Antifungal Potential and Chemical Composition of Essential Oils Extracted From Artemisia herba-alba and Salvia lavandulifolia Plants

The objective of this research was to analyze the chemical composition, antimicrobial and cytotoxic activity of Heracleum pyrenaicum subsp. pollinianum (Bertol.) F. Pedrotti & Pignatti (HPP) and H. orphanidis Boiss. (HO) essential oils. The composition of the oils was analyzed by GC and GC-MS. β-Pinene (35.1%) was the most abundant compound in HPP root oil, while ( Z)-falcarinol (80.0%) dominated in HO root oil. ( E)-Nerolidol (28.5%) was the main constituent in HPP leaf oil. HPP fruit oil, as well as HO leaf and fruit oils mainly contained aliphatic esters, mostly octyl acetate (50.5–84.5%). Antimicrobial screening was performed by microdilution method against eight bacterial and eight fungal strains. The strongest antibacterial activity was shown by both root oils (MICs 0.02–0.60 mg/mL and MBCs 0.04–2.50 mg/mL for HPP, and MICs 0.02–1.25 mg/mL and MBCs 0.04–2.50 mg/mL for HO), while the best antifungal potential was exhibited by HPP fruit oil (MICs 0.30–0.60 mg/mL and MFCs 0.60–1.25 mg/mL) and HO leaf oil (MICs 0.15–0.63 mg/mL and MFCs 0.30–1.25 mg/mL). The tested root and fruit oils exhibited strong cytotoxic effect, which was determined by MTT test against HeLa (IC50 7.53–21.07 μg/mL) and LS174 (IC50 24.16–58.86 μg/mL) cell lines.

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Chemical composition and antifungal potential of essential oils from different aerial parts of Protium ovatum Engl

Australian Journal of Crop Science ◽

10.21475/ajcs.21.15.04.p2915 ◽

2021 ◽

pp. 570-576

Author(s):

Wendel C. de Sousa ◽

Josemar G. Oliveira Filho ◽

Cassia C. F. Alves ◽

Moacir R. Forim ◽

Cristiane de M. Cazal

Keyword(s):

Chemical Composition ◽

Antifungal Activity ◽

Essential Oil ◽

Essential Oils ◽

Antimicrobial Agents ◽

Ripe Fruit ◽

Aerial Parts ◽

Potential Source ◽

Antifungal Potential ◽

Germacrene D

Protium ovatum is a Brazilian endemic species widely distributed between the Cerrado and Amazon biomes. Here, we evaluate the chemical composition of the essential oils (EO) of different shoot organs from P. ovatum including stems, petioles, leaves, flowers, ripe and unripe fruits, and investigate their antifungal potential against Sclerotinea sclerotiorum. The EO were obtained by hydrodistillation in a Clevenger-type apparatus and analysed by GC-MS, followed by PCA analysis. The antifungal activity was performed by agar diffusion. Fruits had the highest essential oil contents among the shoot parts. The constituents were found varyingin the different organs: α-Pinene (0.80-18.3%), β-Pinene (0.58-5.17%), Myrcene (0.52-27.3%), Limonene (3.15-59.7%), Caryophyllene E (3.67-16.4%), Germacrene D (6.34-27.4%), and δ-Cadinene (2.29-7.63%). The essential oil from ripe fruit showed the strongest antifungal activity, with the highest Inhibition of Mycelial Growth (IMG) (50.11%) at the lowest concentration assayed (18.75μg.mL-1). This is the first report on the chemical composition of the essential oils from stems, petioles, flowers, and ripe fruits of P. ovatum and their antifungal activity against S. sclerotiorum, making it a potential source of antimicrobial agents

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