scholarly journals The Effect of an Attenuated Rabies Virus SRV9 on Suckling Mouse Growth After Intracerebral Inoculation

2011 ◽  
Vol 10 (3) ◽  
pp. 322-326 ◽  
Author(s):  
Xiaohu Wang ◽  
Yuzhu Jin ◽  
Chenglong Sun ◽  
Shoufeng Zhang ◽  
Ziguo Yuan ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Suckling Mouse ◽  
Intracerebral Inoculation

scholarly journals On the evolution of fixed strains of rabies virus

1938 ◽  
Vol 38 (2) ◽  
pp. 180-184 ◽  
Author(s):  
Pierre Lépine
Keyword(s):  
Rabies Virus ◽  
Intracerebral Inoculation ◽  
Variable Period ◽  
Repeated Passage

We know, since the time of Pasteur, that repeated passage of rabies street virus from rabbit to rabbit after intracerebral inoculation, leads to a modification of its effect and the appearance of mutative characters. This takes place at a variable period of time depending upon the strain employed. The irreversibility and the permanence of these characters has led to the name of “fixed virus” once the street virus has become adapted for the rabbit.

scholarly journals Experiments with the soluble antigen of rabies in suckling mouse brains

1957 ◽  
Vol 55 (3) ◽  
pp. 361-373 ◽  
Author(s):  
M. van den Ende ◽  
A. Polson ◽  
G. S. Turner
Keyword(s):  
Rabies Virus ◽  
Neutralizing Antibody ◽  
Financial Assistance ◽  
Experimental Results ◽  
Soluble Antigen ◽  
Infective Virus ◽  
Suckling Mouse ◽  
High Concentration ◽  
Live Virus

A study has been made of the properties of soluble antigen in the brains of infant mice infected intracerebrally with the Flury strain of rabies virus.Soluble antigen is produced at the same time as infective virus, and reaches a high concentration in a period of 2–3 days.It can be partially purified by precipitation at pH 4·3. It is partially resistant to the action of trypsin, RNAse and DNAse. It is relatively stable at pH 6–10.Experimental results suggest that the soluble antigen remains antigenically active after heating at 56° C. and treatment with 0·5% phenol or 0·35% formal-dehyde, but that such heating markedly reduces the ability to stimulate formation of neutralizing antibody.Rabbits and mice appear to differ in the production of neutralizing antibody following immunization against soluble antigen in which residual live virus was inactivated by heat, phenol or formaldehyde.It is suggested that this difference may depend on the different susceptibility to traces of incompletely inactivated virus remaining in the immunizing antigens.The authors are grateful to Miss T. Madsen for her assistance in some aspects of this work. Dr N. Sapeika kindly made available facilities for the in vitro anaphylaxis experiments.Financial assistance was received from the Nkana-Kitwe and Chingola Poliomyelitis Research Funds.

scholarly journals Rescue of Rabies Virus from Cloned cDNA and Identification of the Pathogenicity-Related Gene: Glycoprotein Gene Is Associated with Virulence for Adult Mice

2001 ◽  
Vol 75 (19) ◽  
pp. 9121-9128 ◽  
Author(s):  
Naoto Ito ◽  
Mutsuyo Takayama ◽  
Kentaro Yamada ◽  
Makoto Sugiyama ◽  
Nobuyuki Minamoto
Keyword(s):  
Rabies Virus ◽  
Reverse Genetics ◽  
Biological Properties ◽  
Open Reading Frame ◽  
Chimeric Virus ◽  
Viral Gene ◽  
Reading Frame ◽  
Intracerebral Inoculation ◽  
Glycoprotein Gene ◽  
Adult Mice

ABSTRACT In order to identify the viral gene related to the pathogenicity of rabies virus, we tried to establish a reverse genetics system of the attenuated RC-HL strain, which causes nonlethal infection in adult mice after intracerebral inoculation. A full-length genome plasmid encoding the complete antigenomic cDNA of the RC-HL strain and helper plasmids containing cDNAs of the complete open reading frame of the N, P, and L genes, respectively, were constructed. After transfection of these plasmids into BHK-21 cells infected with the T7 RNA polymerase-expressing vaccinia virus, infectious rabies virus with almost the same biological properties as those of the wild-type RC-HL strain was rescued. Using this reverse genetics system of the RC-HL strain, we generated a chimeric virus with the open reading frame of the glycoprotein gene from the parent Nishigahara strain, which kills adult mice after intracerebral inoculation, in the background of the RC-HL genome. Since the chimeric virus killed adult mice following intracerebral inoculation, it became evident that the open reading frame of the glycoprotein gene is related to the pathogenicity of the Nishigahara strain for adult mice.

scholarly journals Lesions of the Central Nervous System Induced by Intracerebral Inoculation of BALB/c Mice with Rabies Virus (CVS-11)

2010 ◽  
Vol 72 (8) ◽  
pp. 1011-1016 ◽  
Author(s):  
Daisuke KOJIMA ◽  
Chun-Ho PARK ◽  
Shintarou TSUJIKAWA ◽  
Keiko KOHARA ◽  
Hitoshi HATAI ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Rabies Virus ◽  
Intracerebral Inoculation ◽  
The Central Nervous System

scholarly journals AN ELISA SUITABLE FOR THE DETECTION OF RABIES VIRUS ANTIBODIES IN SERUM SAMPLES FROM HUMAN VACCINATED WITH EITHER CELL-CULTURE VACCINE OR SUCKLING-MOUSE-BRAIN VACCINE

1999 ◽  
Vol 41 (1) ◽  
pp. 39-43 ◽  
Author(s):  
Adriana Souza de Toledo PIZA ◽  
Jair Lício Ferreira SANTOS ◽  
Luciana Botelho CHAVES ◽  
Carlos Roberto ZANETTI
Keyword(s):  
Cell Culture ◽  
Rabies Virus ◽  
Mouse Brain ◽  
Neutralization Test ◽  
Protein A ◽  
Staphylococcal Protein A ◽  
Suckling Mouse ◽  
Serum Samples ◽  
Igg Antibody ◽  
Human Sera

An indirect ELISA for determination of post-vaccination rabies antibody was applied. Purified rabies virus was used as antigen to coat plates, and staphylococcal protein A linked with horseradish peroxidase was used for detecting IgG antibody in human sera. Sera from humans, vaccinated with cell-culture vaccine or suckling-mouse-brain vaccine, were examined. ELISA results were compared to those obtained from the virus neutralization test. The mean and standard deviation of OD were determined for 126 negative sera (pre-vaccination) and for 73 sera from vaccinated persons showing antibody titers lower than 0.5 IU/ml. Results were defined as ELISA -positive, -negative or -doubtful. Establishment of a doubtful region reduced the number of sera otherwise classified as positive (false-positive sera). In this way, the sensitivity, specificity and agreement values were respectively 87.5%, 92.4% and 88.5%. No significant differences were observed in these values when the group vaccinated with cell-culture vaccine and the group vaccinated with suckling-mouse-brain vaccine were compared. It was shown that much of the disagreement between the values obtained by neutralization test and ELISA occurred in sera obtained at the beginning of the immunization process, and was probably due to the presence of IgM in the serum samples, detected only by the former test. This ELISA method can be used as a screening test in rabies laboratories regardless of the kind of vaccine used for immunization.

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