scholarly journals The Pathogenesis of Salmonella enterica Serovar Enteritidis in Subcutaneously Infected Pigeon: A Quantitative Time-Course Study Using Real-Time PCR

2011 ◽  
Vol 10 (7) ◽  
pp. 909-913 ◽  
Author(s):  
Guang-Zhi He ◽  
Wei-Yi Tian ◽  
Ning Qian ◽  
Shu-Xuan Deng
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Salmonella Enterica ◽  
Time Course ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Time Course Study

The population of a high-virulence strain of Salmonella enterica serovar Enteritidis in subcutaneously infected partridge: a quantitative time-course study using real-time PCR

2011 ◽  
Vol 35 (7) ◽  
pp. 439-445
Author(s):  
Guang-Zhi He ◽  
Wei-Yi Tian ◽  
Ning Qian ◽  
Shu-Xuan Deng ◽  
Chuan-Wei An ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Salmonella Enterica ◽  
Time Course ◽  
Salmonella Enterica Serovar Enteritidis ◽  
High Virulence ◽  
Time Course Study

Replication kinetics of Salmonella enteritidis in internal organs of ducklings after oral challenge: a quantitative time-course study using real-time PCR

2008 ◽  
Vol 33 (3) ◽  
pp. 273-280 ◽  
Author(s):  
S. X. Deng ◽  
A. C. Cheng ◽  
M. S. Wang ◽  
X. R. Li ◽  
B. Yan
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Salmonella Enteritidis ◽  
Time Course ◽  
Oral Challenge ◽  
Internal Organs ◽  
Time Course Study ◽  
Kinetics Of

scholarly journals A multiplex real-time PCR assay targeting virulence and resistance genes in Salmonella enterica serotype Typhimurium

2011 ◽  
Vol 11 (1) ◽  
pp. 151 ◽  
Author(s):  
Marie Bugarel ◽  
Sophie A Granier ◽  
François-Xavier Weill ◽  
Patrick Fach ◽  
Anne Brisabois
Keyword(s):  
Real Time ◽  
Resistance Genes ◽  
Real Time Pcr ◽  
Salmonella Enterica ◽  
Pcr Assay

scholarly journals Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus

2015 ◽  
Vol 54 (1) ◽  
pp. 49-58 ◽  
Author(s):  
Jie Liu ◽  
Caroline Ochieng ◽  
Steve Wiersma ◽  
Ute Ströher ◽  
Jonathan S. Towner ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Salmonella Enterica ◽  
Ebola Virus ◽  
Febrile Illness ◽  
Fever Virus ◽  
Outbreak Investigation ◽  
Acute Febrile Illness ◽  
Content Type ◽  
Taqman Array

Acute febrile illness (AFI) is associated with substantial morbidity and mortality worldwide, yet an etiologic agent is often not identified. Convalescent-phase serology is impractical, blood culture is slow, and many pathogens are fastidious or impossible to cultivate. We developed a real-time PCR-based TaqMan array card (TAC) that can test six to eight samples within 2.5 h from sample to results and can simultaneously detect 26 AFI-associated organisms, including 15 viruses (chikungunya, Crimean-Congo hemorrhagic fever [CCHF] virus, dengue, Ebola virus, Bundibugyo virus, Sudan virus, hantaviruses [Hantaan and Seoul], hepatitis E, Marburg, Nipah virus, o'nyong-nyong virus, Rift Valley fever virus, West Nile virus, and yellow fever virus), 8 bacteria (Bartonellaspp.,Brucellaspp.,Coxiella burnetii,Leptospiraspp.,Rickettsiaspp.,Salmonella entericaandSalmonella entericaserovar Typhi, andYersinia pestis), and 3 protozoa (Leishmaniaspp.,Plasmodiumspp., andTrypanosoma brucei). Two extrinsic controls (phocine herpesvirus 1 and bacteriophage MS2) were included to ensure extraction and amplification efficiency. Analytical validation was performed on spiked specimens for linearity, intra-assay precision, interassay precision, limit of detection, and specificity. The performance of the card on clinical specimens was evaluated with 1,050 blood samples by comparison to the individual real-time PCR assays, and the TAC exhibited an overall 88% (278/315; 95% confidence interval [CI], 84% to 92%) sensitivity and a 99% (5,261/5,326, 98% to 99%) specificity. This TaqMan array card can be used in field settings as a rapid screen for outbreak investigation or for the surveillance of pathogens, including Ebola virus.

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