determined, and the mechanism was elaborated. The results showed that AJWTP decreased the serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride (TG), and total cholesterol (TC) in mice with liver injury, it increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the serum and liver tissue of mice with liver injury, and it also decreased the amount of malondialdehyde (MDA). Further quantitative polymerase chain reaction (qPCR) results showed that AJWTP upregulated the mRNA expression of Cu/Zn-SOD, Mn-SOD, catalase (CAT), and nuclear factor of kappa light polypeptide gene enhancer in B-cell inhibitor alpha (IκB-α) and downregulated the expression of nuclear factor κ-light-chain-enhancer of activated B-cells (NF-κB), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), interleukin-1 beta (IL-1β), and tumor necrosis factor alpha (TNF-α) in the liver tissue of mice with liver injury. Therefore, AJWTP produces sufficient antioxidant action to prevent liver injury, and the effect increases with the increase in AJWTP concentration. The effect of 200 mg/kg AJWTP was similar to that of the same concentration of the drug (silymarin) used for the treatment of liver injury. This indicates excellent potential for the development and utilization of AJWTP because it is an active substance with excellent antioxidant effects and can prevent liver injury.

Download Full-text