Phylogenetic characterization and quantification by Most Probable Number of the microbial communities of biomass from the Upflow Anaerobic Sludge Blanket Reactor under sulfidogenic conditions

The present work addresses the preparation of the effluent from a full-scale upflow anaerobic sludge blanket (UASB) reactor for drip irrigation of orange crops. The pilot plant included a lamella plate clarifier followed by a geo-textile blanket filter and a UV disinfection reactor. The clarifier operated with a surface load of 115 m3m−2d−1, whereas the filter operated with 10 m3m−2d−1. The UV reactor was an open-channel type and the effective dose was approximately 2.8 W h m−3. The effluent of the UASB reactor received 0.5 mg L−1 cationic polyelectrolyte before entering the high-rate clarifier. Suspended solids’ concentrations and Escherichia coli and helminth egg's densities were monitored throughout the treatment system for 12 months. Results showed that the total suspended solids concentration in the filter effluent was lower than 7 mg L−1 and helminth density was below 1.0 egg L−1. The UV disinfection demonstrated the ability to produce a final effluent with E. coli density lower than 103MPN/100 mL (MPN: most probable number) during the entire process. Thus, the World Health Organization standards for unrestricted crop use were met. Agronomic interest parameters were controlled and it was possible to identify the important contribution of treated sewage in terms of the main nutrients.

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Biomass granulation in an upflow anaerobic sludge blanket reactor treating 500 m3/day low-strength sewage and post treatment in high-rate algal pond

Water Science & Technology ◽

10.2166/wst.2017.269 ◽

2017 ◽

Vol 76 (5) ◽

pp. 1234-1242

Author(s):

Pritha Chatterjee ◽

M. M. Ghangrekar

Keyword(s):

Ammonia Removal ◽

Phosphate Removal ◽

High Rate ◽

Upflow Anaerobic Sludge Blanket ◽

Uasb Reactor ◽

Anaerobic Sludge ◽

Probable Number ◽

Moving Bed ◽

Anaerobic Sludge Blanket ◽

Final Effluent

A pilot-scale upflow anaerobic sludge blanket-moving bed biofilm (UASB-MBB) reactor followed by a high-rate algal pond (HRAP) was designed and operated to remove organic matter, nutrients and pathogens from sewage and to facilitate reuse. For an influent chemical oxygen demand (COD) concentration of 233 ± 20 mg/L, final effluent COD was 50 ± 6 mg/L. Successful biomass granulation was observed in the sludge bed of the upflow anaerobic sludge blanket (UASB) reactor after 5 months of operation. Ammonia removal in HRAP was 85.1 ± 2.4% with average influent and effluent ammonia nitrogen concentrations of 20 ± 3 mg/L and 3 ± 1 mg/L, respectively. Phosphate removal after treatment in the HRAP was 91 ± 1%. There was a 2–3 log scale pathogen removal after treatment in HRAP with most probable number (MPN) of the final effluent being 600–800 per 100 mL, which is within acceptable standards for surface irrigation. The blackwater after treatment in UASB-MBBR-HRAP is being reused for gardening and landscaping. This proper hydro-dynamically designed UASB reactor demonstrated successful granulation and moving bed media improved sludge retention in UASB reactor. This combination of UASB-MBB reactor followed by HRAP demonstrated successful sewage treatment for a year covering all seasons.

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Pemodelan Reaktor Hibrid Anaerob Pengolah Molasse Pada Pembebanan Organik Tinggi

10.31219/osf.io/q9dth ◽

2020 ◽

Author(s):

Gede H Cahyana

Keyword(s):

Fluidized Bed ◽

Fixed Bed ◽

High Rate ◽

Upflow Anaerobic Sludge Blanket ◽

Anaerobic Sludge ◽

Expanded Bed ◽

Anaerobic Sludge Blanket

Telah dikembangkan reaktor anaerob kecepatan tinggi (high rate) yang merupakan modifikasi reaktor konvensional. Di antaranya berupa (bio)reaktor pertumbuhan tersuspensi (contoh: UASB, Upflow Anaerobic Sludge Blanket) dan reaktor pertumbuhan lekat (Fixed Bed atau Biofilter, Fluidized Bed, Expanded Bed, Rotating Biodisc dan Baffled Reactor). Kedua tipe reaktor di atas memiliki sejumlah kelebihan dan kekurangan. Untuk mengoptimalkan nilai positifnya (terutama untuk keperluan desain) maka reaktor tersebut, pada penelitian ini, disusun menjadi satu urutan yang disebut Reaktor Hibrid Anaerob (Rehan) yakni UASB di bawah dan AF di atasnya. Lebih lanjut, penelitian ini diharapkan dapat memberikan informasi tentang kinerja Rehan dalam mengolah air limbah (substrat) yang konsentrasi zat organiknya (COD) sangat tinggi dan suatu model matematika yang dapat mewakili reaktor tersebut.

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The treatment of iron oxalate leach liquors in a UASB with sulfate reduction

Water Science & Technology ◽

10.2166/wst.1997.0614 ◽

1997 ◽

Vol 36 (6-7) ◽

pp. 383-390 ◽

Cited By ~ 1

Author(s):

J. E. Teer ◽

D. J. Leak ◽

A. W. L. Dudeney ◽

A. Narayanan ◽

D. C. Stuckey

Keyword(s):

Bacterial Species ◽

Upflow Anaerobic Sludge Blanket ◽

Anaerobic Sludge ◽

Desulfovibrio Vulgaris ◽

Hydrogenotrophic Methanogenesis ◽

Irreversible Effects ◽

High Concentrations ◽

A Minor ◽

Anaerobic Sludge Blanket ◽

Reducing Bacteria

The presence of small amounts of iron (>0.013% Fe) in sand creates problems in the manufacture of high quality glass. Removal by hot sulphuric acid is possible, but creates environmental problems, and is costly. Hence organic acids such as oxalic have been investigated since they are effective in removing iron, and can be degraded anaerobically. The aim of this work was to identify key intermediates in the anaerobic degradation of oxalate in an upflow anaerobic sludge blanket reactor (UASB) which was removing iron from solution in the sulphide form, and to determine the bacterial species involved. 2-bromoethanesulfonic acid (BES) and molybdenum were selected as suitable inhibitors for methanogenic and sulphate reducing bacteria (SRB) respectively. 40mM molybdenum was used to inhibit the SRB in a reactor with a 12hr HRT. Total SRB inhibition took place in 20 hrs, with a complete breakthrough of influent sulphate. The lack of an immediate oxalate breakthrough confirmed Desulfovibrio vulgaris subspecies oxamicus was not the predominant oxalate utilising species. Nevertheless, high concentrations of molybdenum were found to inhibit oxalate utilising bacteria in granular reactors but not in suspended population reactors; this observation was puzzling, and at present cannot be explained. Based on the intermediates identified, it was postulated that oxalate was degraded to formate by an oxalate utilising bacteria such as Oxalobacter formigenes, and the formate used by the SRBs to reduce sulphate. Acetate, as a minor intermediate, existed primarily as a source of cell carbon for oxalate utilising bacteria. Methanogenic inhibition identified that 62% of the CH4 in the reactor operated at 37°C originated from hydrogenotrophic methanogenesis, whilst this figure was 80% at 20°C. Possible irreversible effects were recorded with hydrogenotrophic methanogens.

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Biokinetics of an upflow anaerobic sludge blanket reactor treating whey permeate

Bioresource Technology ◽

10.1016/0960-8524(92)90006-j ◽

1992 ◽

Vol 41 (3) ◽

pp. 223-230 ◽

Cited By ~ 23

Author(s):

S.H. Hwang ◽

C.L. Hansen ◽

D.K. Stevens

Keyword(s):

Upflow Anaerobic Sludge Blanket ◽

Anaerobic Sludge ◽

Whey Permeate ◽

Anaerobic Sludge Blanket

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High process activity of a two-phase UASB (upflow anaerobic sludge blanket) receiving ethanol wastewater: Operational conditions in relation to granulation development

Biomass and Bioenergy ◽

10.1016/j.biombioe.2021.106012 ◽

2021 ◽

Vol 148 ◽

pp. 106012

Author(s):

Achiraya Jiraprasertwong ◽

Pattaratorn Karnchanapaisal ◽

Kessara Seneesrisakul ◽

Pramoch Rangsunvigit ◽

Sumaeth Chavadej

Keyword(s):

Upflow Anaerobic Sludge Blanket ◽

Anaerobic Sludge ◽

Two Phase ◽

Operational Conditions ◽

Anaerobic Sludge Blanket

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Effects of Monoculture, Crop Rotation, and Soil Moisture Content on Selected Soil Physicochemical and Microbial Parameters in Wheat Fields

Applied and Environmental Soil Science ◽

10.1155/2012/593623 ◽

2012 ◽

Vol 2012 ◽

pp. 1-13 ◽

Cited By ~ 7

Author(s):

A. Marais ◽

M. Hardy ◽

M. Booyse ◽

A. Botha

Keyword(s):

Soil Moisture ◽

Microbial Communities ◽

Crop Rotation ◽

Management Practices ◽

Agricultural Soils ◽

Soil Microbial Communities ◽

Soil Microbial Activity ◽

Most Probable Number ◽

Probable Number ◽

Soil Microbial

Different plants are known to have different soil microbial communities associated with them. Agricultural management practices such as fertiliser and pesticide addition, crop rotation, and grazing animals can lead to different microbial communities in the associated agricultural soils. Soil dilution plates, most-probable-number (MPN), community level physiological profiling (CLPP), and buried slide technique as well as some measured soil physicochemical parameters were used to determine changes during the growing season in the ecosystem profile in wheat fields subjected to wheat monoculture or wheat in annual rotation with medic/clover pasture. Statistical analyses showed that soil moisture had an over-riding effect on seasonal fluctuations in soil physicochemical and microbial populations. While within season soil microbial activity could be differentiated between wheat fields under rotational and monoculture management, these differences were not significant.

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Microbial Removal of Pb(II) Using an Upflow Anaerobic Sludge Blanket (UASB) Reactor

Catalysts ◽

10.3390/catal11040512 ◽

2021 ◽

Vol 11 (4) ◽

pp. 512

Author(s):

Jeremiah Chimhundi ◽

Carla Hörstmann ◽

Evans M. N. Chirwa ◽

Hendrik G. Brink

Keyword(s):

Treatment Process ◽

Removal Rate ◽

Upflow Anaerobic Sludge Blanket ◽

Uasb Reactor ◽

Anaerobic Sludge ◽

Reduction Potentials ◽

Oxidation Reduction ◽

Anaerobic Sludge Blanket ◽

Microbial Removal ◽

Maximum Removal

The main objective of this study was to achieve the continuous biorecovery and bioreduction of Pb(II) using an industrially obtained consortia as a biocatalyst. An upflow anaerobic sludge blanket reactor was used in the treatment process. The bioremediation technique that was applied made use of a yeast extract as the microbial substrate and Pb(NO3)2 as the source of Pb(II). The UASB reactor exhibited removal efficiencies of between 90 and 100% for the inlet Pb concentrations from 80 to 2000 ppm and a maximum removal rate of 1948.4 mg/(L·d) was measured. XRD and XPS analyses of the precipitate revealed the presence of Pb0, PbO, PbS and PbSO4. Supporting experimental work carried out included growth measurements, pH, oxidation–reduction potentials and nitrate levels.

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