scholarly journals A Supplement Containing Trans-10, Cis-12 Conjugated Linoleic Acid Reduces Milk Fat Yield but Does Not Alter Organ Weight or Body Fat Deposition in Lactating Ewes

2010 ◽  
Vol 140 (11) ◽  
pp. 1949-1955 ◽  
Author(s):  
Liam A. Sinclair ◽  
Weerasinghe M. P. B. Weerasinghe ◽  
Robert G. Wilkinson ◽  
Michael J. de Veth ◽  
Dale E. Bauman
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Body Fat ◽  
Milk Fat ◽  
Fat Deposition ◽  
Organ Weight ◽  
Fat Yield

The effect of trans- 10, cis- 12 conjugated linoleic acid on milk fat synthesis in lactating sheep

2005 ◽  
Vol 2005 ◽  
pp. 92-92 ◽  
Author(s):  
L. A. Sinclair ◽  
A. L. Lock ◽  
J. W. Perfield ◽  
B. M. Teles ◽  
D. E. Bauman
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Milk Fat ◽  
Milk Protein ◽  
Potent Inhibitor ◽  
Curvilinear Relationship ◽  
Fat Synthesis ◽  
Fat Yield ◽  
Protein Output ◽  
Milk Fat Synthesis

Trans- 10, cis- 12 conjugated linoleic acid (CLA), a biohydrogenation intermediate produced in the rumen, is a potent inhibitor of milk fat synthesis. Data from a number of studies where various doses of trans -10, cis -12 CLA have been abomasally infused demonstrate a curvilinear relationship between the percent reduction in milk fat yield and both the dose of trans- 10, cis- 12 CLA infused and the milk fat content of trans- 10, cis- 12 CLA. In addition to a reduction in milk fat output, under some circumstances an increase in milk yield and milk protein output are observed. To date, there has been no examination of the effects of trans- 10, cis- 12 CLA on milk fat synthesis in lactating sheep. The current study was therefore designed to determine if trans- 10, cis- 12 CLA would inhibit milk fat synthesis in lactating sheep. In order to test the effectiveness of trans- 10, cis- 12 CLA in inhibiting milk fat synthesis we used a lipid-encapsulated trans- 10, cis- 12 CLA supplement (LE-CLA) as a means to provide the trans- 10, cis- 12 CLA isomer post-ruminally.

scholarly journals Trans-10, Trans-12 Conjugated Linoleic Acid Does Not Affect Milk Fat Yield but Reduces Δ9-Desaturase Index in Dairy Cows

2006 ◽  
Vol 89 (7) ◽  
pp. 2559-2566 ◽  
Author(s):  
J.W. Perfield ◽  
P. Delmonte ◽  
A.L. Lock ◽  
M.P. Yurawecz ◽  
D.E. Bauman
Keyword(s):  
Linoleic Acid ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Milk Fat ◽  
Δ9 Desaturase ◽  
Fat Yield

scholarly journals Combined effects of trans-10,cis-12 conjugated linoleic acid, propionate, and acetate on milk fat yield and composition in dairy cows

2011 ◽  
Vol 94 (4) ◽  
pp. 2051-2059 ◽  
Author(s):  
G. Maxin ◽  
F. Glasser ◽  
C. Hurtaud ◽  
J.L. Peyraud ◽  
H. Rulquin
Keyword(s):  
Linoleic Acid ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Milk Fat ◽  
Combined Effects ◽  
Fat Yield

Isomerisation of trans-10 cis-12 conjugated linoleic acid during acidic methylation

2009 ◽  
Vol 2009 ◽  
pp. 146-146
Author(s):  
M R F Lee ◽  
J K S Tweed
Keyword(s):  
Immune Response ◽  
Linoleic Acid ◽  
Animal Models ◽  
Conjugated Linoleic Acid ◽  
Milk Fat ◽  
Health Benefits ◽  
Fat Deposition

Conjugated linoleic acid (CLA) has been associated with numerous health benefits in animal models including modulation of immune response and fat deposition (Lock & Bauman, 2004). The most predominant CLA in ruminant products (meat and milk) is cis-9 trans-11 with traces of other isomers such as trans-10 cis-12 which can be increased according to dietary regimes such as increasing the proportion of concentrate in the diet (Sackmann et al. 2003). More recent findings into the health benefits of CLA in humans have found the trans-10 cis-12 isomer to be more potent than cis-9 trans-11 (Tholstrup et al. 2008). Whilst it has been reported that acidic methylation during analysis of milk fat results in isomerisation of cis-9 trans-11 (Lee & Tweed, 2008) little information is available on the effect of different methylation regimes on the increasingly important trans-10 cis-12 isomer. This study investigated the isomerisation of esterified and un-esterified trans-10 cis-12 CLA during three methylation regimes.

Milk production responses of primiparous and multiparous dairy cows to dose of conjugated linoleic acid consumed in rumen inert form

2009 ◽  
Vol 89 (3) ◽  
pp. 393-399 ◽  
Author(s):  
M A Sippel ◽  
R S Spratt ◽  
J P Cant
Keyword(s):  
Linoleic Acid ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Milk Production ◽  
Milk Fat ◽  
Milk Composition ◽  
Latin Squares ◽  
Net Energy ◽  
Calcium Salts ◽  
Fat Yield

Conjugated linoleic acid (CLA) has the potential to be used as a dietary means of manipulating milk fat production of dairy cows to meet specified short-term targets, as in a supply-managed industry. An experiment was conducted to evaluate the dose-response to calcium salts of CLA fed as a top-dress on a corn- and alfalfa-based TMR. In two 5 × 5 Latin squares of 28-d periods, five primiparous and five multiparous cows were fed 0, 22, 45, 67 or 178 g d-1 CLA (29% trans-10, cis-12) as a calcium salt. Results from one multiparous cow on the 178 g d-1 treatment were removed from statistical analysis because milk production fell to 1.2 kg d-1 by the end of the period. On average, milk, lactose and protein yields increased at the lower doses of CLA and decreased to control levels at the highest dose. Milk fat yield declined progressively from 876 to 770, 689, 676 and 543 g d-1 on the five doses. Net energy balance of cows increased linearly from -1.5 Mcal d-1 on the control to 2.0 Mcal d-1 at 178 g d-1 CLA. The percentage depression in milk fat yield at different doses of trans-10, cis-12 CLA was described by the equation y = 54/(1 + 22.8/dose). Comparison with published literature suggested that feeding calcium salts of CLA was 30% as effective in depressing milk fat production as an abomasal infusion, due to rumen biohydrogenation and intestinal indigestibility.Key words: Milk composition, cattle, diet

scholarly journals Influence of Dietary Fish Oil on Conjugated Linoleic Acid and Other Fatty Acids in Milk Fat from Lactating Dairy Cows

2000 ◽  
Vol 83 (11) ◽  
pp. 2620-2628 ◽  
Author(s):  
D.C. Donovan ◽  
D.J. Schingoethe ◽  
R.J. Baer ◽  
J. Ryali ◽  
A.R. Hippen ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Fish Oil ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Milk Fat ◽  
Lactating Dairy Cows ◽  
Dietary Fish

scholarly journals Isomerisation of cis-9 trans-11 conjugated linoleic acid (CLA) to trans-9 trans-11 CLA during acidic methylation can be avoided by a rapid base catalysed methylation of milk fat

2008 ◽  
Vol 75 (3) ◽  
pp. 354-356 ◽  
Author(s):  
Michael R F Lee ◽  
John K S Tweed
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Milk Fat ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
The Other ◽  
Gas Liquid Chromatography ◽  
Freeze Dried ◽  
Acidic Conditions

This study investigated the evolution of trans-9 trans-11 conjugated linoleic acid (CLA) from cis-9 trans-11 CLA during methylation and its avoidance through a rapid base methylation of milk fat. The study examined three conditions shown to result in loss of cis-9 trans-11 CLA during methylation namely: temperature, methylation time, water contamination in old reagents and acidic conditions. Three techniques currently used for the conversion of milk fat into fatty acid methyl esters for analysis of CLA content by gas liquid chromatography and a fourth procedure designed to eliminate acidic conditions and to limit methylation temperature and time were used. The four methods were: (i) acidic methylation (AM); (ii) acidic and basic bimethylation with fresh reagents (FBM); (iii) acidic and basic bimethylation with pre-prepared reagents (PBM) and (iv) basic methylation (BM). Each regime was carried out on six milk samples over two periods and methylated 1 ml freeze-dried milk (n=12 per regime). Total CLA was not different across methylation regimes (0·30 mg/ml). Isomer cis-9 trans-11 was higher (P<0·01) with BM than the other regimes and lowest with AM: 21·2, 17·8, 18·8 and 14·7 mg/100 ml for BM, FBM, PBM and AM, respectively. The inverse relationship was shown for trans-9 trans-11 with higher (P<0·001) amounts with AM than the other regimes and lowest with BM: 0·57, 2·55, 2·36 and 3·69 mg/100 ml for BM, FBM, PBM and AM, respectively. The trans-10 cis-12 isomer was also shown to alter with methylation procedure being higher (P<0·001) with AM than the other regimes: 0·43, 0·47, 0·29 and 1·20 mg/100 ml for BM, FBM, PBM and AM, respectively. Validation with known CLA free fatty acid and triacylglycerol standards confirmed that AM resulted in conversion of cis-9 trans-11 to trans-9 trans-11, and also elevated trans-10 cis-12 whilst BM of triacylglycerol CLA did not isomerise cis-9 trans-11 and was comparable to FBM.

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