scholarly journals Fingerprinting and genetic purity assessment of F1 barley hybrids and their salt-tolerant parental lines using nSSR molecular markers

3 Biotech ◽  
2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Mériam Ben Romdhane ◽  
Leila Riahi ◽  
Rahma Jardak ◽  
Abdelwahed Ghorbel ◽  
Nejia Zoghlami
Keyword(s):  
Molecular Markers ◽  
Salt Tolerant ◽  
Genetic Purity ◽  
Purity Assessment ◽  
Parental Lines

scholarly journals Isolation and Identification of Molecular Markers for Fingerprinting of Chilli Hybrids & its Parental Lines

2020 ◽  
Vol 11 (1) ◽  
pp. 713-716
Author(s):  
Shishir Tiwari ◽  
Shweta Sao ◽  
Antu Kurrey ◽  
Pulak Das
Keyword(s):  
Molecular Markers ◽  
Issr Marker ◽  
Genetic Purity ◽  
Isolation And Identification ◽  
Rapd Primer ◽  
Purity Analysis ◽  
Hybrid Seeds ◽  
Environmentally Sensitive ◽  
Parental Lines ◽  
Morphological Differences

Chilli (Capsicum annum) is the predominant sp., which is cultivated in both hot and sweet papers. The maintenance of the genetic purity of chilli plant is a matter of great concern for the breeders. For genetic purity analysis, between true hybrids and off-types, breeders find out morphological differences between them, but this technique is cannot be recognized easily and also costly, tedious to score, and environmentally sensitive. Alternatively,  molecular markers based genetic purity analysis can be employed. The molecular marker-based technique was thus used to overcome the conventional method drawbacks. The main objective of the study is to identify informative molecular markers (ISSR and RAPD) capable of distinguishing Chilli hybrids and their parental lines and their utilization in seed purity assessment. Five parental lines of Chilli (i.eCH10, CH12, CH530, CH709, CH734) were used for the production of 3 hybrids. Total 30 ISSR and 8 RAPD primers were selected for the study of 5 parental lines, among them 2ISSR and 1 RAPD primers produced unique fingerprinting across the hybrids. The ISSR marker UBC815 amplified alleles specific to different parental lines(CH10 & CH12) for hybrids (ACH112), The ISSR marker UBC 827, amplified alleles specific to different parental lines(CH709 & CH12) for hybrids (ACH179). Likewise, RAPD primer B20 for hybrid ACH 753 and their parental lines(CH734 & CH530). Thus, the above study showed that the aid of molecular markers is more reliable, highly efficient, and reproducible for assessing fingerprinting of Chilli commercial hybrid seeds with more accuracy.

A modified DNA extraction protocol and its utility in seed genetic purity assessment

2011 ◽  
Vol 39 (1) ◽  
pp. 236-242 ◽  
Author(s):  
S.N. Sharma ◽  
V. Kumar ◽  
G. Singh ◽  
R. Sharma
Keyword(s):  
Dna Extraction ◽  
Genetic Purity ◽  
Purity Assessment ◽  
Extraction Protocol ◽  
Modified Dna

DNA Fingerprinting for Identification of Rice Varieties and Seed Genetic Purity Assessment

2018 ◽  
Vol 7 (4) ◽  
pp. 379-390 ◽  
Author(s):  
Vanisri Satturu ◽  
Durga Rani ◽  
Swathi Gattu ◽  
Jamal Md ◽  
Sreedhar Mulinti ◽  
...  
Keyword(s):  
Dna Fingerprinting ◽  
Genetic Purity ◽  
Rice Varieties ◽  
Purity Assessment

scholarly journals Genetic purity certificate in seeds of hybrid maize using molecular markers

2006 ◽  
Vol 28 (1) ◽  
pp. 169-175 ◽  
Author(s):  
Kalinka Carla Padovani de Carvalho Salgado ◽  
das Graças Guimarães Carvalho Vieira ◽  
Édila Vilela de Resende Von Pinho ◽  
Cláudia Teixeira Guimarães ◽  
Renzo Garcia Von Pinho ◽  
...  
Keyword(s):  
Isocitrate Dehydrogenase ◽  
Female Parent ◽  
Parental Line ◽  
Maize Breeding ◽  
Genetic Purity ◽  
Mendelian Segregation ◽  
Purity Analysis ◽  
Young Leaves ◽  
Parental Lines ◽  
Hybrid Maize

One of the main features that confer high quality to the seed is its genetic purity, in which one of the major causes of contamination is the self-pollination of the female parent. Up to date, there is no accurate and fast methods for detecting such contamination. Thus, this work was carried out to certify the genetic purity in seeds of hybrid maize using different biochemical and DNA-based markers. Two single-cross hybrids and their parental lines derived from the maize breeding program at UFLA were evaluated by isoenzymatic pattern of alcohol dehydrogenase (ADH), esterase (EST), acid phosphatase (ACP), glutamate-oxaloacetate transaminase (GOT), malate dehydrogenase (MDH), isocitrate dehydrogenase (IDH), phosphoglucomutase (PGM), 6-phosphoglucomate dehydrogenase (PGDH), catalase (CAT) and ß-glucosidade (ßGLU) and by microsatellites markers. The enzymatic systems that were able to distinguish the hybrids from their parental line were the catalase, the isocitrate dehydrogenase and the esterase. The esterase showed a Mendelian segregation pattern for UFLA 8/3 hybrid, that enables a safer genetic purity certificate. Microsatellites were able to differentiate the hybrid lines and the respective parental lines. Moreover, this technique was fast, precise and without environment effects. For microsatellites, the amplification pattern was identical when young leaves or seeds were used as DNA source. The possibility of using seeds as DNA source would accelerate and facilitate the role process of the genetic purity analysis.

Assessment of genetic purity of tomato (Lycopersicon esculentum L.) hybrid using molecular markers

2007 ◽  
Vol 115 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Li-Wang Liu ◽  
Yan Wang ◽  
Yi-Qin Gong ◽  
Tong-Min Zhao ◽  
Guang Liu ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Lycopersicon Esculentum ◽  
Genetic Purity

scholarly journals Genetic Diversity Analysis of Hybrid Rice Parental Lines and Genetic Purity Assessment of Hybrid Seeds of China

2020 ◽  
Vol 12 (5) ◽  
pp. 37
Author(s):  
Haiya Cai ◽  
Yuxia Lu ◽  
Gang Liu ◽  
Shuo Zhang ◽  
Haitao Jia ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Hybrid Rice ◽  
Southern China ◽  
Polymorphic Information Content ◽  
Diversity Analysis ◽  
Japonica Rice ◽  
Similarity Coefficients ◽  
Genetic Purity ◽  
Genetic Diversity Analysis ◽  
Parental Lines

Thirty-five pairs of SSR primers were used for genetic diversity analysis and DNA fingerprinting of 31 hybrid rice core parental lines developed in central- and southern-China using one japonica rice line and three inbred rice lines as the check varieties. The average number of alleles (Na) per SSR locus was 4.02, with a range of two to eight, the effective number of alleles (Ne) was 83.16 with a mean of 2.38, ranging from 1.19 to 4.66. The polymorphic information content (PIC) ranged from 0.16 to 0.79, with an average number of 0.52. The results of the cluster analysis indicated that the check varieties viz., one japonica rice and three inbred rice, were clustered into two groups with similarity coefficients of 0.62 and 0.71 respectively indicating their relatedness. Thirty-one hybrid rice parental lines were clustered into 6 groups according to their different types, pedigrees and regions of development with similarity coefficients of approximately 0.76. The highest genetic similarity coefficient (0.94) was observed between Y58S and C815S, and the lowest (0.63) was observed between Quan9311A and Peiai64S. The purity of one hybrid rice cultivar was tested using characteristic marker and the field test, and it was demonstrated that the purities obtained using the two methods were similar. This research will be helpful for rice breeding, new cultivar registration and seed production.

Sign in / Sign up

Export Citation Format

Share Document