Significant Enhancement of Triterpenoids Production by Phomopsis Elicitors in Cell Suspension Cultures of Betula Platyphylla Suk.

2011 ◽  
Vol 335-336 ◽  
pp. 105-110 ◽  
Author(s):  
Qiao Li Zhai ◽  
Ya Guang Zhan ◽  
Dao Qi Xu ◽  
Xiao Dong Wang ◽  
Gui Zhi Fan
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Endophytic Fungi ◽  
Molecular Level ◽  
Mrna Level ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Betula Platyphylla ◽  
Fungal Elicitors ◽  
Betula Platyphylla Suk

58 fungal elicitors prepared from the endophytic fungi isolated from inner bark of B. platyphylla Suk. were examined to determine their effects on the growth and triterpenoid production in suspension cultures of Betula platyphylla Suk. cells. The results showed that different fungal elicitors could cause diversely stimulating effects. Among the fungal elicitors tested, BE58 stimulated the highest triterpenoids yield with 15.90 mg•L-1 and 183.72% higher than the control. The experiment also affirmed from mRNA level that triterpenoid was indeed accumulated in suspension culture of birch cells treated by BE58 fungal elicitor. Meantime BE58 was identified as Phomopsis from the morphological and molecular level.

Proteomic analysis of changes in the extracellular matrix of Arabidopsis cell suspension cultures induced by fungal elicitors

PROTEOMICS ◽  
2003 ◽  
Vol 3 (6) ◽  
pp. 1047-1059 ◽  
Author(s):  
Bongani K. Ndimba ◽  
Stephen Chivasa ◽  
John M. Hamilton ◽  
William J. Simon ◽  
Antoni R. Slabas
Keyword(s):  
Extracellular Matrix ◽  
Cell Suspension ◽  
Proteomic Analysis ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Fungal Elicitors

scholarly journals Development of a Cell Suspension Culture System for Promoting Alkaloid and Vinca Alkaloid Biosynthesis Using Endophytic Fungi Isolated from Local Catharanthus roseus

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 672
Author(s):  
Tran My Linh ◽  
Nguyen Chi Mai ◽  
Pham Thi Hoe ◽  
Ninh Thi Ngoc ◽  
Phan Thi Hong Thao ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Endophytic Fungi ◽  
Catharanthus Roseus ◽  
Cell Suspension Culture ◽  
Indole Alkaloids ◽  
Vinca Alkaloid ◽  
Indole Alkaloid ◽  
Cell Suspension Cultures ◽  
Cell Extracts

Cell and tissue cultures of Catharanthus roseus have been studied extensively as an alternative strategy to improve the production of valuable secondary metabolites. The purpose of this study was to produce C. roseus callus and suspension cell biomass of good quality and quantity to improve the total alkaloids and bis-indole alkaloids. The young stem derived-callus of C. roseus variety Quang Ninh (QN) was grown on MS medium supplemented with 1.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) plus 1.5 mg/L kinetin, and the growth rate increased by 67-fold after 20 days. The optimal conditions for maintaining the cell suspension culture were 150 mg/50 mL cell inoculum, a medium pH of 5.5 and a culture temperature of 25 °C. The low alkaloid content in the culture was compensated for by using endophytic fungi isolated from local C. roseus. Cell extracts of endophytic fungi—identified as Fusarium solani RN1 and Chaetomium funicola RN3—were found to significantly promote alkaloid accumulation. This elicitation also stimulated the accumulation of a tested bis-indole alkaloid, vinblastine. The findings are important for investigating the effects of fungal elicitors on the biosynthesis of vinblastine and vincristine, as well as other terpenoid indole alkaloids (TIAs), in C. roseus QN cell suspension cultures.

scholarly journals Plant Regeneration from Cell Suspension Cultures of Betula platyphylla var. japonica.

1996 ◽  
Vol 13 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Yohichi WAKITA ◽  
Hamako SASAMOTO ◽  
Shinso YOKOTA ◽  
Nobuo YOSHIZAWA
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Betula Platyphylla

scholarly journals A Study On The Effect of Different Elicitors On Capsaicin Accumulation in Cell Suspension Cultures of Capsicum Assamicum (Bhut Jolokia)

2021 ◽  
Author(s):  
Swet Nisha ◽  
Ajitabh Bora ◽  
HK Gogoi ◽  
SK Dwivedi ◽  
PJ Handique
Keyword(s):  
Salicylic Acid ◽  
Suspension Culture ◽  
Methyl Jasmonate ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Sinapic Acid ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Exponential Phase ◽  
Bhut Jolokia

Abstract Elicitation of cell suspension cultures of Capsicum assamicum (Bhut Jolokia) for enhancement of capsaicin content was tried using different elicitors such as cellulase, vanillin, methyl jasmonate, salicylic acid and sinapic acid in different concentrations for 24, 48 and 72 hours. Cell suspension culture was established in B5 media supplemented with 3.5 mM 2,4-D (2,4-diphenoxyacetic acid) and 1.1 mM Kin and elicitors were introduced at the end of exponential phase. All the elicitors, except methyl jasmonate, led to significant increase in production of capsaicin. Sinapic acid, when added in 22 µM concentration and incubated for 24 hours, led to highest capsaicin accumulation of 0.5% (5068 µg/g) which was highest among all the treatments.

scholarly journals Elicitation of gymnemic acid production in cell suspension cultures of Gymnema sylvestre R.Br. through endophytic fungi

3 Biotech ◽  
2016 ◽  
Vol 6 (2) ◽  
Author(s):  
Vasudeva Reddy Netala ◽  
Venkata Subbaiah Kotakadi ◽  
Susmila Aparna Gaddam ◽  
Sukhendu Bikash Ghosh ◽  
Vijaya Tartte
Keyword(s):  
Cell Suspension ◽  
Endophytic Fungi ◽  
Acid Production ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Gymnema Sylvestre ◽  
Gymnemic Acid

scholarly journals Cell suspension culture of Amsonia tabernaemontana Walter: growth, organogenesis and alkaloid production

2014 ◽  
Vol 50 (4) ◽  
pp. 615-624 ◽  
Author(s):  
Mirosława Furmanowa ◽  
Lucyna Rapczewska
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Cell Suspension Cultures ◽  
Cell Number ◽  
Growth Increment ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Alkaloid Production ◽  
Cellular Aggregates

The paper discusses the growth of cell suspension cultures of <em>Amsonia tabernaemontana</em> Walter established from callus of hypocotyl origin. The cell number and growth increment were determined. Cellular aggregates developed well in the Wood and Braun (WB) medium with 1 mg/l NAA and 0.5 mg/l kinetin (growth increment 712.4). When the aggregates were cultured on WB media without NAA and kinetin or with 0.02 mg/l kinetin and 3 mg/l IAA, Toots developed an the aggregates. Examiination of the roots and cell suspensions indicates that the Toots are richer in alkaloids than the callus and cell suspensions.

Biotransformation of (–)-Caryophyllene Oxide by Cell Suspension Culture of Catharanthus roseus

2006 ◽  
Vol 61 (2) ◽  
pp. 197-200 ◽  
Author(s):  
Muhammad Iqbal Choudhary ◽  
Zafar A. Siddiqui ◽  
Saifullah Khan ◽  
Syed G. Musharraf ◽  
◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Cell Suspension Culture ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Spectroscopic Techniques ◽  
Caryophyllene Oxide ◽  
New Compounds

AbstractCatharanthus roseus cell suspension cultures were employed for the biotransformation of (-)-caryophyllene oxide (1), and four metabolites, 15-hydroxycaryophyllene oxide (2), 4β ,5α- dihydroxycaryophyll-8(13)-ene (3), 2β -hydroxycaryophyllene oxide (4), and 2-hydroxy-4,5- epoxycaryophyllan-13-ol (5) were obtained. Metabolites 4 and 5 were found to be new compounds, and their structures were deduced by different spectroscopic techniques.

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