scholarly journals Distribution and changes in the sbGnRH system in Rastrelliger brachysoma males during the breeding season

2021 ◽  
Vol 85 (3) ◽  
pp. 187-195
Author(s):  
Sinlapachai Senarat ◽  
Jes Kettratad ◽  
Wannee Jiraungkoorskul ◽  
Niwat Kangwanrangsan ◽  
Masafumi Amano ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Gonadosomatic Index ◽  
Follicle Stimulating Hormone ◽  
Testicular Development ◽  
Mrna Levels ◽  
Candidate Species ◽  
In Captivity ◽  
The Difference ◽  
Breeding Seasons ◽  
Stimulating Hormone

Rastrelliger brachysoma is a mariculture candidate species, but reproduction in captive fish has been problematic. This report examines the difference in the HPG axis, the neuroendocrine system and the development of reproductive tissues between captive vs. wild male R. brachysoma. The gonadosomatic index (GSI) of sexually mature male wild R. brachysoma was 1.12±0.34 and 1.94±0.26 during the non-breeding and breeding seasons, respectively. Captive R. brachysoma had a GSI of 1.88±0.17. All wild R. brachysoma were in the late spermatogenic stage irrespective of seasons. Immunostaining results showed that sbGnRH-immunoreactive neurons were distributed in three areas of the brain, namely the nucleus periventricularis, nucleus preopticus and nucleus lateralis tuberis. Follicle stimulating hormone and luteinizing hormone immunoreactivities were also observed in the pituitary gland. The levels of brain sbGnRH and GtH mRNA were not significantly different between the non-breeding and breeding seasons, but captive fish displayed (times or percent difference) lower mRNA levels than wild fish. These results suggest that these hormones control the testicular development in R. brachysoma and that the impaired reproduction in captivity may be due to their relative lower expression levels of follicle stimulating hormone and luteinizing hormone genes.

DEXTRAN-COATED CHARCOAL USED IN THE RADIOIMMUNOASSAY OF HUMAN PITUITARY LUTEINIZING HORMONE

1973 ◽  
Vol 73 (3) ◽  
pp. 444-454 ◽  
Author(s):  
T. Sand ◽  
P. A. Torjesen
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Protein Composition ◽  
Thyroid Stimulating Hormone ◽  
Human Chorionic Gonadotrophin ◽  
Human Thyroid ◽  
Serum Samples ◽  
Human Pituitary ◽  
The Difference ◽  
Stimulating Hormone

ABSTRACT A radioimmunoassay for human pituitary luteinizing hormone (LH) using charcoal for the separation of free from antibody-bound hormone is described. The ability of the various types of charcoal preparations tested to separate free from antibody-bound hormone differed greatly as did the amount required to give maximum adsorption of free hormone. It was also found that the adsorption of free and antibody-bound hormone was greatly influenced by the presence of other proteins. Hence it was necessary to add human serum to the standard tubes before the addition of the charcoal-dextran suspension, in order to compensate for the difference in protein composition between the standards and the serum samples. Two antisera obtained from rabbits immunized with human chorionic gonadotrophin (HCG) were used. One of the antisera had an affinity to human thyroid-stimulating hormone (TSH) and human follicle-stimulating hormone (FSH) of less than 10% as compared to that of LH, while the other had an affinity of about 30 % as compared to that of LH.

Effects of body condition and environmental stress on ovulation rate, embryo survival, and associated plasma follicle stimulating hormone, luteinizing hormone, prolactin and progesterone profiles in Scottish Blackface ewes

1984 ◽  
Vol 38 (2) ◽  
pp. 201-209 ◽  
Author(s):  
S. M. Rhind ◽  
J. M. Doney ◽  
R. G. Gunn ◽  
I. D. Leslie
Keyword(s):  
Luteinizing Hormone ◽  
Body Condition ◽  
Follicle Stimulating Hormone ◽  
Survival Rates ◽  
Ovulation Rate ◽  
Embryo Survival ◽  
Luteal Function ◽  
Endocrine Status ◽  
The Difference ◽  
Stimulating Hormone

ABSTRACTIn a 2 × 2 factorial experiment, half of each of two groups of ewes in high (20 ewes) or low body condition (20 ewes) were subjected to procedures designed to simulate normal management and climatic stresses, and the effects of these treatments on ovulation rate, embryo survival and endocrine status were investigated.The mean ovulation rate of ewes in the high condition group was significantly higher than that of ewes in the low condition group (1·8 v. 11) (P < 0·001). Embryo survival rates were unaffected. Neither ovulation rate nor embryo survival were affected by stressful treatments.Circulating follicle stimulating hormone, luteinizing hormone and prolactin levels were recorded in the peri-ovulatory period. Mean circulating follicle stimulating hormone levels were similar in three of the treatment groups but were generally lower in ewes in the low condition/stressed group. This difference was significant in some of the sampling periods. Neither basal levels of luteinizing hormone nor the size of the pre-ovulatory luteinizing hormone surge were significantly affected by level of body condition or stress but the surge began earlier in ewes in the low condition groups. The difference in timing was not, however, related to ovulation rate. Circulating prolactin levels were consistently and often significantly lower in ewes in poor condition (P < 0·05). Levels were not significantly affected by stress.While ovulation rate was affected by body condition, the recorded progesterone profiles during the first 2 weeks after mating suggest that luteal function was not affected by any of the treatments applied.

scholarly journals The IGF1 Receptor Is Involved in Follicle-Stimulating Hormone Signaling in Porcine Neonatal Sertoli Cells

2019 ◽  
Vol 8 (5) ◽  
pp. 577 ◽  
Author(s):  
Rossella Cannarella ◽  
Iva Arato ◽  
Rosita A. Condorelli ◽  
Giovanni Luca ◽  
Federica Barbagallo ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Follicle Stimulating Hormone ◽  
Inhibin B ◽  
Testicular Development ◽  
Mrna Levels ◽  
Mediated Effects ◽  
Igf1r Inhibitor ◽  
Pre Treatment ◽  
Igf1 Receptor ◽  
Stimulating Hormone

Experimental evidence has shown that the IGF1 receptor (IGF1R) is involved in testicular development during embryogenesis. More recently, data gathered from mice granulosa cells and zebrafish spermatogonia suggest that IGF1R has a role in Follicle-stimulating hormone (FSH) signaling. No evidence has been reported on this matter in Sertoli cells (SCs) so far. The aim of the study was to evaluate the role, if any, of the IGF1R in FSH signaling in SCs. The effects of FSH exposure on myosin-phosphatase 1 (MYPT1), ERK 1/2, AKT308, AKT473, c-Jun N-terminal kinase (JNK) phosphorylation and on anti-Müllerian hormone (AMH), inhibin B and FSH receptor (FSHR) mRNA levels were assessed with and without the IGF1R inhibitor NVP-AEW541 in purified and functional porcine neonatal SCs. Pre-treatment with NVP-AEW541 inhibited the FSH-induced MYPT1 and ERK 1/2 phosphorylation, decreased the FSH-dependent Protein kinase B (AKT)308 phosphorylation, but did not affect the FSH-induced AKT473 and JNK phosphorylation rate. It also interfered with the FSH-induced AMH and FSHR down-regulation. No influence was observed on the FSH-stimulated Inhibin B gene expression. Conclusion. These findings support the role of theIGF1R in FSH signaling in porcine SCs. The possible influence of IGF1 stimulation on the FSH-mediated effects on SCs should be further explored.

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