Characterization of Bio-Hydrogen Production from Carbohydrates with Bacteria Ethanoligenens R3

2010 ◽  
Vol 113-116 ◽  
pp. 1884-1889
Author(s):  
An Ying Jiao ◽  
Yong Feng Li ◽  
Kun Liu ◽  
Bing Liu
Keyword(s):  
Hydrogen Production ◽  
Culture Medium ◽  
Previous Experiment ◽  
Hydrogen Yield ◽  
Continuous Stirred Tank Reactor ◽  
Brown Sugar ◽  
Biogas Yield ◽  
Initial Ph ◽  
Continuous Stirred Tank

An ethanol-based hydrogen producing bacteria Ethanoligenens R3 was isolated from continuous stirred tank reactor used for hydrogen production in previous experiment. Batch culture experiments were operated in this study with the fermentative temperature of 35°C and substrate concentration of 10g/L. The performance of hydrogen production from glucose, brown sugar and starch by Ethanoligenens R3 was investigated. The results show that the optimal substrate used for bio-hydrogen production was glucose with the maximum hydrogen yield of 834 mlH2/L culture. Furthermore, effect of initial pH of the culture medium on hydrogen production was studied. It is demonstrated that Ethanoligenens R3 reached the maximum biogas yield of 1760 ml/L culture with the maximum total VFAs concentration of 3329.39 mg/L at the initial pH of culture medium of 5.5.

Control of Start-up and Operation in Continuous Fermentation of Bio-Hydrogen Production Reactor

2011 ◽  
Vol 183-185 ◽  
pp. 552-556
Author(s):  
Zhi Qin ◽  
Dan Qin ◽  
Dan Li
Keyword(s):  
Acetic Acid ◽  
Hydrogen Production ◽  
Butyric Acid ◽  
Ph Value ◽  
Continuous Fermentation ◽  
Hydrogen Yield ◽  
Continuous Stirred Tank Reactor ◽  
Fermentation Products ◽  
Biogas Yield ◽  
Acid Type

Bio-hydrogen production from diluted molasses by anaerobic activated sludge was investigated in a continuous stirred-tank reactor (CSTR) under condition of continuous flow in this study. Research shows that the reactor started up under the condition of influent COD concentration 3000mg/L, HRT8h, pH6.5~7.5 and (35±1) °C. The process performed steadily and a dominant butyric acid and acetic acid type fermentation population was established, acetic acid and butyric acid accounted for about 80% in the liquid fermentation products. The effluent PH value was maintained about 5.0. The biogas yield could reach at 4.87L/d while hydrogen yield reached 41.25mL/d under the condition. When influent COD concentration rose to 5500 mg/L, the biogas yield and hydrogen yield as high as 9.45L/d and 119.98mL/d were obtained.

Biohydrogen Fermentation with a Continuous Stirred Tank Reactor Containing Immobilized Anaerobic Sludge

2010 ◽  
Vol 113-116 ◽  
pp. 1986-1989
Author(s):  
Wei Han ◽  
Zhan Qing Wang ◽  
Yan Wang ◽  
Yong Feng Li ◽  
Chuan Ping Yang
Keyword(s):  
Activated Carbon ◽  
Hydrogen Production ◽  
Anaerobic Sludge ◽  
Hydrogen Energy ◽  
Hydrogen Yield ◽  
Continuous Stirred Tank Reactor ◽  
Fermentation Products ◽  
Immobilization Technology ◽  
Continuous Stirred Tank ◽  
Renewable Technology

Anaerobic hydrogen fermentation converting organic wastes to clean hydrogen energy is a renewable technology for hydrogen production. This study developed a technology to immobilize hydrogen producing bacteria on granular activated carbon. It was investigated that the effects of organic loading impact on immobilized hydrogen production system. It was found that hydrogen production yield increased from 2.85 L to 5.2 L as COD increased from 2000 mg/L to 6000 mg/L. The liquid fermentation products were ethanol and acetic acid with percentage of 48.97% and 40.26%, respectively. VSS increased from 4555 to 5450 mg/L which results in the increasing of hydrogen yield. Therefore, immobilized anaerobic sludge by grunular activated carbon could be a promising immobilization technology for biohydrogen fermentation.

Effect of Organic Loading Rate on Fermentative Hydrogen Production in CSTR

2010 ◽  
Vol 156-157 ◽  
pp. 732-736
Author(s):  
Shuang Gao ◽  
Bing Wang ◽  
Lei Lei Zhu ◽  
Wei Han ◽  
Hong Chen ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Hydrogen Production ◽  
Acid Concentration ◽  
Statistical Correlation ◽  
Organic Loading Rate ◽  
Hydrogen Yield ◽  
Continuous Stirred Tank Reactor ◽  
Organic Loading ◽  
Biogas Yield ◽  
The Impact

This study investigated the impact of five organic loading rates (OLRs) ranging from 16 kg COD/m3-d to 32 kg COD/m3-d on the performance of a continuous stirred tank reactor (CSTR) for biological hydrogen production, with molasses as substrate. Hydraulic Retention Time (HRT) was kept at 6 hours and the temperature 35°C. Oxidation-reduction potential (ORP) varied from -328 mV to -419 mV. The ethanol-type fermentation has been conducted during the process. A positive statistical correlation was observed between OLR and biogas yield; however, it became negative for the OLR of 32 kg COD/m3-d (Fig1). The system maintained the highest average biogas and hydrogen yield of 14.66 L/d and 5.17 L/d at OLR of 28 kg COD/m3-d. Compared with the first stage (16 kg COD/m3-d), ethanol and butyric concentration of the last stage (32 kg COD/m3-d) increased about 2.01 and 3.79 times, respectively; Acetic acid concentration surged around 200 mg/L; Prop ionic acid concentration dropped slightly; Valeric acid concentration, which took a small portion in the volatile fatty acid (VFA), kept low. When OLR was kept at 28 kg COD/m3-d, the average ethanol to acetic acid ratio is 3.90.Thus proved that the optimal OLR for hydrogen production under experimental condition is 28 kg COD/m3-d.

Characteristics of Biohydrogen Production by Ethanoligenens Sp. R3 Isolated from Continuous Stirred Tank Reactor

2010 ◽  
Vol 152-153 ◽  
pp. 702-707
Author(s):  
An Ying Jiao ◽  
Yong Feng Li ◽  
Bing Liu ◽  
Kun Liu ◽  
Jing Li Xu
Keyword(s):  
Carbon Source ◽  
Hydrogen Production ◽  
Nitrogen Source ◽  
Ph Value ◽  
Stirred Tank ◽  
Continuous Stirred Tank Reactor ◽  
Stirred Tank Reactor ◽  
Tank Reactor ◽  
Initial Ph ◽  
Continuous Stirred Tank

Ethanoligenens sp. R3 isolated from anaerobic sludge in a continuous stirred tank reactor was investigated for its characteristics of fermentative hydrogen production in this study. Effects of initial pH value, fermentation temperature and proportion of carbon/nitrogen source on hydrogen production were investigated in batch experiments. The typical substrate for hydrogen production of glucose and peptone were used as the carbon source and nitrogen source, respectively. The results show that the maximum hydrogen production yield of 834 mlH2/L culture was obtained with initial pH value of 5.5 and fermentation temperature at 35°C. In addition, the maximum average hydrogen production rate of 10.87mmolH2/g cell dry weighth was achieved at the carbon/nitrogen source ratio of 3.3. The degradation efficiency of the glucose used as the carbon source ranged from 91.5% to 95.43% during the conversion of glucose to hydrogen by bacteria Ethanoligenens sp. R3.

scholarly journals Anaerobic treatment of glycerol for methane and hydrogen production

2013 ◽  
Vol 14 (2) ◽  
pp. 149-156 ◽  
Keyword(s):  
Hydrogen Production ◽  
Ph Value ◽  
Anaerobic Treatment ◽  
Microbial Culture ◽  
Batch Reactors ◽  
Continuous Stirred Tank Reactor ◽  
Organic Loading ◽  
Loading Rates ◽  
Fermentative Hydrogen Production ◽  
Initial Ph

This work focused on glycerol exploitation for biogas and hydrogen production. Anaerobic digestion of pure glycerol was studied in a continuous stirred tank reactor (CSTR), operated under mesophilic conditions (35oC) at various organic loading rates. The overall operation of the reactor showed that it could not withstand organic loading rates above 0.25 g COD L-1 d-1, where the maximum biogas (0.42 ± 0.05 L (g COD)-1) and methane (0.30 ± 0.04 L (g COD)-1) production were achieved. Fermentative hydrogen production was carried out in batch reactors under mesophilic conditions (35oC), using heat-pretreated anaerobic microbial culture as inoculum. The effects of initial concentration of glycerol and initial pH value on hydrogen production were studied. The highest yield obtained was 22.14 ± 0.46 mL H2 (g COD added)-1 for an initial pH of 6.5 and an initial glycerol concentration of 8.3 g COD L-1. The main metabolic product was 1.3 propanediol (PDO), while butyric and acetic acids as well as ethanol, at lower concentrations, were also determined.

The Study on Anaerobic Fermentative Biological Hydrogen Production with Organic Wastes

2010 ◽  
Vol 113-116 ◽  
pp. 1476-1480
Author(s):  
Xiao Ye Liu ◽  
Yi Sun ◽  
Jian Yu Yang ◽  
Yong Feng Li
Keyword(s):  
Ph Value ◽  
Biogas Production ◽  
Removal Rate ◽  
Maximum Yield ◽  
Liquid Product ◽  
Oxygen Demand ◽  
H2 Production ◽  
Hydrogen Yield ◽  
Continuous Stirred Tank Reactor ◽  
Brown Sugar

This papre discussed the ability of H2-production and wastewater treatment, a continuous stirred tank reactor (CSTR)using a synthesized substrate with brown sugar wastewater was conducted to investigate the hydrogen yield, fermentation type of biohydrogen production, and the chemical oxygen demand (COD) removal rate, respectively. The results show that when the pH value was 4.0～4.5, OLR was 24.5kg/(m3•d), oxidation-reduction potential was -350～-450mv, temperature was 34.0°C～36.0°C, hydraulic retention time was 6h～8h, the maximum yield of biogas production reached 20L/d and the maximum content of hydrogen is 70%. Detection of the end liquid product, ethanol and acetic acid are main, they are 70% ~90% of the total liquid product, so that is called ethanol-type fermentation.

scholarly journals Production and Biochemical Characterization of a High Maltotetraose (G4) Producing Amylase fromPseudomonas stutzeriAS22

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Hana Maalej ◽  
Hanen Ben Ayed ◽  
Olfa Ghorbel-Bellaaj ◽  
Moncef Nasri ◽  
Noomen Hmidet
Keyword(s):  
Culture Medium ◽  
Biochemical Characterization ◽  
Amylase Activity ◽  
Potato Starch ◽  
Pseudomonas Stutzeri ◽  
Crude Enzyme Preparation ◽  
Amylase Production ◽  
Initial Ph ◽  
Very High

Amylase production and biochemical characterization of the crude enzyme preparation fromPseudomonas stutzeriAS22 were evaluated. The highestα-amylase production was achieved after 24 hours of incubation in a culture medium containing 10 g/L potato starch and 5 g/L yeast extract, with initial pH 8.0 at 30°C under continuous agitation at 200 rpm. The optimum temperature and pH for the crudeα-amylase activity were 60°C and 8.0, respectively. The effect of different salts was evaluated and it was found that bothα-amylase production and activity were Ca2+-dependent. The amylolytic preparation was found to catalyze exceptionally the formation of very high levels of maltotetraose from starch (98%, w/w) in the complete absence of glucose since the initial stages of starch hydrolysis (15 min) and hence would have a potential application in the manufacturing of maltotetraose syrups.

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