scholarly journals Enterococci and Bacilli from surface water: assessment of their resistance to copper and antibiotics

2020 ◽  
Vol 43 ◽  
pp. e49854
Author(s):  
Luciana Furlaneto Maia ◽  
Gabriela Batista Gomes Bravo ◽  
Alex Kiyomassa Watanabe ◽  
Nayara de Oliveira Batista ◽  
Márcia Cristina Furlaneto
Keyword(s):  
Heavy Metal ◽  
Inhibitory Concentration ◽  
Metal Removal ◽  
Resistant Bacteria ◽  
Copper Metal ◽  
Enterococcus Casseliflavus ◽  
Potential Applications ◽  
Tolerance To Copper ◽  
Water Assessment ◽  
Potential Tolerance

Heavy metal-resistant bacteria can be efficient bioremediators of metals and might provide an alternative method for metal removal in contaminated environments. The present study aims to isolate bacteria from the aquatic environment and evaluate their potential tolerance to copper metal, aiming at bioremediation processes. Also, compare co-resistance to heavy metal and antibiotics. The morphology of isolates was observed, and sequence analysis (16S ribosomal DNA) revealed that isolated strains were closely related to species belonging to the genera Enterococcus and Bacillus. Bacterial isolates were resistant to CuSO4, with a minimum inhibitory concentration of 0.78 mg ml-1. Enterococcus lactis was resistant to a combination of copper and tetracycline. The other tested isolates were sensitive to the tested antimicrobials. The metal removal ability of these isolates was assayed using atomic absorption spectroscopy, and the strains 27, 23, and E. lactis were best at removing heavy metals, at 87.7%. Enterococcus casseliflavus EC55 was 62%, followed by Bacillus aerius (18.4%), E. casseliflavus EC70 (10%) and Bacillus licheniformis (10%). Based on our findings, Enterococcus sp and Bacillus sp. have potential applications in enhanced remediation of contaminated environments.

Roles of different humin and heavy-metal resistant bacteria from composting on heavy metal removal

2020 ◽  
Vol 296 ◽  
pp. 122375 ◽  
Author(s):  
Yuquan Wei ◽  
Yue Zhao ◽  
Xinyu Zhao ◽  
Xintong Gao ◽  
Yansi Zheng ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Removal ◽  
Heavy Metal Removal ◽  
Resistant Bacteria ◽  
Heavy Metal Resistant

EPS production and bioremoval of heavy metals by mixed and pure bacterial cultures isolated from Ankara Stream

2015 ◽  
Vol 72 (9) ◽  
pp. 1488-1494 ◽  
Author(s):  
Nur Koçberber Kiliç ◽  
Güliz Kürkçü ◽  
Durna Kumruoğlu ◽  
Gönül Dönmez
Keyword(s):  
Heavy Metal ◽  
Metal Removal ◽  
Heavy Metal Removal ◽  
Mixed Cultures ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Bacterial Cultures ◽  
Removal Capacity ◽  
Pure Cultures ◽  
Pure Bacterial Cultures

This study is focused on isolation of Ni(II), Cu(II) and Cr(VI) resistant bacteria to assess their exopolysaccharide (EPS) production and related bioremoval capacities. Mixed cultures had higher heavy metal removal capacity in media with molasses (MAS) than the control cultures lacking this carbon (AS) containing 50 mg/l of heavy metal. The yields were 32%, 75.7%, and 51.1% in MAS, while the corresponding values were 29%, 55.1%, and 34.5% in AS, respectively. Purification of the strains 1, 5 and 6 present in the mixed cultures decreased the bioremoval capacities of the mixed culture samples, although these strains produced higher EPS amounts in MAS agar. Strain 5 had the highest Cu(II) (69.1%) and Cr(VI) (43.1%) removal rates at 25 mg/l initial concentration of each pollutant with EPS amounts of 0.74 g/l and 1.05 g/l, respectively. This strain was identified as Stenotrophomonas maltophilia. The presented data show that especially mixed and also pure cultures of bacterial strains isolated from Ankara Stream could be assessed as potential bioremoval agents in the treatment of Cu(II) or Cr(VI) containing wastewaters.

MILKING ANTISEPTICS AGAINST BACTERIA MULTIDRUG-RESISTANT BOVINE MASTITIS

2021 ◽  
Vol 11 (2) ◽  
pp. 646-651
Author(s):  
Patricia Nascente ◽  
PEDRO RASSIER DOS SANTOS ◽  
HELENICE GONZALEZ DE LIMA ◽  
SILVIA DE OLIVEIRA HUBNER ◽  
PATRICIA DA SILVA NASCENTE
Keyword(s):  
Sodium Hypochlorite ◽  
Inhibitory Concentration ◽  
Bovine Mastitis ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Minimal Bactericidal Concentration ◽  
Cytotoxicity Test ◽  
Enterococcus Casseliflavus ◽  
Antiseptic Solutions

This work aims to verify the in vitro action of antiseptics used in the pre- and post-dipping against multidrug-resistant bacteria from bovine mastitis. Antiseptic solutions in the concentration of use of iodine, chlorhexidine and sodium hypochlorite were tested against Staphylococcus aureus (n=12), Staphylococcus sciuri (n=1), Staphylococcus lentus (n=1), Streptococcus sp. (n=1), Enterococcus faecalis (n=1), Enterococcus casseliflavus (n=1), Kocuria kristinae (n=2), Kocuria varians (n=1). Was verified the Minimal Inhibitory and Bactericidal Concentration. The cytotoxicity test complemented the work. It was found that the Minimal Inhibitory Concentration and Minimal Bactericidal Concentration of the iodine, sodium hypochlorite and chlorhexidine was effective in the values recommended for use, however the presence of organic material reduced the activity of the tested products. Chlorhexidine showed higher efficiency of the other disinfectants.

scholarly journals Heavy Metal Adsorption Using Magnetic Nanoparticles for Water Purification: A Critical Review

Materials ◽  
2021 ◽  
Vol 14 (24) ◽  
pp. 7500
Author(s):  
Christos Liosis ◽  
Athina Papadopoulou ◽  
Evangelos Karvelas ◽  
Theodoros E. Karakasidis ◽  
Ioannis E. Sarris
Keyword(s):  
Heavy Metal ◽  
Magnetic Nanoparticles ◽  
Adsorption Capacity ◽  
Water Purification ◽  
Metal Ion ◽  
Metal Removal ◽  
Heavy Metal Removal ◽  
Desorption Process ◽  
Potential Applications ◽  
Modified Magnetic Nanoparticles

Research on contamination of groundwater and drinking water is of major importance. Due to the rapid and significant progress in the last decade in nanotechnology and its potential applications to water purification, such as adsorption of heavy metal ion from contaminated water, a wide number of articles have been published. An evaluating frame of the main findings of recent research on heavy metal removal using magnetic nanoparticles, with emphasis on water quality and method applicability, is presented. A large number of articles have been studied with a focus on the synthesis and characterization procedures for bare and modified magnetic nanoparticles as well as on their adsorption capacity and the corresponding desorption process of the methods are presented. The present review analysis shows that the experimental procedures demonstrate high adsorption capacity for pollutants from aquatic solutions. Moreover, reuse of the employed nanoparticles up to five times leads to an efficiency up to 90%. We must mention also that in some rare occasions, nanoparticles have been reused up to 22 times.

scholarly journals Heavy metal tolerance in indigenous bacteria isolated from the industrial sewage in Kemisan River, Tangerang, Banten, Indonesia

2017 ◽  
Vol 18 (4) ◽  
pp. 1481-1486 ◽  
Author(s):  
WAHYU IRAWATI ◽  
SEMUEL RIAK ◽  
NIDA SOPIAH ◽  
SUSI SULISTIA
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Metal Removal ◽  
Metal Tolerance ◽  
Heavy Metal Removal ◽  
Heavy Metal Tolerance ◽  
Indigenous Bacteria ◽  
Industrial Sewage

Irawati W, Riak S, Sopiah N, Sulistia S. 2017. Heavy metal tolerance in indigenous bacteria isolated from the industrial sewage in Kemisan River, Tangerang, Banten, Indonesia. Biodiversitas 18: 1481-1486. The bacterial study is a part of human calling in preserving the earth. Many indigenous bacteria isolated from heavy metal contaminated sites had resistance to heavy metal toxicity and could be used for heavy metal removal. The aims of this study were to isolate heavy metal-tolerant indigenous bacteria from the industrial sewage of Kemisan River in Tangerang, Banten, Indonesia. The potency of bacterial isolates to remove heavy metals was also determined. The heavy-metal tolerance was determined by measuring the minimum inhibitory concentration. The potency of bacterial isolate for removing heavy metals from the medium was determined by an atomic absorption spectrophotometer. The results showed that there were eight heavy metal-resistant bacteria isolated from Kemisan River with minimum inhibitory concentration ranging from 7 mM to 11 mM. Isolate PbSI1 was the highest lead tolerant bacteria, and also tolerant to copper and zinc. The isolate was able to remove 91.25% lead, 73.38% zinc, and 98.57% copper from medium supplemented with the mixture of these heavy metals. The addition of 9 mM of lead in the medium affected the morphological appearance of isolate colonies i.e PbSI1 and PbSI3 to become darker which might occur due to the survival mechanism of bacteria by absorbing the lead inside the cells. The finding of this study indicated that isolate PbSI1 was a promising bacterium, which could be further developed for heavy metal removal.

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