scholarly journals In vitro germination and vegetative propagation through bud development of sacha inchi (Plukenetia volubilis L.)

2021 ◽  
Vol 27 (1) ◽  
Author(s):  
Ana María Henao Ramírez ◽  
Aura Inés Urrea Trujillo ◽  
Lucía Atehortúa Garcés
Keyword(s):  
Seed Germination ◽  
Seed Coat ◽  
Vegetative Propagation ◽  
Plant Production ◽  
Nodal Segments ◽  
Oilseed Crop ◽  
Bud Development ◽  
Plukenetia Volubilis ◽  
Sacha Inchi

This study describes the in vitro seed germination and micropropagation of Plukenetia volubilis (sacha inchi), an oilseed crop rich in omega-3 fatty acids, with health benefits and several industrial applications. Seed germination was evaluated in different culture media (MS and 1/2 MS), seed coat presence/absence and culture temperature (18 °C and 28 °C). Micropropagation was performed using axillary bud development (ABD) on nodal segments from in vitro seedlings. KIN, BAP and 2-ip were evaluated for ABD, and the effect of modified MS in 453 mg L-1 CaCl2 and 351.62 mg L-1 MgSO4 on ABD and shoot survival was assessed to improve the process. Finally, six treatments were evaluated to optimize ABD and shoot leaf formation. Seed germination of 91.6 % was achieved in MS at 28 °C when the seed coat was removed. ABD was obtained in 45 % and 40 % with 0.4 mg L-1 KIN and 0.6 mg L-1 2-ip, respectively, with the least CAL. The modification in 453 mg L-1 CaCl2 then allowed 76 % ABD and 82 % explant survival. ABD response was optimized to 95 % and 2.45 leaves with MS medium + CaCl2 modification + 10 % coconut water + 0.4 mg L-1 KIN. The same results were obtained by replacing the latter with 0.6 mg L-1 2-ip. Rooting was achieved in MS without PGR, and acclimatization was successful. The results indicate that plant production via germination and vegetative propagation is effective for commercial purposes.

Vegetative propagation of the underutilized oilseed crop sacha inchi (Plukenetia volubilis L.)

2018 ◽  
Vol 65 (7) ◽  
pp. 2027-2036 ◽  
Author(s):  
Danter Huansi Cachique ◽  
Henry Ruiz Solsol ◽  
Marco Antonio García Sanchez ◽  
Luis Alberto Arévalo López ◽  
Nete Kodahl
Keyword(s):  
Vegetative Propagation ◽  
Oilseed Crop ◽  
Plukenetia Volubilis ◽  
Sacha Inchi

scholarly journals Desempenho horticultural de plantas propagadas in vitro de Sacha inchi

2014 ◽  
Vol 44 (6) ◽  
pp. 1050-1053 ◽  
Author(s):  
Paulo Hercílio Viegas Rodrigues ◽  
Stevan Ricardo Bordignon ◽  
Glaucia Maria Bovi Ambrosano
Keyword(s):  
Plukenetia Volubilis ◽  
Sacha Inchi

O objetivo do presente trabalho foi avaliar, em condições de campo, características horticulturais de mudas propagadas in vitro de Sacha inchi (Plukenetia volubilis L), quanto à época de florescimento e colheita, características morfológicas relacionadas à coloração e forma de folhas, caule e frutos, bem como a produtividade do material propagado in vitro. As mudas de cultura de tecidos foram propagadas por seis semanas em meio de cultivo com sais e vitaminas de MS, acrescido de 30g L-1 de sacarose e 1,0mg L-1 de BAP, a partir do ponteiro de plântula germinada in vitro. Foram selecionadas, ao acaso, quatro plantas propagadas in vitro, quatro plantas por via seminal e mais vinte e duas plantas, também por via seminal, compondo a bordadura, totalizando trinta plantas. As análises foram semanais até completar quatorze meses do plantio, que ocorreu antes da primeira poda. Os resultados obtidos indicam a não ocorrência de variabilidade genética nas plantas produzidas in vitro, bem como precocidade na produção e maior produtividade, quando comparado com o material convencional obtido via seminal.

scholarly journals Efeito inibitório de extrato alcoólico de amêndoa de Sacha-inchi em Rhizoctonia sp

2021 ◽  
Vol 14 (6) ◽  
pp. 27-30
Author(s):  
Renan Colavite Dos Santos ◽  
Ana Paula Rodrigues da Silva ◽  
Giseudo Aparecido Paiva ◽  
Ostenildo Ribeiro Campos ◽  
Adriana Matheus da Costa Figueiredo
Keyword(s):  
Plukenetia Volubilis ◽  
Sacha Inchi

A agricultura sustentável busca meios alternativos para controlar o ataque fúngico de acordo com as particularidades de cada fungo, com o intuito de reduzir o uso de agroquí­micos. Os extratos alcóolicos são uma alternativa, visto que são produtos naturais extraí­dos de plantas ou de partes da mesma que apresentam efeito fungicida. Desse modo o objetivo desse trabalho foi testar o controle in vitro do Rhizoctonia sp. utilizando diferentes concentrações de extrato alcoólicos de Sacha Inchi (Plukenetia volubilis). Para isso foi instalado um experimento em delineamento inteiramente ao acaso com 6 concentrações de extrato alcoólico de Sacha Inchi (0%, 2%, 4%, 6%, 8% e 10%) com 8 repetições. Foram mensurados o crescimento diário da colônia, o í­ndice de velocidade de crescimento micelial (IVCM) e a porcentagem de inibição de crescimento (PIC). Os dados obtidos foram analisados via analise de regressão polinomial utilizando o software R Studio. A partir da concentração de 6% o fungo apresenta pequeno desenvolvimento, e na dose máxima testada (10%) o crescimento do fungo foi praticamente nulo. O extrato alcoólico de Sacha Inchi foi eficiente a partir da concentração de 6%, o que o torna indicado para o controle do fungo Rhizoctonia sp

scholarly journals Caracterización químico nutricional de tres semillas de oleaginosas disponibles en la región Amazonas

2021 ◽  
Vol 2 (3) ◽  
pp. 24
Author(s):  
Katerin Goñas ◽  
Wilmer Bernal
Keyword(s):  
Ricinus Communis ◽  
Salvia Hispanica ◽  
Plukenetia Volubilis ◽  
Sacha Inchi

<p>El objetivo del presente trabajo fue la caracterización de tres semillas de oleaginosas de la región Amazonas. Los siguientes parámetros evaluados fueron: humedad (H), proteína cruda (nitrógeno total) (%PT), cenizas (CZA%), fibra cruda (FC), extracto etéreo (EE), extracto libre de nitrógeno (ELN). Además se determinó energía bruta (EB), fibra detergente acida (FDA), fibra detergente neutra (FDN%), porcentaje de calcio (Ca) y fosforo (P) y digestibilidad in vitro. Las semillas de oleaginosas fueron semilla de Sacha Inchi (Plukenetia volúbilis linneo ) semilla de Higuerilla (Ricinus communis) y semilla de Chía {salvia hispánica). La clasificación final de dichos insumos se realizó por la concentración de nutrientes en proteicos, energéticos y fibrosos. Concluyendo que en la región Amazonas las semillas de oleaginosas poseen un alto potencial nutricional para la alimentación animal. Sin embargo, es necesario la caracterización del perfil de aminoácidos y componentes anti nutricionales en los insumos identificados.</p>

scholarly journals In vitro propagation of sacha inchi through organogenesis

2018 ◽  
Vol 53 (11) ◽  
pp. 1285-1288 ◽  
Author(s):  
Reynaldo Solis ◽  
Danter Cachique ◽  
Juan Carlos Guerrero-Abad ◽  
María Emilia Ruiz Sánchez ◽  
Lourdes Tapia y Figueroa
Keyword(s):  
In Vitro Propagation ◽  
Naphthaleneacetic Acid ◽  
Indolebutyric Acid ◽  
In Vitro Organogenesis ◽  
Apical Meristems ◽  
Hormonal Balance ◽  
Plukenetia Volubilis ◽  
Sacha Inchi

Abstract: The objective of this work was to evaluate hormonal balance in the phases of the in vitro organogenesis of apical meristems of sacha inchi (Plukenetia volubilis). For the establishment and multiplication phases, Murashige & Skoog (MS) media, with different concentrations of benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), were used. For rooting, modified MS media was supplemented with different concentrations of NAA and indolebutyric acid. The differentiation of apical meristems was possible with 0.1 mg L-1 BAP and 0.05 mg L-1 NAA. The best rooting of seedlings is obtained with 0.5 mg L-1 naphthaleneacetic acid and 2.0 mg L-1 indolebutyric acid.

scholarly journals Environmental factors on seed germination, seedling survival and initial growth of sacha inchi (Plukenetia volubilis L.)

2015 ◽  
Vol 37 (2) ◽  
pp. 111-116 ◽  
Author(s):  
Amanda Ávila Cardoso ◽  
Amana de Magalhães Matos Obolari ◽  
Eduardo Euclydes de Lima e Borges ◽  
Cristiane Jovelina da Silva ◽  
Haroldo Silva Rodrigues
Keyword(s):  
Seed Germination ◽  
Seedling Survival ◽  
Continuous Light ◽  
Initial Growth ◽  
Omega 3 ◽  
Continuous Darkness ◽  
Omega 6 ◽  
Plukenetia Volubilis ◽  
Sacha Inchi ◽  
Continuous Temperature

Abstract: Sacha inchi (Plukenetia volubilis L.) is an Amazon species of elevated agro-industrial potential due the high content of omega-3 and omega-6 in its seeds. Despite of it, little information about its propagation by seeds is currently available. Thus, the aim of this study was to assess seed germination, seedling survival and growth of this species under different conditions of substrate (on paper, between papers and paper roll), light (continuous darkness, 12-h photoperiod and continuous light) and temperature (continuous temperature at 20, 25, 30, 35 and 40 °C). Germination is stimulated by substrates with increased surface contact with the seeds, presence of light and temperatures between 25 and 35 °C. Survival and initial growth of seedlings are favored by vermiculite, continuous light and 30 °C temperature. These conditions allow rapid and uniform germination of seeds and better establishment and development of seedlings. We encourage the propagation of sacha inchi by seeds, since we consider it a feasible technique.

scholarly journals Allelopathic effect of aqueous extracts from agro-industrial residues of pecan nut [Carya illinoinensis (Wangenh) C. Koch] and pinhão (Araucaria angustifolia)

2017 ◽  
Vol 3 (3) ◽  
pp. 495-507 ◽  
Author(s):  
Voltaire SANT'ANNA ◽  
Maico Ismael KLEIN ◽  
Elaine BIONDO ◽  
Eliane Maria KOLCHINSKI
Keyword(s):  
Seed Germination ◽  
Seed Coat ◽  
Plant Root ◽  
In Vitro Tests ◽  
Allelopathic Effect ◽  
Lettuce Seed ◽  
Aqueous Extracts ◽  
Industrial Residues ◽  
Pecan Nutshell

Pinhão seed coat and pecan nutshell are residues widely produced in households and industries, which present great potential to be used as source of natural preservatives in the food chain. The objective of the present study is to evaluate the effectiveness of pinhão seed coat and pecan nutshell aqueous extracts in inhibiting seed germination. Extracts were obtained in concentration of 10g/L, 5g/L and 2.5g/L and tested against lettuce seed germination in vitro tests. The results show that both aqueous extract presented the significant (p<0.05) ability to inhibit seeds germination as well as the germination seed index, in relation to control experiments, when used in concentrations of up to 5g/L. Extracts did not interfere significantly (p>0.05) the germinated plant root size. Thus, aqueous extracts of agro-industrial residues from pinhão seed coat and pecan nutshell presents alellopathic activity, showing up as interesting alternative to be used as natural herbicide, also representing a way of solid waste management by industries and small farmers.

scholarly journals Non-symbiotic Seed Germination and In vitro Plant Development of Pholidota articulata

2021 ◽  
Vol 15 ◽  
pp. 44-51
Author(s):  
R. Devendra Prasad ◽  
Shreeti Pradan ◽  
Mukti Ram Poudel ◽  
Bijaya Pant
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Plant Production ◽  
Sustainable Utilization ◽  
Ms Medium ◽  
Natural Habitats ◽  
Half Strength ◽  
Indole 3 Acetic Acid ◽  
Symbiotic Seed Germination

Pholidota articulata is an epiphytic orchid mostly used in ornamental cut/pot flower and in traditional medicine. As it has high ornamental and medicinal values, its population from natural habitats is decreasing, therefore, it is listed in the Appendix-II of Convention on International Trade in Endangered Species (CITES). The objective of the present study is to obtain the in vitro plants of P. articulata from seed culture to conserve its germplasm. The in vitro seed germination was carried out in different strengths of Murashige and Skoog (MS) and Knudson C (KnC) medium supplemented with various plant hormones. On the half-strength of MS medium, seeds were started to germinate after 4 weeks of primary culture and they were developed into protocorms with first leaf primordium earlier than on the other medium. Therefore, in vitro developed protocorms were sub-cultured on the half-strength of MS medium supplemented with different concentrations of 6-benzylaminopurine (BAP), gibberellic acid (GA3) and α-naphtalene acetic acid (NAA). They were successfully developed into shoots on the 1.5 mg/l BAP supplemented half-strength of MS medium. Later, they were inoculated on the half-strength of MS medium supplemented with different concentration of α-napthalene acetic acid (NAA), indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) for the root formation, where IBA supplemented medium was found effective for the development of roots. Thus, this study provides a reliable protocol for non-symbiotic seed germination and plant production, and reveals that seed-derived protocorms are good explants for the in vitro mass propagation for conservation and sustainable utilization in horticulture.

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