Molecularly Imprinted Polymer for Preparation and Adsorption of Phenylalanine

Molecular imprinting technology was employed to produce one kind of Phenylalanine ( Phe) molecularly imprinted polymers (MIP)by precipitation polymerization using Phe, anhydrous alcohol, acrylamide, ethylene glycol dimethacrylate, and azobisisobutyronitrile as template, porogen, functional monomer, cross-linker and initiator respectively. In this study, the polymerization conditions were optimized. The template on particle size and morphology of polymers were investigated in detail by scanning electron microscopy (SEM) and adsorption isotherm determination. The binding properties of Phe on imprinted polymers were evaluated in water by equilibrium rebinding experiments, and the maximum number of adsorption was 0.60 mmol/g. It is indicated that the existence of binding sites in imprinted polymers was proved and the binding sites showed good specific and selective capability to the template molecule Phe. So the polymers would be used to separate Phe from medicine and food.

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Study of Chromatographic Properties of Catecholamines and their Acidic Metabolites Using Novel Molecularly Imprinted Polymers as Stationary Phases

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.903.15 ◽

2021 ◽

Vol 903 ◽

pp. 15-21

Author(s):

Antons Podjava ◽

Artūrs Šilaks

Keyword(s):

Molecularly Imprinted Polymers ◽

Binding Sites ◽

Stationary Phases ◽

Adrenal Tumors ◽

Binding Properties ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Simultaneous Extraction ◽

Synthetic Procedure ◽

Molecularly Imprinted Sorbents

Quantitative analysis of catecholamines and their acidic metabolites can provide vital information for diagnosis and treatment of various diseases (such as adrenal tumors). However, complicated purification protocols are usually required to isolate the analytes. Molecularly imprinted polymers (MIPs) have attracted considerable attention as a method of selectively separating desired compounds from their matrix. Therefore, we propose a synthetic procedure for new molecularly imprinted sorbents that possess selective binding sites for both catecholamines and their acidic metabolites. The new polymer utilizes non-covalent and semi-covalent imprinting methods. The binding properties of MIPs were evaluated using chromatographic experiments. The results suggest that the MIPs bind with catecholamines much more selectively than with their acidic metabolites. Therefore, increasing sorption selectivity of the acids would make it possible to perform simultaneous extraction of both compound groups.

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Sythesis of Molecularly Imprinted Polymers for the Binding and Recognition of Nonylphenol

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.641-642.55 ◽

2013 ◽

Vol 641-642 ◽

pp. 55-59 ◽

Cited By ~ 1

Author(s):

Yan Jie Bi ◽

Wen Yan Li ◽

Chen Yuan ◽

Bin Song Wang

Keyword(s):

Molecularly Imprinted Polymers ◽

High Selectivity ◽

Sol Gel ◽

Template Molecule ◽

Binding Properties ◽

Adsorption Selectivity ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Selective Enrichment ◽

Sol Gel Process

In this paper, molecularly imprinted polymers with high selectivity for nonylphenol (NP) were synthesized by sol-gel process using 4-vinylpyridine (4-Vpy) as functional monomer, ethyleneglycol dimethacrylate as crosslinker (EGDMA), azobisisobutyronitrile (AIBN) as initiator, NP as template molecules. The performance of imprinted material was evaluated by adsorption kinetic, adsorption isotherm and adsorption selectivity. The results indicated that this material had not only binding properties but also high selectivity to the template molecule, which had good application prospects in the selective enrichment and separation of NP for pretreatment and analysis of complex environmental samples.

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A New Mechanistic Diagram for Molecularly Imprinted Polymers

MRS Proceedings ◽

10.1557/proc-787-g2.6 ◽

2003 ◽

Vol 787 ◽

Cited By ~ 1

Author(s):

David A. Spivak

Keyword(s):

Functional Groups ◽

Molecularly Imprinted Polymers ◽

Binding Sites ◽

Complex Structure ◽

Shape Selectivity ◽

Solution Phase ◽

Template Molecule ◽

Polymer Complex ◽

Molecularly Imprinted ◽

Imprinted Polymers

ABSTRACTA new mechanistic diagram describing the non-covalent molecular imprinting process is put forth in the text. A significant consequence of the new mechanistic picture is that the pre-polymer complex structure does not necessarily reflect the structure of the final binding sites in the polymer. Two independent studies are presented in combined form that support the suggested changes to the mechanistic diagram. In the first study, the maximum number of functional groups surrounding the template molecule in solution are shown to be less than the average number of functional groups in the binding sites of the polymers. In the second study, shape selectivity is shown to be an important contributor to molecular recognition by the imprinted polymers; which is significant because contributions of shape cannot be predicted by the solution phase pre-polymer complex.

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A Review on Molecularly Imprinted Polymers Preparation by Computational Simulation-Aided Methods

Polymers ◽

10.3390/polym13162657 ◽

2021 ◽

Vol 13 (16) ◽

pp. 2657

Author(s):

Zhimin Liu ◽

Zhigang Xu ◽

Dan Wang ◽

Yuming Yang ◽

Yunli Duan ◽

...

Keyword(s):

Molecular Simulation ◽

Molecularly Imprinted Polymers ◽

Computational Simulation ◽

Optimization Methods ◽

Intermolecular Forces ◽

Template Molecule ◽

Molecularly Imprinted ◽

Functional Monomers ◽

Imprinted Polymers ◽

Molecular Imprinting Technology

Molecularly imprinted polymers (MIPs) are obtained by initiating the polymerization of functional monomers surrounding a template molecule in the presence of crosslinkers and porogens. The best adsorption performance can be achieved by optimizing the polymerization conditions, but this process is time consuming and labor-intensive. Theoretical calculation based on calculation simulations and intermolecular forces is an effective method to solve this problem because it is convenient, versatile, environmentally friendly, and inexpensive. In this article, computational simulation modeling methods are introduced, and the theoretical optimization methods of various molecular simulation calculation software for preparing molecularly imprinted polymers are proposed. The progress in research on and application of molecularly imprinted polymers prepared by computational simulations and computational software in the past two decades are reviewed. Computer molecular simulation methods, including molecular mechanics, molecular dynamics and quantum mechanics, are universally applicable for the MIP-based materials. Furthermore, the new role of computational simulation in the future development of molecular imprinting technology is explored.

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Molecular Recognition: Perspective and a New Approach

Sensors ◽

10.3390/s21082757 ◽

2021 ◽

Vol 21 (8) ◽

pp. 2757

Author(s):

W. Rudolf Seitz ◽

Casey J. Grenier ◽

John R. Csoros ◽

Rongfang Yang ◽

Tianyu Ren

Keyword(s):

Molecular Recognition ◽

Molecularly Imprinted Polymers ◽

Binding Sites ◽

Binding Kinetics ◽

Molecularly Imprinted ◽

New Approach ◽

Imprinted Polymers ◽

High Affinity ◽

Water Blocking ◽

High Level

This perspective presents an overview of approaches to the preparation of molecular recognition agents for chemical sensing. These approaches include chemical synthesis, using catalysts from biological systems, partitioning, aptamers, antibodies and molecularly imprinted polymers. The latter three approaches are general in that they can be applied with a large number of analytes, both proteins and smaller molecules like drugs and hormones. Aptamers and antibodies bind analytes rapidly while molecularly imprinted polymers bind much more slowly. Most molecularly imprinted polymers, formed by polymerizing in the presence of a template, contain a high level of covalent crosslinker that causes the polymer to form a separate phase. This results in a material that is rigid with low affinity for analyte and slow binding kinetics. Our approach to templating is to use predominantly or exclusively noncovalent crosslinks. This results in soluble templated polymers that bind analyte rapidly with high affinity. The biggest challenge of this approach is that the chains are tangled when the templated polymer is dissolved in water, blocking access to binding sites.

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Application of Response Surface Methodology to Synthesize Appropriate Molecularly Imprinted Polymer for Diazinon

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.605.67 ◽

2014 ◽

Vol 605 ◽

pp. 67-70 ◽

Cited By ~ 1

Author(s):

Mohsen Rahiminezhad ◽

Seyed Jamaleddin Shahtaheri ◽

Mohammad Reza Ganjali ◽

Abbas Rahimi Rahimi Forushani

Keyword(s):

Molecular Imprinting ◽

Molecularly Imprinted Polymer ◽

Binding Sites ◽

Capillary Electrochromatography ◽

Template Molecule ◽

Imprinted Polymer ◽

Molecularly Imprinted ◽

Functional Monomers ◽

Molecular Imprinting Technology ◽

Cross Linker

Molecular imprinting technology has become an interesting research area to the preparation of specific sorbent material for environmental and occupational sample preparation techniques (1). In the molecular imprinting technology, specific binding sites have been formed in polymeric matrix, which often have an affinity and selectivity similar to antibody-antigen systems (2). In molecular imprinted technology, functional monomers are arranged in a complementary configuration around a template molecule, then, cross-linker and solvent are also added and the mixture is treated to give a porous material containing nono-sized binding sites. After extraction of the template molecule by washing, vacant imprinted sites will be left in polymer, which are available for rebinding of the template or its structural analogue (3). The stability, convention of preparation and low cost of these materials make them particularly attractive (4). These synthetic materials have been used for capillary electrochromatography (5), chromatography columns (6), sensors (7), and catalyze system (8). Depending on the molecular imprinting approach, different experimental variables such as the type and amounts of functional monomers, porogenic solvent, initiator, monomer to cross-linker ratio, temperature, and etc may alter the properties of the final polymeric materials. In this work, chemometric approach based on Central Composite Design (CCD) was used to design the experiments as well as to find the optimum conditions for preparing appropriate diazinon molecularly imprinted polymer.

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