Biohydrogen Fermentation with a Continuous Stirred Tank Reactor Containing Immobilized Anaerobic Sludge

2010 ◽  
Vol 113-116 ◽  
pp. 1986-1989
Author(s):  
Wei Han ◽  
Zhan Qing Wang ◽  
Yan Wang ◽  
Yong Feng Li ◽  
Chuan Ping Yang
Keyword(s):  
Activated Carbon ◽  
Hydrogen Production ◽  
Anaerobic Sludge ◽  
Hydrogen Energy ◽  
Hydrogen Yield ◽  
Continuous Stirred Tank Reactor ◽  
Fermentation Products ◽  
Immobilization Technology ◽  
Continuous Stirred Tank ◽  
Renewable Technology

Anaerobic hydrogen fermentation converting organic wastes to clean hydrogen energy is a renewable technology for hydrogen production. This study developed a technology to immobilize hydrogen producing bacteria on granular activated carbon. It was investigated that the effects of organic loading impact on immobilized hydrogen production system. It was found that hydrogen production yield increased from 2.85 L to 5.2 L as COD increased from 2000 mg/L to 6000 mg/L. The liquid fermentation products were ethanol and acetic acid with percentage of 48.97% and 40.26%, respectively. VSS increased from 4555 to 5450 mg/L which results in the increasing of hydrogen yield. Therefore, immobilized anaerobic sludge by grunular activated carbon could be a promising immobilization technology for biohydrogen fermentation.

Control of Start-up and Operation in Continuous Fermentation of Bio-Hydrogen Production Reactor

2011 ◽  
Vol 183-185 ◽  
pp. 552-556
Author(s):  
Zhi Qin ◽  
Dan Qin ◽  
Dan Li
Keyword(s):  
Acetic Acid ◽  
Hydrogen Production ◽  
Butyric Acid ◽  
Ph Value ◽  
Continuous Fermentation ◽  
Hydrogen Yield ◽  
Continuous Stirred Tank Reactor ◽  
Fermentation Products ◽  
Biogas Yield ◽  
Acid Type

Bio-hydrogen production from diluted molasses by anaerobic activated sludge was investigated in a continuous stirred-tank reactor (CSTR) under condition of continuous flow in this study. Research shows that the reactor started up under the condition of influent COD concentration 3000mg/L, HRT8h, pH6.5~7.5 and (35±1) °C. The process performed steadily and a dominant butyric acid and acetic acid type fermentation population was established, acetic acid and butyric acid accounted for about 80% in the liquid fermentation products. The effluent PH value was maintained about 5.0. The biogas yield could reach at 4.87L/d while hydrogen yield reached 41.25mL/d under the condition. When influent COD concentration rose to 5500 mg/L, the biogas yield and hydrogen yield as high as 9.45L/d and 119.98mL/d were obtained.

The Substrate Concentration Effects on Biohydrogen Production in Continuous Stirred Tank Reactor

2010 ◽  
Vol 113-116 ◽  
pp. 2062-2066
Author(s):  
Wei Han ◽  
Hong Chen ◽  
Jing Wei Zhang ◽  
Jian Yu Yang ◽  
Yong Feng Li ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Substrate Concentration ◽  
H2 Production ◽  
Stirred Tank ◽  
Cellular Activity ◽  
Continuous Stirred Tank Reactor ◽  
Concentration Effects ◽  
Fermentation Products ◽  
Tank Reactor ◽  
Continuous Stirred Tank

Substrate concentration effects on H2 production performance in a continuous stirred-tank reactor (CSTR) were investigated. Using molasses as the feeding, the CSTR system was operated at chemical oxygen demand (COD) of 2000-8000 mg/L to identify the optimal working substrate concentration. Increasing substrate concentration (2000~6000 mg/L) gave better biomass content and hydrogen production, signifying that the average cellular activity for H2 production may be enhanced as the substrate concentration increased. The overall maximal biogas and hydrogen production yield were 18.69 L and 6.01 L, respectively, both of them occurred at 6000 mg/L. The gas phase H2 content did not vary considerably regardless of changes in substrate concentration. This reflects that the CSTR was a relatively stable H2-producing system. The major soluble products from hydrogen fermentation were ethanol and acetic acid, accounting for 59% and 23% of total liquid fermentation products, respectively. Thus, the dominant H2 producers in the mixed culture belonged to acidogenic bacteria that underwent ethanol-type fermentation. However, the biomass content and hydrogen production yield tended to decrease as the substrate concentration increased to 8000 mg/L, suggesting that granular sludge formation and cellular activity for H2 production may be inhibited at high substrate concentration. Ethanol, acetic, butyric and propionic were the main liquid fermentation products with the percentages of 31%, 24%, 20% and 18%, which formed the mixed-type fermentation.

Characterization of Bio-Hydrogen Production from Carbohydrates with Bacteria Ethanoligenens R3

2010 ◽  
Vol 113-116 ◽  
pp. 1884-1889
Author(s):  
An Ying Jiao ◽  
Yong Feng Li ◽  
Kun Liu ◽  
Bing Liu
Keyword(s):  
Hydrogen Production ◽  
Culture Medium ◽  
Previous Experiment ◽  
Hydrogen Yield ◽  
Continuous Stirred Tank Reactor ◽  
Brown Sugar ◽  
Biogas Yield ◽  
Initial Ph ◽  
Continuous Stirred Tank

An ethanol-based hydrogen producing bacteria Ethanoligenens R3 was isolated from continuous stirred tank reactor used for hydrogen production in previous experiment. Batch culture experiments were operated in this study with the fermentative temperature of 35°C and substrate concentration of 10g/L. The performance of hydrogen production from glucose, brown sugar and starch by Ethanoligenens R3 was investigated. The results show that the optimal substrate used for bio-hydrogen production was glucose with the maximum hydrogen yield of 834 mlH2/L culture. Furthermore, effect of initial pH of the culture medium on hydrogen production was studied. It is demonstrated that Ethanoligenens R3 reached the maximum biogas yield of 1760 ml/L culture with the maximum total VFAs concentration of 3329.39 mg/L at the initial pH of culture medium of 5.5.

Influence of activated carbon on performance and microbial communities in the treatment of solvent pollutant mixtures in a continuous stirred tank reactor

2020 ◽  
Vol 6 (5) ◽  
pp. 1445-1455
Author(s):  
Pablo Ferrero ◽  
Marta Izquierdo ◽  
Francisco Javier Álvarez-Hornos ◽  
Josep Manuel Penya-Roja ◽  
Vicente Martínez-Soria
Keyword(s):  
Activated Carbon ◽  
Microbial Communities ◽  
Granular Activated Carbon ◽  
Stirred Tank ◽  
Continuous Stirred Tank Reactor ◽  
Carbon Addition ◽  
Stirred Tank Reactor ◽  
Tank Reactor ◽  
Continuous Stirred Tank ◽  
Pollutant Mixtures

Granular activated carbon addition could promote specific microorganisms which favour the anaerobic removal of non-readily biodegradable solvent compounds through their influence on the degradation (methanogenesis) of intermediates.

Rapid Start-Up of Ethanol-Type Fermentation in Biological Hydrogen Production Reactor from Molasses Wastewater

2010 ◽  
Vol 113-116 ◽  
pp. 623-631
Author(s):  
Li Ran Yue ◽  
Yong Feng Li ◽  
Wei Han ◽  
Jing Li Xu ◽  
Hong Chen ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Ph Value ◽  
Anaerobic Fermentation ◽  
Oxygen Demand ◽  
Low Ph ◽  
Continuous Stirred Tank Reactor ◽  
Fermentation Products ◽  
Biological Hydrogen Production ◽  
Molasses Wastewater ◽  
Start Up

Research on anaerobic fermentation biohydrogen production from molasses wastewater in a continuous stirred-tank reactor (CSTR) was conducted. Emphasis was focused on the rapid start-up of ethanol-type fermentation in biological hydrogen production reactor. It was found that an initial biomass of 17.71 g/L, temperature of 35°C±1°C, hydraulic retention time (HRT) of 6 h, the reactor could start-up the ethanol-type fermentation at the range of 2000-4000 mg/L and at pH from 3.23 to 4.39 in 12 days with COD (chemical oxygen demand), respectively. The content of hydrogen was 45.77% in the fermentation biogas and the COD removal was 8%. As the hydrogen production system experienced low pH (3.23-4.0), the ethanol-produce bacterial can resume easier compared with other fermentation bacteria which are difficult to restore. In addition, when the pH value ranged from 4.0 to 4.63, the hydrogen production increased with the content of ethanol in liquid fermentation products increased. However, it was detected that the yield of hydrogen decreased with a high content of ethanol in the pH value ranged from 3.23 to 4.0, which demonstrated pH value played the most important role on hydrogen production within low pH.

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