scholarly journals Monitoring the Effect of Insecticide Selection on Culex pipiens (Diptera: Culicidae) Larval Susceptibility to Malathion and Lambda-Cyhalothrin

2013 ◽  
Vol 11 (1) ◽  
pp. 14-24 ◽  
Author(s):  
El-Sayed A. El-Sheikh ◽  
Mohamed-Bassem A. Ashour ◽  
Mohamed M. Aamir ◽  
Mohamed M. Gamal
Author(s):  
Sara Rahimi ◽  
Hassan Vatandoost ◽  
Mohammad Reza Abai ◽  
Ahmad Raeisi ◽  
Ahmad Ali Hanafi-Bojd

Background: West Nile virus (WNV) can cause a fatal disease in humans and it is mainly transmitted to people through the bites of infected mosquitoes. Vector control using insecticides is a very important goal. Study of Culex pipiens re­sistance towards several insecticides in the city of Tehran, Iran was evaluated. Methods: Adult females reared from field-caught larvae from southern part of Tehran and lab strain reared in the insec­tary of Tehran University of Medical Science were determined for resistant status by exposing to 4% DDT, 0.1% bendi­ocarb, 0.1% propoxur, 1% fenitrothion, 0.05% deltamethrin, 0.75% permethrin, 0.05% lambda-cyhalothrin, 0.5% etofenprox, 5% malathion and 0.15% cyfluthrin papers using the standard WHO susceptibility tests.   Results: Results clearly showed resistance development of Cx. pipiens against tested insecticides. Mortalities of Cx. pipiens were less than 90% with high resistance, low knock down rate and knock down time (50%) observed against insec­ti­cides. DDT and Malathion showed the most and least lethal time (LT50) values for the field strain. The results of the knock­down test showed that DDT and deltamethrin had the most and least knockdown times (50%) for the field strain, re­spec­tively, while DDT and lambda-cyhalothrin had the most and least knockdown times (50%) for the lab strain, respectively. Conclusion: Resistance to mentioned insecticides in Cx. pipiens is widely distributed in southern part of Tehran. Regu­lar implementation of susceptibility test in Cx. pipiens mosquitoes will help local public health authorities to develop new and better control strategies.  


Author(s):  
Onder Ser ◽  
Huseyin Cetin

Background: Culex pipiens L. (Diptera: Culicidae) is an important vector of several pathogens. This mosquito is widely distributed throughout the world. We aimed to determine the susceptibility levels of Cx. pipiens populations to some synthetic pyrethroid insecticides in Antalya, Turkey. Methods: The immature stages of mosquitoes were collected from eight locations in Alanya, Döşemealtı, Kemer, Kumluca, and Manavgat districts of Antalya between Apr and Oct of 2017. Adult susceptibility tests were carried out according to a modified version of the Centers for Disease Control and Prevention bottle bioassay. In the tests, the World Health Organization recommended diagnostic doses; permethrin (0.75%), etofenprox (0.5%), deltamethrin (0.05%) and lambda-cyhalothrin (0.05%) were used. Results: As a result of the susceptibility tests, deltamethrin was the least effective insecticide and it caused 58.78–97.56% mortalities on Cx. pipiens populations while permethrin was the most effective substance that caused 100% mortality on all populations. While all of the tested populations were found susceptible to permethrin, and possible resistant or resistant to deltamethrin. Etofenprox and lambda-cyhalothrin led to 91.54–100% and 93.1–100% mortali­ties, respectively. Conclusion: The possible resistance or resistance to deltamethrin in all the areas is caused by the widespread use of this chemical against pests in agriculture and public health applications for long-term. Moreover, a concordance was found between resistance levels and the intensity of pesticide application in agriculture and public health, and organic and chemical pollution levels in the sampled habitats.


Author(s):  
Brendan Clifford

An ultrastructural investigation of the Malpighian tubules of the fourth instar larva of Culex pipiens was undertaken as part of a continuing study of the fine structure of transport epithelia.Each of the five Malpighian tubules was found to be morphologically identical and regionally undifferentiated. Two distinct cell types, the primary and stellate, were found intermingled along the length of each tubule. The ultrastructure of the stellate cell was previously described in the Malpighian tubule of the blowfly, Calliphora erythrocephala by Berridge and Oschman.The basal plasma membrane of the primary cell is extremely irregular, giving rise to a complex interconnecting network of basal channels. The compartments of cytoplasm entrapped within this system of basal infoldings contain mitochondria, free ribosomes, and small amounts of rough endoplasmic reticulum. The mitochondria are distinctive in that the cristae run parallel to the long axis of the organelle.


Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
SA Abdelgaleil ◽  
T Suganuma ◽  
K Kitahara ◽  
M Fujii
Keyword(s):  

2020 ◽  
Vol 14 (1) ◽  
pp. 81-88
Author(s):  
Fedor I. Vasilevich ◽  
Anna M. Nikanorova

The purpose of the research is development of preventive measures against zooanthroponoze vector-borne diseases spread by parasitic arthropods in the Kaluga Region. Materials and methods. The subject of the research was Ixodidae, mosquitoes, and small mammals inhabiting the Kaluga Region. The census of parasitic arthropods was carried out on the territory of all districts of the Kaluga Region and the city of Kaluga. Open natural habitat and human settlements were investigated. Weather conditions from 2013 to 2018 were also taken into account. For the purposes of the study, we used standard methods for capturing and counting arthropods and mouse-like rodents. In order to obtain mathematical models of small mammal populations, a full factorial experiment was conducted using the collected statistical data. In-process testing of the drug based on s-fenvalerate and piperonyl butoxide were carried out under the conditions of the agricultural collective farm “Niva” of the Kozelsky District, the Kaluga Region, and LLC “Angus Center of Genetics” of the Babyninsky District, the Kaluga Region. Results and discussion. In the Kaluga Region, two species of ixodic ticks are found, namely, Ixodes ricinus and Dermacentor reticulatus, which have two activity peaks. Mosquito may have 3-4 generations in a year in the Kaluga region. The most common mosquito species in the Kaluga Region are Aedes communis, Ae. (Och.) togoi and Ae. (Och.) diantaeus, Culex pipiens Culex Linnaeus, 1758 (Diptera, Culicidae) (Culex pipiens): Cx. pipiens f. pipiens L. (non-autogenic form) and Cx. p. f. molestus Fors. (autogenic form), which interbreed, and reproductively isolated in the Region. The developed mathematical models make it possible to quantify the risks of outbreaks of zooanthroponoze vector-borne diseases without the cost of field research, and allow for rational, timely and effective preventive measures. Medications based on s-fenvalerate and piperonyl butoxide and based on cyfluthrin showed high insecto-acaricidal efficacy and safety.


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