Correlation between Anthocyanins, Total Phenolics Content and Antioxidant Activity of Purple Corn (Zea mays L.)

2014 ◽  
Vol 651-653 ◽  
pp. 220-226
Author(s):  
Min Zhu ◽  
Zhen Sheng Shi ◽  
Feng Hai Li
Keyword(s):  
Superoxide Anion ◽  
Anion Radical ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Superoxide Anion Radical ◽  
Scavenging Activity ◽  
Purple Corn

Six purple corn grains were chosen to investigate their antioxidant activities and the relationships between anthocyanins and phenolics contents with antioxidant activities. Purple grains were screened for their antioxidant potentials using various in-vitro models such as superoxide anion radical scavenging activity and reducing power at various concentrations. The result showed that ZS39 and FS11 exhibited the highest anthocyanins and total phenolics contents. ZS39 and FS11 also exhibited the highest superoxide anion radical scavenging activity. There was no significant difference among the reducing power of six different purple corn grain extracts. It was found that there were positive correlations between anthocyanins and superoxide anion radical scavenging activity or reducing power, R2=0.9911, R2=0.9873, respectively. Whereas the correlations between total phenolics and superoxide anion radical scavenging activity or reducing power, R2=0.9863, R2=0.9826, respectively. It suggested that purple corn appeared to be a rich and interesting source of natural antioxidants.

Anti-Oxidant Activity of Anthocyanins of Black Soybean In Vitro

2013 ◽  
Vol 634-638 ◽  
pp. 1423-1428 ◽  
Author(s):  
Yan Song ◽  
Guo Ping Yu ◽  
Chao Ran Dou ◽  
Hao Wu ◽  
Zhu Gong
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Superoxide Anion Radical ◽  
Scavenging Activity ◽  
Antioxidant Power ◽  
Black Soybean

The aim of the work was to research antioxidant activity of the anthoyanins extracts of black soybean. The whole soybean through defatted, were to determine the phenolic and anthocyanin contents in black soybean extracts and to examine antioxidant activity of extract against DPPH and ABTS assays, and Ferric Reducing Antioxidant Power (FRAP), and superoxide anion radical scavenging activity. The defatted sample was extracted using ethanol by ultrasonic. The optimum DPPH and ABTS radical scavenging capacity (%) were 68.34% and 70.12%, VC was 59.76% and 95.23%, respectively, and contains high levels of reducing power and superoxide anion radical scavenging activity. The present results indicate that the anthoyanins extract from black soybean has greater anti-oxidative and is useful in functional food and other applications.

scholarly journals BIOMEDICAL POTENTIAL OF SELECTED MUSHROOM SPECIES

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Tatjana Stanojković ◽  
Marijana Kosanić
Keyword(s):  
Free Radical ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Superoxide Anion Radical ◽  
Total Phenol Content ◽  
Scavenging Activity

The aim of this study was to determine neuroprotective, antioxidant, antimicrobial and cytotoxic potential of acetone extracts of Coprinus comatus and Coprinopsis picacea mushrooms. The neuroprotetive activity was tested against acetylcholinesterase enzyme using the Ellman method. Antioxidant activity was evaluated by free radical scavenging ability, superoxide anion radical scavenging activity and reducing power. The total phenol content was examined using Folin-Ciocalteu reagent. The antimicrobial potential was determined by a microdilution method against 12 microorganisms. The cytotoxic activity was tested using MTT method on the Hela, A549 and LS174 cells. Our results indicate that C. comatus expressed a stronger neuroprotective effect (the percentage of inhibition of acetylcholinesterase was within the range 19.66-51.73%) than C. picacea. In antioxidant effect C. comatus had more potent free radical scavenging activity (IC50 = 276.69 μg/mL) and superoxide anion radical scavenging activity (IC50 = 39.40 μg/mL), while reducing power was relatively similar for both species. The total amount of phenols for C. comatus and C. picacea was 50.57 and 50.20 μg PE/mg of dry extracts, respectively. In antimicrobial activity, C. picacea showed a better effect with MIC values from 0.1 to 7.5 mg/mL. Finally, C. picacea expressed stronger cytotoxicity toward A549 and LS174 cells, while C. comatus was more active against Hela cell.

scholarly journals Callus formation, phenolics content and related antioxidant activities in tissue culture of a medicinal plant colocynth (Citrullus colocynthis)

2021 ◽  
Vol 10 (2) ◽  
pp. 79-94
Author(s):  
Farouk K. El-Baz ◽  
Amal A. Mohamed ◽  
Sami I. Ali
Keyword(s):  
Total Phenolics ◽  
Antioxidant Activities ◽  
Callus Formation ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Callus Cultures ◽  
Scavenging Activity ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Callus cultures from stems, leaves and roots of colocynth were initiated on MS media supplemented with various combinations of 2,4 dichlorophenoxyacetic acid (2,4-D) with kinetin (KIN) and benzyladenine (BA) with α-naphthaleneacetic acid (NAA). The highest percentage of callus formation frequency (98.9%) was obtained from stem explants grown on MS media supplemented with (1.0 mg/L) 2,4-D + (1.0 mg/L) KIN. The total phenolics and flavonoid content of the colocynth callus cultures were measured. The results showed that the MS medium supplemented with 6.0 mg/L 2,4-D + 2.0 mg/L KIN (MD3) gave the highest content of total phenolics (19.2 mg/100g d.w.) in leaf-derived calli. The highest content of flavonoids (47.3 mg/100g d.w.) was obtained in stem derived calli grown on the same medium (MD3). Antioxidant activities of extracts were determined using different assays, including DPPH radical scavenging activity, hydrogen peroxide (H2O2) scavenging activity and ferric reducing power. Leaf-derived calli cultured on MS medium + 2.0 mg/L 2,4-D + 1.0 mg/L KIN (MD1) showed the highest DPPH radical scavenging activity (85.3%). The highest percentage of H2O2 scavenging activity (61.4%) was detected in leaf explant-derived calli growing on MD1. The leaf-derived calli growing on (MD3) gave the highest ferric reducing power (22.3 μg/g d.w.), compared to the activities of stems, leaves and roots of in vitro grown seedlings (3.28, 12.9 and 2.85 μg/g d.w.), which were used as controls. On the basis of the current findings, we conclude that MS media supplemented with different combinations of 2,4-D and KIN yields higher phenolics, flavonoids contents and antioxidant activities than MS media supplemented with BA and NAA.

scholarly journals Superoxide anion radical scavenging activity of bilberry (Vaccinium myrtillus L.)

2010 ◽  
Vol 1 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Vesna Tumbas ◽  
Jasna Čanadanović-Brunet ◽  
Lars Gille ◽  
Sonja Đilas ◽  
Gordana Ćetković
Keyword(s):  
Superoxide Anion ◽  
Anion Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Vaccinium Myrtillus ◽  
Superoxide Anion Radical ◽  
Scavenging Activity ◽  
Vaccinium Myrtillus L

scholarly journals The presence of thecagpathogenicity island is associated with increased superoxide anion radical scavenging activity byHelicobacter pylori

2005 ◽  
Vol 44 (2) ◽  
pp. 227-232 ◽  
Author(s):  
Jacob J. Briedé ◽  
Raymond G.J. Pot ◽  
Ernst J. Kuipers ◽  
Arnoud H.M. Vliet ◽  
Jos C.S. Kleinjans ◽  
...  
Keyword(s):  
Superoxide Anion ◽  
Anion Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Superoxide Anion Radical ◽  
Scavenging Activity

Optimization of Pyrogallol Autoxidation Conditions and Its Application in Evaluation of Superoxide Anion Radical Scavenging Capacity for Four Antioxidants

2016 ◽  
Vol 99 (2) ◽  
pp. 504-511 ◽  
Author(s):  
Qing-An Zhang ◽  
Xi Wang ◽  
Yun Song ◽  
Xue-Hui Fan ◽  
Juan Francisco García Martín
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Gallic Acid ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Superoxide Anion Radical ◽  
Scavenging Activity ◽  
Pyrogallol Autoxidation

Abstract In this study, some factors influencing pyrogallol autoxidation, including EDTA, temperature, and solvent, were systematically investigated to improve its feasibility in the evaluation of antioxidants for the first time. Subsequently, the improved pyrogallol autoxidation conditions were used to assess the superoxide anion scavenging activity (SASA) of four commonly used antioxidants, namely, ascorbic acid, rutin, catechin, and gallic acid, by both the reaction rate method and the terminated method. The results indicate that pyrogallol autoxidation could be successfully used to determine the antioxidant capacity of ascorbic acid and rutin, which correspondingly suggests the feasibility of its use to measure the superoxide anion radical scavenging activity of polysaccharides and flavonols, because these compounds have a similar basic structural unit as ascorbic acid and rutin, respectively. Unexpectedly, however, pyrogallol autoxidation cannot be used to evaluate the SASA of catechin and gallic acid, although their good antioxidant capacity was confirmed by the 1,1-diphenyl-2-picrylhydrazyl assay. Together, these results suggest the importance of noting the conditions used for pyrogallol autoxidation when assessing the SASA of targeted compounds.

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