Effect of Substrate Concentration on Hydrogen Production from Fermentation of Sugar Wastewater

2013 ◽  
Vol 409-410 ◽  
pp. 235-241
Author(s):  
Zheng Zhou ◽  
Kai Liu ◽  
Qiu Yang He
Keyword(s):  
Hydrogen Production ◽  
Production Rate ◽  
Substrate Concentration ◽  
Anaerobic Fermentation ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Gas Production ◽  
Production Performance ◽  
Volatile Acid ◽  
Hydrogen Production Rate

The biological hydrogen production by the sugar wastewater is an effective way to achieve the reclamation. In this paper, the effect of substrate concentration on the hydrogen production is discussed through employing the self-made continuous flow anaerobic fermentation hydrogen production reactor, taking the sludge in urban sewage treatment plant as the inoculated sludge and the simulated sugar wastewater as the substrate. The experimental results show that the best hydrogen production effect can be obtained when the temperature is (37±1) °C, HRT is 7h, the water alkalinity is around 530mg/L and the substrate concentration is 5000mg/L, namely the organic load is 60kgCOD/(m3·d). The volumes of gas production and hydrogen production both reach the maximum. The average values are respectively 36.2L/d and 21.8L/d. The obtained hydrogen production rate is 0.93kgCOD/(m3·d). During the whole process, the proportion of volatile acid composition remains stable, which is the butyric acid-type fermentation. When the concentration of COD is increased to 6000-8000mg/L, the ability of hydrogen production of system will be significantly dropped due to the increase of pH of system. The hydrogen production performance can be restored through artificially and timely lowering the water alkalinity. However, the hydrogen production rate will be decreased compared to the previous situation.

Operation control of anaerobic digesters on the basis of enzyme activity tests

2009 ◽  
Vol 60 (4) ◽  
pp. 957-964 ◽  
Author(s):  
Levente Kardos ◽  
György Palkó ◽  
József Oláh ◽  
Katalin Barkács ◽  
Gyula Záray
Keyword(s):  
Enzyme Activity ◽  
Acid Concentration ◽  
Pilot Plant ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Gas Production ◽  
Full Scale ◽  
Volatile Acid ◽  
Control Parameters ◽  
Activity Measurements

In our experimental work the pilot plant and full scale anaerobic bioreactors of a communal sewage treatment plant were tested by applying usual control parameters (pH, volatile acid content, alkalinity, gas composition), and enzyme activity (dehydrogenase, protease, lipase) measurements. Influence of temperature change was examined in pilot plant scale, while the effect of alteration in specific organic matter load both in pilot and full scale. Among the control parameters only the change of the volatile acid concentration reflected the occurred influences. During the temperature varying experimental phase the dehydrogenase enzyme activity excellently indicated the influence of the different conditions. The effect of altering substrate load onto the gas production was also well followed by the enzyme activity data (mainly protease, lipase), and more rapidly than by measuring volatile acid concentration. In practice it is expedient to use enzyme activity measurements in those cases, when changes in the substrate composition and load are frequent. Another advantage of these tests is that they can be carried out quickly and at a relative low cost.

Hydrogen Production from Cornstalk with Different Pretreatment Methods by Anaerobic Fermentation

2013 ◽  
Vol 777 ◽  
pp. 173-177
Author(s):  
Xin Yuan Liu ◽  
Ru Ying Li ◽  
Min Ji ◽  
Di Liu ◽  
Yan Ming Cai
Keyword(s):  
Energy Consumption ◽  
Hydrogen Production ◽  
Production Rate ◽  
Lignocellulosic Biomass ◽  
Anaerobic Fermentation ◽  
Economic Effect ◽  
Biohydrogen Production ◽  
Hydrogen Yield ◽  
Hydrogen Production Rate ◽  
Pretreatment Method

As lignocellulosic biomass, the cornstalk should be pretreated before anaerobic fermentation for hydrogen production. In this study, HCl, NaOH and enzyme were employed for cornstalk pretreatment and the products were used for anaerobic biohydrogen production. Hydrogen yield and hydrogen production rate were investigated to optimize cornstalk pretreatment method. In addition, the economic effect and energy consumption were also considered to evaluate the pretreatment methods. The optimum cornstalk pretreatment method was soaking in 2% NaOH at 50°C for 48h with a hydrogen yield of 55.0 ml/g-TS and a hydrogen production rate of 6.5 ml/h/g-VS in anaerobic hydrogen production.

High yield hydrogen production in a single-chamber membrane-less microbial electrolysis cell

2010 ◽  
Vol 61 (3) ◽  
pp. 721-727 ◽  
Author(s):  
Yejie Ye ◽  
Liyong Wang ◽  
Yingwen Chen ◽  
Shemin Zhu ◽  
Shubao Shen
Keyword(s):  
Hydrogen Production ◽  
Production Rate ◽  
Hydrogen Concentration ◽  
Current Density ◽  
Production Performance ◽  
Electrolysis Cell ◽  
Hydrogen Production Rate ◽  
High Hydrogen ◽  
Single Chamber ◽  
Hydrogen Recovery

The single-chamber membrane-less MEC exerted much better hydrogen production performance while given higher applied voltages than it did at lower. High applied voltages that could shorten the reaction time and the exposure of anode to air for at least 30 min between cycles can significantly suppress methanogen and increase hydrogen production. At an applied voltage of 1.0 V, a hydrogen production rate of 1.02 m3/m3/day with a current density of 5.7 A/m2 was achieved. Cathodic hydrogen recovery and coulombic efficiency were 63.4% and 69.3% respectively. The hydrogen concentration of mixture gas produced of 98.4% was obtained at 1.0 V, which was the best result of reports. The reasons that such a high hydrogen concentration can be achieved were probably the high electrochemical activity and hydrogen production capability of the active microorganisms. Increase in substrate concentrations could not improve MEC's performance, but increased the reaction times. Further, reactor configuration and operation factors optimisation should be considered to increase current density, hydrogen production rate and hydrogen recovery.

Improvement of Organic Loading Rate on Hydrogen Production Using Continuous Stirred Tank

2013 ◽  
Vol 805-806 ◽  
pp. 1382-1386
Author(s):  
Jian Hui Zhao ◽  
Ning Li ◽  
Yong Feng Li
Keyword(s):  
Hydrogen Production ◽  
Production Rate ◽  
Gas Production ◽  
Stirred Tank ◽  
Organic Loading Rate ◽  
Organic Loading ◽  
Hydrogen Production Rate ◽  
Brown Sugar ◽  
High Hydrogen ◽  
Continuous Stirred Tank

The influence of organic loading rates (OLRs) on the production of fermentation hydrogen was investigated in a continuous stirred tank reactor (CSTR) with brown sugar water as the fermentation substrate, and sewage sludge as the initiation of reaction. Six OLRs were examined, ranging from 12 kg/m3·d to 32 kg/m3·d. The biogas and hydrogen production rates continuously increased with increasing OLR (12 kg/m3·d to 32 kg/m3·d).It reached a maximum production rate of 18.6L/d and a hydrogen production rate of 6.4L/d at OLR= 32 kg/m3·d. Compared with the initial 12kg/m3·d, gas production improved by 89% and 87%, respectively. During system operation, the reactor could maintain a high hydrogen production rate of ethanol-type fermentation by adding a certain amount of NaOH in the reactor to regulate the pH level.

scholarly journals Study on the hydrogen production ability of high-efficiency bacteria and synergistic fermentation of maize straw by a combination of strains

RSC Advances ◽  
2019 ◽  
Vol 9 (16) ◽  
pp. 9030-9040
Author(s):  
Hongxu Bao ◽  
Xin Zhang ◽  
Hongzhi Su ◽  
Liangyu Li ◽  
Zhizhong Lv ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Production Rate ◽  
Substrate Concentration ◽  
High Efficiency ◽  
Hydrogen Production Rate ◽  
Culture Time ◽  
Maize Straw ◽  
Initial Ph

B2 + X9 was inoculated at the same time, and 6% were inoculated in a ratio of 1 : 1. At an initial pH of 6, the substrate concentration was 12 g L−1, the culture time was 40 h, and the hydrogen production rate of the combined strain was 12.6 mmol g−1.

scholarly journals Enhanced hydrogen production of Rhodobacter sphaeroides promoted by extracellular H+ of Halobacterium salinarum

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Jiang-Yu Ye ◽  
Yue Pan ◽  
Yong Wang ◽  
Yi-Chao Wang
Keyword(s):  
Hydrogen Production ◽  
Production Rate ◽  
Rhodobacter Sphaeroides ◽  
Coupled System ◽  
Purple Membrane ◽  
Halobacterium Salinarum ◽  
Control Group ◽  
Hydrogen Production Rate ◽  
Production Of Hydrogen ◽  
The Impact

Abstract Purpose This study utilized the principle that the bacteriorhodopsin (BR) produced by Halobacterium salinarum could increase the hydrogen production of Rhodobacter sphaeroides. H. salinarum are co-cultured with R. sphaeroides to determine the impact of purple membrane fragments (PM) on R. sphaeroides and improve its hydrogen production capacity. Methods In this study, low-salinity in 14 % NaCl domesticates H salinarum. Then, 0–160 nmol of different concentration gradient groups of bacteriorhodopsin (BR) and R. sphaeroides was co-cultivated, and the hydrogen production and pH are measured; then, R. sphaeroides and immobilized BR of different concentrations are used to produce hydrogen to detect the amount of hydrogen. Two-chamber microbial hydrogen production system with proton exchange membrane-assisted proton flow was established, and the system was operated. As additional electricity added under 0.3 V, the hydrogen production rate increased with voltages in the coupled system. Results H salinarum can still grow well after low salt in 14% NaCl domestication. When the BR concentration is 80 nmol, the highest hydrogen production reached 217 mL per hour. Both immobilized PC (packed cells) and immobilized PM (purple membrane) of H. salinarum could promote hydrogen production of R. sphaeroides to some extent. The highest production of hydrogen was obtained by the coupled system with 40 nmol BR of immobilized PC, which increased from 127 to 232 mL, and the maximum H2 production rate was 18.2 mL−1 h−1 L culture. In the 192 h experiment time, when the potential is 0.3 V, the hydrogen production amount can reach 920 mL, which is 50.3% higher than the control group. Conclusions The stability of the system greatly improved after PC was immobilized, and the time for hydrogen production of R. sphaeroides significantly extended on same condition. As additional electricity added under 0.3 V, the hydrogen production rate increased with voltages in the coupled system. These results are helpful to build a hydrogen production-coupled system by nitrogenase of R. sphaeroides and proton pump of H. salinarum. Graphical abstract

Effects of applied voltage on hydrogen production rate of a single reactor BML with Clostridium sp.

2015 ◽  
Vol 98 ◽  
pp. 383-389 ◽  
Author(s):  
Chiung-Yi Cheng ◽  
Kuang-Li Cheng ◽  
Terng-Jou Wan ◽  
Wei-Nung Kuo ◽  
Feng-Jen Chu ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Production Rate ◽  
Applied Voltage ◽  
Hydrogen Production Rate
Sign in / Sign up

Export Citation Format

Share Document