Controlled Release of rhBMP-2 Loaded Poly(DL-lactic-co-glycolic Acid)/ Calcium Phosphate Cement Composites In Vivo

2006 ◽  
pp. 973-976
Author(s):  
P.Q. Ruhé ◽  
O.C. Boerman ◽  
F.G.M. Russel ◽  
P.H.M. Spauwen ◽  
Antonious G. Mikos ◽  
...  
Keyword(s):  
Controlled Release ◽  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Glycolic Acid ◽  
Cement Composites

Controlled release of rhBMP-2 loaded poly(dl-lactic-co-glycolic acid)/calcium phosphate cement composites in vivo

2005 ◽  
Vol 106 (1-2) ◽  
pp. 162-171 ◽  
Author(s):  
P.Q. Ruhé ◽  
O.C. Boerman ◽  
F.G.M. Russel ◽  
P.H.M. Spauwen ◽  
A.G. Mikos ◽  
...  
Keyword(s):  
Controlled Release ◽  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Glycolic Acid ◽  
Cement Composites

Controlled Release of rhBMP-2 Loaded Poly(DL-lactic-co-glycolic Acid)/ Calcium Phosphate Cement Composites In Vivo

2006 ◽  
Vol 309-311 ◽  
pp. 973-976
Author(s):  
P.Q. Ruhé ◽  
O.C. Boerman ◽  
F.G.M. Russel ◽  
P.H.M. Spauwen ◽  
Antonious G. Mikos ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Glycolic Acid ◽  
Slow Release ◽  
Release Kinetics ◽  
Bone Morphogenetic Protein 2 ◽  
Cement Composites ◽  
Plga Microparticles

The release kinetics of recombinant human bone morphogenetic protein-2 (rhBMP-2) loaded poly(DL-lactic-co-glycolic acid)/calcium phosphate cement (PLGA/Ca-P cement) composites were studied in vivo. RhBMP-2 was radiolabeled with 131I and entrapped within PLGA microparticles or adsorbed onto the microparticle surface. PLGA microparticles were prepared of high molecular weight (HMW) PLGA (weight average molecular weight [Mw] 49,100 ± 1,700) or low molecular weight (LMW) PLGA (Mw 5,900 ± 300) and used for preparation of 30:70 wt% PLGA/Ca-P cement composite discs. Release of 131I-rhBMP-2 loaded composites was assessed by scintigraphic imaging according to a 22 two-level full factorial design in the rat ectopic model during four weeks. In vivo release kinetics varied among formulations. All formulations showed slow release without initial burst, and displayed a linear release from 3 to 28 days. Release of LMW entrapped rhBMP-2 composites (1.7 ± 0.3%/day) was significantly faster than release from other formulations (p < 0.01). After 28 days, retention within the composites was 65 ± 5%, 75 ± 4%, 50 ± 4% and 70 ± 6% of the initial rhBMP-2 for HMW entrapped, HMW adsorbed, LMW entrapped and LMW adsorbed rhBMP-2 composites, respectively. Release from the composite was probably slowed down by an interaction of rhBMP-2 and Ca-P cement after rhBMP-2 release from PLGA microparticles. We conclude that PLGA/Ca-P cement composites can be considered as sustained slow release vehicles and that the release and retention of rhBMP-2 can be modified according to the desired profile to a limited extent.

scholarly journals Polymeric-Calcium Phosphate Cement Composites-Material Properties:In VitroandIn VivoInvestigations

2010 ◽  
Vol 2010 ◽  
pp. 1-14 ◽  
Author(s):  
Rania M. Khashaba ◽  
Mervet M. Moussa ◽  
Donald J. Mettenburg ◽  
Frederick A. Rueggeberg ◽  
Norman B. Chutkan ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Setting Time ◽  
Cement Composites ◽  
Tetracalcium Phosphate ◽  
Methyl Vinyl Ether ◽  
Orthopedic Applications ◽  
Hydroxyapatite Cement

New polymeric calcium phosphate cement composites (CPCs) were developed. Cement powder consisting of 60 wt% tetracalcium phosphate, 30 wt% dicalcium phosphate dihydrate, and 10 wt% tricalcium phosphate was combined with either 35% w/w poly methyl vinyl ether maleic acid or polyacrylic acid to obtain CPC-1 and CPC-2. The setting time and compressive and diametral tensile strength of the CPCs were evaluated and compared with that of a commercial hydroxyapatite cement.In vitrocytotoxicity andin vivobiocompatibility of the two CPCs and hydroxyapatite cement were assessed. The setting time of the cements was 5–15 min. CPC-1 and CPC-2 showed significantly higher compressive and diametral strength values compared to hydroxyapatite cement. CPC-1 and CPC-2 were equivalent to Teflon controls after 1 week. CPC-1, CPC-2, and hydroxyapatite cement elicited a moderate to intense inflammatory reaction at 7 days which decreased over time. CPC-1 and CPC-2 show promise for orthopedic applications.

RHBMP-2 RELEASE FROM INJECTABLE POLY(DL-LACTIC-CO-GLYCOLIC ACID)/CALCIUM-PHOSPHATE CEMENT COMPOSITES

2003 ◽  
Vol 85 ◽  
pp. 75-81 ◽  
Author(s):  
QUINTEN P. RUHE ◽  
ELIZABETH L. HEDBERG ◽  
NESTOR TORIO PADRON ◽  
PAUL H.M. SPAUWEN ◽  
JOHN A. JANSEN ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Glycolic Acid ◽  
Cement Composites

Biocompatibility and degradation of poly(DL-lactic-co-glycolic acid)/calcium phosphate cement composites

2005 ◽  
Vol 74A (4) ◽  
pp. 533-544 ◽  
Author(s):  
P. Quinten Ruhé ◽  
Elizabeth L. Hedberg ◽  
Nestor Torio Padron ◽  
Paul H. M. Spauwen ◽  
John A. Jansen ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Glycolic Acid ◽  
Cement Composites

scholarly journals A bone replacement-type calcium phosphate cement that becomes more porous in vivo by incorporating a degradable polymer

2021 ◽  
Vol 32 (7) ◽  
Author(s):  
Akiyoshi Shimatani ◽  
Hiromitsu Toyoda ◽  
Kumi Orita ◽  
Yuta Ibara ◽  
Yoshiyuki Yokogawa ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Alginic Acid ◽  
Setting Time ◽  
Zealand White Rabbit ◽  
Control Group ◽  
Degradable Polymer ◽  
Low Viscosity ◽  
Bone Replacement

AbstractThis study investigated whether mixing low viscosity alginic acid with calcium phosphate cement (CPC) causes interconnected porosity in the CPC and enhances bone replacement by improving the biological interactions. Furthermore, we hypothesized that low viscosity alginic acid would shorten the setting time of CPC and improve its strength. CPC samples were prepared with 0, 5, 10, and 20% low viscosity alginic acid. After immersion in acetate buffer, possible porosification in CPC was monitored in vitro using scanning electron microscopy (SEM), and the setting times and compressive strengths were measured. In vivo study was conducted by placing CPC in a hole created on the femur of New Zealand white rabbit. Microcomputed tomography and histological examination were performed 6 weeks after implantation. SEM images confirmed that alginic acid enhanced the porosity of CPC compared to the control, and the setting time and compressive strength also improved. When incorporating a maximum amount of alginic acid, the new bone mass was significantly higher than the control group (P = 0.0153). These biological responses are promising for the translation of these biomaterials and their commercialization for clinic applications.

In Vivo Bone Tissue Formation Induced by Caclium Phosphate Paste Composite with Demineralized Bone Matrix

2007 ◽  
Vol 330-332 ◽  
pp. 1091-1094
Author(s):  
H. Kim ◽  
M. Park ◽  
Su Young Lee ◽  
Kang Yong Lee ◽  
Hyun Min Kim ◽  
...  
Keyword(s):  
Animal Model ◽  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Marker Gene ◽  
Tissue Formation ◽  
Bone Tissue Formation ◽  
Demineralized Bone

Demineralized bone matrix (DBM)-calcium phosphate cement (CPC) composites were subjected to cellular test of osteogenic potentials and implantation in animal model. The expression of osteogenic marker gene from mouse preosteoblast cell line MC3T3-E1 adhered to the DBM-CPC composite was much higher than plain CPC. In addition, the DBM-CPC composite implanted nude mice revealed osteoinduction between the implanted composite and adjacent tissues, whereas the plain CPC induced osteoconduction.

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