GC-MS Analysis of Chemical Composition and Free Radical Scavenging Activity of Elaeagnus angustifolia Bark

2011 ◽  
Vol 183-185 ◽  
pp. 854-858 ◽  
Author(s):  
Chuan Ling Si ◽  
Pan Pan Qin ◽  
Yi Yuan Lu ◽  
Lei Wu ◽  
Hai Hua Wang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Chemical Composition ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Elaeagnus Angustifolia ◽  
Ic50 Values ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay ◽  
Soluble Fractions

Elaeagnus angustifolia L., a hardwood species in the family of Elaeagnaceae, has long been used as part of traditional medicines to treat various diseases. However, limited study is available on the chemical composition and antioxidant activity of E. angustifolia bark. In this study, we undertook a phytochemical investigation of E. angustifolia bark by gas chromatographic and mass spectrometric (GC-MS) analysis. And antioxidant activity of the crude and following soluble fractions of E. angustifolia bark was evaluated by DPPH free radical scavenging assay. GC-MS investigation of 95% EtOH crude extracts from E. angustifolia bark detected 40 compounds, and DPPH free radical scavenging assay indicated that the bark EtOAc and BuOH soluble fractions exhibited significant antioxidant potential (IC50 values of 12.1 and 12.4 µg/ml, respectively) comparing with α-tocopherol and BHT (IC50 values 12.3 and 13.8 µg/ml, respectively), which were used as positive controls.

scholarly journals Isoprenylated Xanthone and Benzophenone Constituents of the Pericarp of Garcinia planchonii

2014 ◽  
Vol 9 (12) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Duong Hoang Trinh ◽  
Ly Dieu Ha ◽  
Phuong Thu Tran ◽  
Lien-Hoa Dieu Nguyen
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Hexane Extract ◽  
Spectroscopic Methods ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

A new xanthone, planchoxanthone (1), together with six known compounds, garcinianone A (2), cowanin (3), rubraxanthone (4), β-mangostin (5), dulcisxanthone B (6), and guttiferone Q (7), were isolated from an n-hexane extract of the pericap of Garcinia planchonii. Their structures were elucidated using spectroscopic methods. Antioxidant activity of the isolated compounds was tested using the DPPH free radical scavenging assay.

scholarly journals In –vitro Antioxidant Activity of Methanolic Extract of the Roots of Bergenia ciliata

2018 ◽  
Vol 1 (4) ◽  
pp. 31-34
Author(s):  
Suman Lal Shrestha ◽  
Suresh Awale ◽  
Surya Kant Kalauni
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Ic50 Value ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay ◽  
In Vitro Antioxidant Activity

Bergenia ciliata is an essential medicinal plant used in regions where western medicines are inaccessible due to their unavailability and high cost. The methanolic extract of Bergenia ciliataroots was separated for phytochemical elements and in-vitro antioxidant activity. The plant extract showed the rich outgrowth of secondary metabolites that play the role for biological activities. The higher antioxidant functioning of the plant is due to the occurrence of reactive elements like phenols and flavonoids. The antioxidant functioning of the plant extract was measured by DPPH free radical scavenging assay. In DPPH free radical scavenging assay the IC50 value of Bergenia ciliata was found to be 11.21μg/mL, while the IC50 value of standard ascorbic acid was found to be 45.93μg/mL

Total Phenolic Content and In vitro Antioxidant Activity of Endophytic Fungi Isolated from Calotropis procera L.

2019 ◽  
Vol 15 (2) ◽  
pp. 232-241 ◽  
Author(s):  
Reena Rani ◽  
Dushyant Sharma ◽  
Monika Chaturvedi ◽  
Jaya Parkash Yadav
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Endophytic Fungi ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Calotropis Procera ◽  
Total Phenolic ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

Background: Endophytic fungi are microorganisms that colonize inside the plant tissues without causing any disease symptoms. Endophytic fungi isolated from medicinal plants have emerged as an interesting source for the isolation of bioactive compounds. In this study, we selected Calotropis procera, a member of the Asclepiadaceae family commonly called ‘Aak’ to evaluate the antioxidant potential of isolated endophytic fungi. </P><P> Objective: The aim of this study was to assess the total phenolic content (TPC), antioxidant capacity by using different assay and phytochemical screening of endophytic fungi isolated from Calotropis procera (leaves, stem and root). </P><P> Method: Crude ethyl acetate extracts of 20 different endophytic fungi isolated from Calotropis procera were tested for their preliminary phytoconstituents presence, TPC estimation (by Folin–Ciocalteu colorimetric assay) and antioxidant potential [1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, hydrogen peroxide (H2O2) free radical scavenging assay, β-carotene-linoleic free radical scavenging assay, metal chelating assay and xanthine oxidase inhibitory assay]. Results: Phenols, carbohydrates, saponins, tannins, alkaloids and flavanoids were the main phytoconstituents present in the endophytic fungi. Among the endophytes, Aspergillus nomius showed the highest TPC [72.71±1.67 µg GAE (gallic acid equivalent) /mg dry weight of fungi] and antioxidant activity for DPPH free radical scavenging assay (68.86±0.19%). A high positive linear correlation was found between TPC and xanthine oxidase inhibitory assay (R2-0.890) and between TPC and DPPH free radical scavenging assay (R2-0.839). Aspergillus and Fusarium genus species showed significant antioxidant activity by the different assay. Conclusion: The present study revealed some endophytic fungi from Calotropis procera could be a potential source of novel natural antioxidant compounds.

scholarly journals COMPARATIVE EVALUATION OF ANTIOXIDANT ACTIVITY OF SUBSTITUTED FLAVONES

2017 ◽  
Vol 10 (11) ◽  
pp. 288
Author(s):  
Helen William ◽  
Narayanan N
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Dpph Assay ◽  
Superoxide Free Radical ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

  Objective: The flavonoids are a heterogeneous group of plant polyphenols that are endowed with several biological activities including antioxidant, anti-inflammatory, immunomodulatory, antiviral, antimutagenic, and anticarcinogenic properties. They are believed to interfere with the various free radical-producing systems and they also enhance the functions of endogenous antioxidants. The aim of this study was to compare the antioxidant activity of synthesized substituted flavones by various free radical scavenging assays.Methods: The flavones used in the study, 6,3’,4’ - trihydroxy flavones (THF) and 3-hydroxy-6,3’-dimethoxy flavones were synthesized using standard procedures and their antioxidant activity was compared by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, hydrogen peroxide scavenging assay, hydroxyl radical scavenging assay, reducing power capacity, superoxide free radical scavenging assay, and 2,2′-azino-bis-3-ethylbenzthiazoline-6- sulphonic acid assay.Results: In the DPPH assay, THF has an inhibitory concentration 50% value of 18.89 μg/ml which was much better than that of standard ascorbic acid which was 26.1 μg/ml. The THF had a much better antioxidant activity in most of the free radical scavenging assays.Conclusion: From the study, it can be seen that the flavones showed significant antioxidant activity that can be used for treatment of various diseases

scholarly journals Synthesis and Antioxidant Activity of Some Novel Benzimidazole Derivatives

2018 ◽  
Vol 16 (2) ◽  
pp. 245-249 ◽  
Author(s):  
Nilima Karmaker ◽  
Dilshad Noor Lira ◽  
Biplab Kumar Das ◽  
Uttom Kumar ◽  
Abu Shara Shamsur Rouf
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Spectroscopic Data ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Benzimidazole Derivatives ◽  
Ic50 Value ◽  
Substituted Benzaldehydes ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

A series of 2-substituted-5-methylbenzimidazole derivatives (3a-e) were synthesized by reacting   4-methyl-1,2-phenylenediamine (1) with a number of p-substituted benzaldehydes (2a-e) in moderate yields (25.51-   40.21%). The synthesized compounds (3a-e) were characterized by spectroscopic data and were evaluated for   antioxidant activity using DPPH free radical scavenging assay. The compounds showed significant antioxidant   activity having IC50 value of 1.054-19.05 μg/ml as compared to the standard BHT (26.96 μg/ml).Dhaka Univ. J. Pharm. Sci. 16(2): 245-249, 2017 (December)

scholarly journals Antioxidant activities of traditional plants in Sri Lanka by DPPH free radical-scavenging assay

Data in Brief ◽  
2018 ◽  
Vol 17 ◽  
pp. 870-875 ◽  
Author(s):  
Kotaro Hara ◽  
Takao Someya ◽  
Katsura Sano ◽  
Yoshimasa Sagane ◽  
Toshihiro Watanabe ◽  
...  
Keyword(s):  
Free Radical ◽  
Sri Lanka ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay

In Vitro Regeneration and Free Radical Scavenging Assay of Hypericum perforatum L.

2018 ◽  
Vol 42 (2) ◽  
pp. 161-167 ◽  
Author(s):  
Mohammad Yaseen Mir ◽  
Azra N. Kamili ◽  
Qazi P. Hassan ◽  
Sabreena Rafi ◽  
Javid A. Parray ◽  
...  
Keyword(s):  
Free Radical ◽  
Hypericum Perforatum ◽  
In Vitro Regeneration ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Hypericum Perforatum L ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay
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