ANALYSIS OF SULFACHLOROPYRIDAZINE AND SULFAPYRIDINE BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH MOLECULARLY IMPRINTED POLYMER AS THE STATIONARY PHASE

2009 ◽  
Vol 21 (06) ◽  
pp. 457-460 ◽  
Author(s):  
Tzu-Tai Lee ◽  
Ing-Bang Huang ◽  
Ching-Chiang Hwang
Keyword(s):  
Stationary Phase ◽  
Molecularly Imprinted Polymers ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Liquid Chromatographic Method ◽  
Separation Factors ◽  
Liquid Chromatographic

A simple and sensitive high performance liquid chromatographic method for simultaneous determination of sulfachloropyridazine and sulfapyridine has been developed. This method was developed by using self-prepared molecularly imprinted polymers (MIPs) as the stationary phase. The polymers were prepared by a noncovalent method with sulfachloropyridazine as the template, methacrylic acid as the functional monomer and ethylene glycoldimethacrylate as the cross-linker in the presence of chloroform as the solvent. In order to compare the chromatographic data from the stationary phase, the retention time of sulfachloropyridazine and sulfapyridine was given. The separation factors (α) were 1.79–1.89 that showed that the MIPs were able to recognize sulfachloropyridazine and sulfapyridine.

HPLC separation of ibuprofen and mefenamic acid using molecularly imprinted polymer as stationary phase

e-Polymers ◽  
2006 ◽  
Vol 6 (1) ◽  
Author(s):  
Chin-Yin Hung ◽  
Yun-Tzu Huang ◽  
Han-Hung Huang ◽  
Ching-Chiang Hwang
Keyword(s):  
Stationary Phase ◽  
Molecularly Imprinted Polymers ◽  
Mefenamic Acid ◽  
High Performance ◽  
Bulk Material ◽  
High Performance Liquid Chromatographic ◽  
Molecularly Imprinted ◽  
Functional Monomers ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

AbstractA simple high-performance liquid chromatographic method for the assay of ibuprofen and mefenamic acid in tablets is described. This method was developed by using self-prepared molecularly imprinted polymers (MIPs) as the stationary phase. MIPs are synthesized using non-covalent method by the free radical polymerization of template, functional monomer and an excess of crosslink monomers resulting in organic materials. The resulting bulk material was then ground into 25~44μm particles and packed into analytical columns. The effect of functional monomers/crosslinking ratio and the concentration of acetonitrile in the mobile phase were studied. Ibuprofen and mefenamic acid were efficiently resolved on the polymer. The retention time of ibuprofen and mefenamic acid were approximately 19.69±0.36min and 46.27±1.23min, respectively. Linearity was observed from 3.13-8.33g/l for ibuprofen and 0.3-0.9g/l for mefenamic acid, with the coefficients of determination (r2) of 0.9977 and 0.9958. The limits of detection (LOD) of ibuprofen and mefenamic acid were found to be respectively 0.42g/l and 0.06g/l, while the limits of quantitation (LOQ) were 1.41g/l and 0.20g/l, respectively. The self-prepared MIP was successfully applied to the commercial tablet analysis and the result showed a good accuracy with -1.33 ~ -1.8% and -0.8 ~ +1.33% for ibuprofen and mefenamic acid.

Quantitative Analysis of 5-Hydroxymethylfurfural in Linezolid Injection by High Performance Liquid Chromatography

2020 ◽  
Vol 16 (8) ◽  
pp. 1059-1067
Author(s):  
Jéssica Maurício Batista ◽  
Christian Fernandes
Keyword(s):  
High Performance ◽  
Potassium Phosphate ◽  
Gradient Elution ◽  
High Performance Liquid Chromatographic ◽  
Official Method ◽  
Ph Variation ◽  
Drug Products ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Background: Linezolid is a synthetic broad-spectrum antibacterial belonging to the class of oxazolidinones. Linezolid for intravenous infusion is isotonized with dextrose. In acidic environment, the dehydration of dextrose produces furan derivatives, 5-hydroxymethylfurfural (5-HMF) being the main one. The determination of this degradation product is of fundamental importance, since there is evidence it is cytotoxic, genotoxic, mutagenic and carcinogenic. However, there is no official method for the determination of 5-HMF in drug products. Objective: The aim of this study was to develop and validate a high performance liquid chromatographic method to quantify 5-HMF in injection of linezolid. Methods: The chromatographic separation, after optimization, was performed on C18 (150 x 4.6 mm, 5 μm) column. Mobile phase was composed of 14 mM potassium phosphate buffer pH 3.0 ([H+] = 1.0 x 10-3) and methanol in gradient elution at 1.0 mL min-1. The injection volume was 10 μL and detection was performed at 285 nm. Results: The method was optimized and validated, showing selectivity, linearity in the range from 0.075 to 9.0 μg mL-1, precision (RSD ≤ 2.0%), accuracy (mean recovery of 100.07%) and robustness for temperature and pH variation. Conclusion: The method was shown to be adequate to determine 5-HMF in injection containing linezolid in routine analysis.

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