Interface between Nanophotonics and Biotechnology: How the Near-Field Can Boost Proteomics Based on LSPR Nano Sensor

2010 ◽  
Vol 139-141 ◽  
pp. 1554-1557 ◽  
Author(s):  
Xi Xi Huang ◽  
Zhong Cao ◽  
Yong Le Liu ◽  
Yi Min Dai ◽  
Ju Lan Zeng ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Low Cost ◽  
Near Field ◽  
Visible Region ◽  
High Sensitivity ◽  
Nano Particles ◽  
Localized Surface Plasmon ◽  
Bioanalytical Application

An novel optical nano biosensor based on gold capped nano-particles for detecting binding events between ligands and receptor molecules as well as interactions among proteins without use of labels has been presented in this paper. The optical properties of nano-sized gold particles exhibiting pronounced adsorption in the visible region which called as localized surface plasmon resonance (LSPR) have been exploited, whose peak wavelengths depended exquisitely on the refractive index of the surrounding. In comparison with surface plasmon resonance (SPR) technology, the optical nano biosensor possessed high sensitivity, surprisingly low “bulk effect”, ease of preparation, and low-cost polymer based fabrication, which opened a promising bioanalytical application in practice.

Localized surface plasmon resonance shift and its application in scanning near-field optical microscopy

2021 ◽  
Author(s):  
Jiawei Zhang ◽  
Gitanjali Kolhatkar ◽  
Andreas Ruediger
Keyword(s):  
Raman Spectroscopy ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Near Field ◽  
Localized Surface Plasmon ◽  
Resonance Shift ◽  
Raman Modes ◽  
Tip Enhanced Raman Spectroscopy

The localized surface plasmon resonance (LSPR) position in tip-enhanced Raman spectroscopy (TERS) is of great importance to the understanding and interpretation of the relative intensity of different enhanced Raman modes....

scholarly journals A Methodology for Porcine Circovirus 2 (PCV-2) Quantification Based on Gold Nanoparticles

Materials ◽  
2020 ◽  
Vol 13 (5) ◽  
pp. 1087
Author(s):  
Caroline R. Basso ◽  
Taís F. Cruz ◽  
Bruna L. Silva ◽  
Valber A. Pedrosa ◽  
João P. Araújo Junior
Keyword(s):  
Gold Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Low Cost ◽  
Porcine Circovirus ◽  
Localized Surface Plasmon ◽  
Transmission Electron ◽  
Porcine Circovirus 2 ◽  
Assembled Monolayers

The aim of the current study is to introduce a methodology aimed at producing a biosensor that uses gold nanoparticles (AuNPs) to detect porcine circovirus 2 (PCV-2). This biosensor was based on AuNPs, which were modified with self-assembled monolayers (SAMs) and antibodies. The AuNPs’ surface and virus modification process applied to enable antibody binding was accompanied by localized surface plasmon resonance (LSPR), surface plasmon resonance (SPR), transmission electron microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDX). Virus quantification was possible by the light absorption difference in the spectrum at concentrations of 105, 106, 107, 108, and 109 DNA copies/mL PCV-2 in relation to quantitative PCR (qPCR), with an R2 value >0.98. The visualization of colorimetric changes in the different PCV-2 concentrations was possible without the use of equipment. The biosensor production methodology presented reproducibility and specificity, as well as easy synthesis and low cost. An enhanced version of it may be used in the future to replace traditional tests such as PCR.

scholarly journals Fabrication of Interconnected Plasmonic Spherical Silver Nanoparticles with Enhanced Localized Surface Plasmon Resonance (LSPR) Peaks Using Quince Leaf Extract Solution

Nanomaterials ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1557 ◽  
Author(s):  
Shujahadeen B. Aziz ◽  
Govar Hussein ◽  
M. A. Brza ◽  
Sewara J. Mohammed ◽  
R. T. Abdulwahid ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Low Cost ◽  
Ftir Spectrum ◽  
Localized Surface Plasmon ◽  
Ag Nps ◽  
Extract Solution

Interconnected spherical metallic silver nanoparticles (Ag NPs) were synthesized in the current study using a green chemistry method. The reduction of silver ions to Ag NPs was carried out with low-cost and eco-friendly quince leaves. For the first time, it was confirmed that the extract solution of quince leaves could be used to perform green production of Ag NPs. Fourier transform infrared spectroscopy (FTIR) was conducted to identify the potential biomolecules that were involved in the Ag NPs. The results depicted that the biosynthesis of Ag NPs through the extract solution of quince leaf was a low-cost, clean, and safe method, which did not make use of any contaminated element and hence, had no undesirable effects. The majority of the peaks in the FTIR spectrum of quince leaf extracts also emerged in the FTIR spectrum of Ag NPs but they were found to be of less severe intensity. The silver ion reduction was elaborated in detail on the basis of the FTIR outcomes. In addition, through X-ray diffraction (XRD) analysis, the Ag NPs were also confirmed to be crystalline in type, owing to the appearance of distinct peaks related to the Ag NPs. The creation of Ag NPs was furthermore confirmed by using absorption spectrum, in which a localized surface plasmon resonance (LSPR) peak at 480 nm was observed. The LSPR peak achieved in the present work was found to be of great interest compared to those reported in literature. Field emission scanning electron microscopy (FESEM) images were used to provide the morphology and grain size of Ag NPs. It was shown from the FESEM images that the Ag NPs had interconnected spherical morphology.

scholarly journals Low-Cost Localized Surface Plasmon Resonance Biosensing Platform with a Response Enhancement for Protein Detection

Nanomaterials ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 1019 ◽  
Author(s):  
Yun Liu ◽  
Ning Zhang ◽  
Ping Li ◽  
Li Yu ◽  
Shimeng Chen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Low Cost ◽  
Microfluidic Channel ◽  
Protein Detection ◽  
Localized Surface Plasmon

There are many potential applications for biosensors that can provide real-time analysis, such as environmental monitoring and disease prevention. In this study, we investigated a simple strategy for real-time protein detection, which had the advantages of affordability, fast response, portability, and ease of use. A robust quantification of protein interaction was achieved by combining capillary localized surface plasmon resonance (LSPR) sensors and complementary metal–oxide–semiconductor (CMOS) image sensors. Gold nanoparticles were modified on the inner wall of the capillary, which was used as a microfluidic channel and sensing surface. We functionalized one of the LSPR sensors using ligand bound to gold nanoparticle. Our proposed biosensing platform could be easily multiplexed to achieve high throughput screening of biomolecular interactions, and it has the potential for use in disposable sensors. Moreover, the sensing signal was enhanced by the extinction effect of gold nanoparticles. The experimental results showed that our device could achieve qualitative identification and quantitative measurement of transferrin and immunoglobulin G (IgG). As a field-portable and low-cost optical platform, the proposed LSPR biosensing device is broadly applicable to various protein binding tests via a similar self-assembly of organic ultrathin films.

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