scholarly journals Strategies for vegetative propagation and viral cleaning of a miniature ornamental pineapple hybrid

2021 ◽  
Vol 43 ◽  
pp. e53097
Author(s):  
Bruna de Fátima Batista da Silva ◽  
Everton Hilo de Souza ◽  
Rafaelle Souza de Oliveira ◽  
Carlos Alberto da Silva Ledo ◽  
Fernanda Vidigal Duarte Souza
Keyword(s):  
Vegetative Propagation ◽  
Nodal Segments ◽  
The Third ◽  
Healthy Mother ◽  
Mother Plants ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Propagation Methods ◽  
Pineapple Mealybug

This study assessed and compared different methods for vegetative propagation of a miniature ornamental pineapple hybrid (ORN-MUT), seeking to determine the best method for production of plantlets, as well as for removal of the PMWaV viral complex from plants cultured in vitro, for production of healthy parent plants. Pineapple wilt is a disease that can cause large economic and is caused by a viral complex called Pineapple mealybug wilt-associated virus (PMWaV). For this, four propagation methods were evaluated (conventional, stem sectioning, micropropagation and etiolation of nodal segments). The time necessary for each method and the number of plants formed were assessed. Stem tips (0.5 mm) were cultured and indexed for three PMWaV types. Conventional propagation produced 17 plantlets per plant in 566 days, stem sectioning produced 2.3 plantlets per stem in 591 days, while the conventional micropropagation technique produced 1,284 plants after four subcultures in 778 days. Stems etiolated for 60 days showed peak production in the second subculture, with 1,224 plants. This method required 883 days to obtain plants with ideal size for transplantation to the field. In turn, stems etiolated for 120 days produced 935 plants at the end of four subcultures, with peak output in the third subculture, in which the plants could be cultivated in the field after 943 days. Conventional micropropagation and etiolation for 60 days were the best methods for production of plantlets of the ORN-MUT hybrid. The results of this work showed that the cultivation of shoot tips is an efficient strategy to remove the PMWaV complex and obtain healthy mother plants and can be a useful tool for other varieties of pineapple.

scholarly journals Spatial and Temporal Incidences of Pineapple mealybug wilt-associated viruses in Pineapple Planting Blocks

Plant Disease ◽  
2010 ◽  
Vol 94 (2) ◽  
pp. 196-200 ◽  
Author(s):  
D. M. Sether ◽  
W. B. Borth ◽  
M. J. Melzer ◽  
J. Hu
Keyword(s):  
Ananas Comosus ◽  
Ratoon Crop ◽  
Virus Incidence ◽  
Central Regions ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug ◽  
Fruit Harvest ◽  
Propagation Material

A complex of Pineapple mealybug wilt-associated viruses (PMWaVs) that can infect pineapple (Ananas comosus) is correlated with reduced yields and mealybug wilt of pineapple. The incidences of PMWaV-1 and PMWaV-2 at planting, fruit harvest, the beginning of the ratoon crop, and ratoon fruit harvest were determined for end, side, and central regions of planting blocks in eight commercial fields. Differences in virus incidence for the three regions at ratoon harvest were highly significant (P = 0.0018). Central regions of planting blocks had lower virus incidences at the time of ratoon fruit harvest. Collection of propagation material from the central regions of planting blocks will help to minimize PMWaV incidence in fields planted with this material.

scholarly journals Probing behavior of Dysmicoccus brevipes mealybug in pineapple plants1

2016 ◽  
Vol 46 (4) ◽  
pp. 458-463
Author(s):  
Lenira Viana Costa Santa-Cecília ◽  
◽  
Ernesto Prado ◽  
Brígida Souza ◽  
Keyword(s):  
Insect Vector ◽  
Recording Time ◽  
Defense Strategies ◽  
Phenolic Contents ◽  
Probing Behavior ◽  
Epg Technique ◽  
Dysmicoccus Brevipes ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug

ABSTRACT Differences in susceptibility to viruses in plants may be partially explained by the insect vector probing behavior and by the presence of phenolic compounds, which are often associated with defense strategies. This study aimed at detecting barriers that may difficult the probing activity of the Dysmicoccus brevipes (Pseudococcidae) pineapple mealybug, a vector of the pineapple mealybug wilt-associated virus, as well as evaluating the phenolic content of plants, in order to verify any possible relationship with the probing behavior, by using the electrical penetration graphs (EPG) technique. Seedlings of 'Smooth Cayenne' and 'Pérola' pineapple cultivars were used in the experiments. Only 28 % and 21 % of the mealybugs reached the phloem of the 'Smooth Cayenne' and 'Pérola' cultivars, respectively, over 16 h of recording, with an average of 9 h to reach the phloem vessels. The xylem phase was extended in both cultivars and represented approximately 31 % ('Smooth Cayenne') and 44 % ('Pérola') of the recording time. The phenolic contents of both cultivars were similar.

PINEAPPLE MEALYBUG WILT ASSOCIATED VIRUS AND MEALYBUG WILT OF PINEAPPLE

2005 ◽  
pp. 209-212 ◽  
Author(s):  
J.S. Hu ◽  
D.M. Sether ◽  
M.J. Metzer ◽  
E. Pérez ◽  
A. Gonsalves ◽  
...  
Keyword(s):  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug

Further genomic characterization of pineapple mealybug wilt-associated viruses using high-throughput sequencing

2020 ◽  
Vol 45 (1) ◽  
pp. 64-72 ◽  
Author(s):  
James C. Green ◽  
Maher A. Rwahnih ◽  
Alejandro Olmedo-Velarde ◽  
Michael J. Melzer ◽  
Islam Hamim ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Genomic Characterization ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug

scholarly journals Molecular diagnosis of Fusarium guttiforme and Pineapple mealybug wilt-associated virus

2014 ◽  
Vol 8 (S4) ◽  
Author(s):  
Lorena Carnielli ◽  
Walkíria Andrade Amorim ◽  
Aline Vaz ◽  
Patricia Machado Bueno Fernandes ◽  
José Aires Ventura
Keyword(s):  
Molecular Diagnosis ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug

CHARACTERIZATION AND CONTROL OF PINEAPPLE MEALYBUG WILT ASSOCIATED AMPELOVIRUSES

2006 ◽  
pp. 23-27 ◽  
Author(s):  
E.P. Perez ◽  
D.M. Sether ◽  
M.J. Melzer ◽  
J.L. Busto ◽  
C. Nagai ◽  
...  
Keyword(s):  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug ◽  
And Control

scholarly journals Differentiation, Distribution, and Elimination of Two Different Pineapple mealybug wilt-associated viruses Found in Pineapple

Plant Disease ◽  
2001 ◽  
Vol 85 (8) ◽  
pp. 856-864 ◽  
Author(s):  
D. M. Sether ◽  
A. V. Karasev ◽  
C. Okumura ◽  
C. Arakawa ◽  
F. Zee ◽  
...  
Keyword(s):  
Agricultural Research ◽  
The United States ◽  
United States Department ◽  
Rt Pcr ◽  
Apical Bud ◽  
The World ◽  
Pcr Assays ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug

Surveys for Pineapple mealybug wilt-associated virus-1 (PMWaV-1) and PMWaV-2 were conducted on pineapple samples from Hawaii and around the world. Tissue blot immunoassays (TBIAs) with two different monoclonal antibodies (MAb) specific to either PMWaV-1 or PMWaV-2 indicated that both closteroviruses are widely distributed throughout the pineapple-growing areas of the world. In the worldwide survey, PMWaV-1 was found in 80% of the mea-lybug wilt of pineapple (MWP)-symptomatic and 78% of the asymptomatic pineapple plants tested. A subset of plants was tested for PMWaV-2; 100% of the symptomatic plants and 12% of the asymptomatic plants were positive for this virus. A reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to differentiate between PMWaV-1 and PMWaV-2. Oligonucleotide primers were designed using distinct regions of the HSP 70 homolog genes of the two viruses. PMWaV-specific RT-PCR assays and TBIAs were used to screen the pineapple accessions maintained at the United States Department of Agriculture-Agricultural Research Service National Clonal Germplasm Repository for PMWaV infection; 73% of the accessions were found infected with at least one PMWaV. Pineapple accessions found PMWaV-free were challenged with viruliferous mealybugs to test for immunity to PMWaV-1. No immune germ plasm was identified. Potential alternative virus hosts were screened for infection with virus-specific RT-PCR assays and TBIAs and were also challenged with viruliferous mealybugs. No alternate hosts of PMWaV-1 or PMWaV-2 were identified. PMWaV-1 infection was eliminated through axillary and apical bud propagation from infected crowns. Strategies to manage MWP are discussed.

scholarly journals In vitro germination and vegetative propagation through bud development of sacha inchi (Plukenetia volubilis L.)

2021 ◽  
Vol 27 (1) ◽  
Author(s):  
Ana María Henao Ramírez ◽  
Aura Inés Urrea Trujillo ◽  
Lucía Atehortúa Garcés
Keyword(s):  
Seed Germination ◽  
Seed Coat ◽  
Vegetative Propagation ◽  
Plant Production ◽  
Nodal Segments ◽  
Oilseed Crop ◽  
Bud Development ◽  
Plukenetia Volubilis ◽  
Sacha Inchi

This study describes the in vitro seed germination and micropropagation of Plukenetia volubilis (sacha inchi), an oilseed crop rich in omega-3 fatty acids, with health benefits and several industrial applications. Seed germination was evaluated in different culture media (MS and 1/2 MS), seed coat presence/absence and culture temperature (18 °C and 28 °C). Micropropagation was performed using axillary bud development (ABD) on nodal segments from in vitro seedlings. KIN, BAP and 2-ip were evaluated for ABD, and the effect of modified MS in 453 mg L-1 CaCl2 and 351.62 mg L-1 MgSO4 on ABD and shoot survival was assessed to improve the process. Finally, six treatments were evaluated to optimize ABD and shoot leaf formation. Seed germination of 91.6 % was achieved in MS at 28 °C when the seed coat was removed. ABD was obtained in 45 % and 40 % with 0.4 mg L-1 KIN and 0.6 mg L-1 2-ip, respectively, with the least CAL. The modification in 453 mg L-1 CaCl2 then allowed 76 % ABD and 82 % explant survival. ABD response was optimized to 95 % and 2.45 leaves with MS medium + CaCl2 modification + 10 % coconut water + 0.4 mg L-1 KIN. The same results were obtained by replacing the latter with 0.6 mg L-1 2-ip. Rooting was achieved in MS without PGR, and acclimatization was successful. The results indicate that plant production via germination and vegetative propagation is effective for commercial purposes.

Sign in / Sign up

Export Citation Format

Share Document