Fingerprinting of Cotton (Gossypium Spp.) Hybrids and their Parental Lines Using Microsatellite Markers and their Utilization in Genetic Purity Assessment

DNA fingerprinting and genetic diversity of four Bangladesh Rice Research Institute (BRRI) hybrid varieties and their parental lines were carried out. A total of 73 microsatellite markers were tested for screening the genotypes. Among the 73 amplified products, 37% had polymorphic bands showing 81 alleles. The number of alleles per locus ranged from two (RM10) to eight (RM327), where average allele number was 4.333. The Polymorphism Information Contents (PIC) lied between 0.337 (RM10) and 0.852 (RM327). RM327 was the most robust marker providing the highest PIC value (0.852). Pair-wise genetic dissimilarity coefficient interaction showed that BRRI hybrids two was the most genetically distant from each other whereas BRRI hybrids one, three, four and their respective parents were very close. Cluster analysis based on Dices similarity coefficient UPGMA system grouped BRRI hybrid and their parental lines into four major clusters at 0.41 cut off similarity coefficient. Four BRRI hybrid varieties grouped into four distinct clusters along with their component lines indicating their genetic closeness. Key words: Hybrid rice, Diversity analysis, Microsatellite markers, DNA fingerprinting D. O. I. 10.3329/ptcb.v21i2.10242 Plant Tissue Cult. & Biotech. 21(2): 189-198, 2011 (December)

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DNA Fingerprinting for Identification of Rice Varieties and Seed Genetic Purity Assessment

Agricultural Research ◽

10.1007/s40003-018-0324-8 ◽

2018 ◽

Vol 7 (4) ◽

pp. 379-390 ◽

Cited By ~ 4

Author(s):

Vanisri Satturu ◽

Durga Rani ◽

Swathi Gattu ◽

Jamal Md ◽

Sreedhar Mulinti ◽

...

Keyword(s):

Dna Fingerprinting ◽

Genetic Purity ◽

Rice Varieties ◽

Purity Assessment

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Genetic purity certificate in seeds of hybrid maize using molecular markers

Revista Brasileira de Sementes ◽

10.1590/s0101-31222006000100024 ◽

2006 ◽

Vol 28 (1) ◽

pp. 169-175 ◽

Cited By ~ 5

Author(s):

Kalinka Carla Padovani de Carvalho Salgado ◽

das Graças Guimarães Carvalho Vieira ◽

Édila Vilela de Resende Von Pinho ◽

Cláudia Teixeira Guimarães ◽

Renzo Garcia Von Pinho ◽

...

Keyword(s):

Isocitrate Dehydrogenase ◽

Female Parent ◽

Parental Line ◽

Maize Breeding ◽

Genetic Purity ◽

Mendelian Segregation ◽

Purity Analysis ◽

Young Leaves ◽

Parental Lines ◽

Hybrid Maize

One of the main features that confer high quality to the seed is its genetic purity, in which one of the major causes of contamination is the self-pollination of the female parent. Up to date, there is no accurate and fast methods for detecting such contamination. Thus, this work was carried out to certify the genetic purity in seeds of hybrid maize using different biochemical and DNA-based markers. Two single-cross hybrids and their parental lines derived from the maize breeding program at UFLA were evaluated by isoenzymatic pattern of alcohol dehydrogenase (ADH), esterase (EST), acid phosphatase (ACP), glutamate-oxaloacetate transaminase (GOT), malate dehydrogenase (MDH), isocitrate dehydrogenase (IDH), phosphoglucomutase (PGM), 6-phosphoglucomate dehydrogenase (PGDH), catalase (CAT) and ß-glucosidade (ßGLU) and by microsatellites markers. The enzymatic systems that were able to distinguish the hybrids from their parental line were the catalase, the isocitrate dehydrogenase and the esterase. The esterase showed a Mendelian segregation pattern for UFLA 8/3 hybrid, that enables a safer genetic purity certificate. Microsatellites were able to differentiate the hybrid lines and the respective parental lines. Moreover, this technique was fast, precise and without environment effects. For microsatellites, the amplification pattern was identical when young leaves or seeds were used as DNA source. The possibility of using seeds as DNA source would accelerate and facilitate the role process of the genetic purity analysis.

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Genetic purity and patterns of relationships among tropical highland adapted quality protein and normal maize inbred lines using microsatellite markers

Euphytica ◽

10.1007/s10681-014-1332-9 ◽

2014 ◽

Vol 204 (1) ◽

pp. 49-61 ◽

Cited By ~ 7

Author(s):

Demissew Abakemal ◽

Shimelis Hussein ◽

John Derera ◽

Kassa Semagn

Keyword(s):

Microsatellite Markers ◽

Inbred Lines ◽

Genetic Purity ◽

Maize Inbred Lines ◽

Normal Maize

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Genetic Diversity Analysis of Hybrid Rice Parental Lines and Genetic Purity Assessment of Hybrid Seeds of China

Journal of Agricultural Science ◽

10.5539/jas.v12n5p37 ◽

2020 ◽

Vol 12 (5) ◽

pp. 37

Author(s):

Haiya Cai ◽

Yuxia Lu ◽

Gang Liu ◽

Shuo Zhang ◽

Haitao Jia ◽

...

Keyword(s):

Genetic Diversity ◽

Hybrid Rice ◽

Southern China ◽

Polymorphic Information Content ◽

Diversity Analysis ◽

Japonica Rice ◽

Similarity Coefficients ◽

Genetic Purity ◽

Genetic Diversity Analysis ◽

Parental Lines

Thirty-five pairs of SSR primers were used for genetic diversity analysis and DNA fingerprinting of 31 hybrid rice core parental lines developed in central- and southern-China using one japonica rice line and three inbred rice lines as the check varieties. The average number of alleles (Na) per SSR locus was 4.02, with a range of two to eight, the effective number of alleles (Ne) was 83.16 with a mean of 2.38, ranging from 1.19 to 4.66. The polymorphic information content (PIC) ranged from 0.16 to 0.79, with an average number of 0.52. The results of the cluster analysis indicated that the check varieties viz., one japonica rice and three inbred rice, were clustered into two groups with similarity coefficients of 0.62 and 0.71 respectively indicating their relatedness. Thirty-one hybrid rice parental lines were clustered into 6 groups according to their different types, pedigrees and regions of development with similarity coefficients of approximately 0.76. The highest genetic similarity coefficient (0.94) was observed between Y58S and C815S, and the lowest (0.63) was observed between Quan9311A and Peiai64S. The purity of one hybrid rice cultivar was tested using characteristic marker and the field test, and it was demonstrated that the purities obtained using the two methods were similar. This research will be helpful for rice breeding, new cultivar registration and seed production.

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