Analysis of Synthetic Antioxidant in Food by Capillary Electrophoresis

2011 ◽  
Vol 361-363 ◽  
pp. 1855-1858 ◽  
Author(s):  
Qian Xiang ◽  
Ying Gao
Keyword(s):  
Capillary Electrophoresis ◽  
Amperometric Detection ◽  
Peak Height ◽  
Butylated Hydroxyanisole ◽  
Applied Potential ◽  
Synthetic Antioxidant ◽  
Separation Potential ◽  
Relative Standard ◽  
Capillary Electrophoretic

A capillary electrophoretic assay for determining synthetic antioxidant butylated hydroxyanisole in food has been developed. The extraction with 70% (v/v) methanol quantitatively extracted synthetic antioxidant. The separation was carried out by CZE using phosphate at a separation potential of 18 kV. Amperometric detection was achieved with an applied potential of 0.60 V. A linear relationship between the peak height and the concentration of the analyte was found in the range 1.8-180 µg/mL for BHA, with correlation coefficient of 0.994. The relative standard deviations of migration time and peak height were 0.19 and 5.3 %, respectively. The method developed was successfully applied for the determination of synthetic antioxidant butylated hydroxyanisole in food. Recovery of butylated hydroxyanisole was 93%.

Determination of Effective Ingredients in Compound Sulfamethoxazole Tablets by Capillary Electrophoresis with Amperometric Detection

2008 ◽  
Vol 36 (3) ◽  
pp. 292-296 ◽  
Author(s):  
Chu Qing-Cui ◽  
Tian Xiu-Hui ◽  
Jiang Lian-Mei ◽  
Zhou Wen-Jun ◽  
Ye Jian-Nong
Keyword(s):  
Capillary Electrophoresis ◽  
Amperometric Detection

Reliable Method for the Determination of Food Additive by Capillary Electrophoresis Using a Microvolume of Foodstuffs

2011 ◽  
Vol 361-363 ◽  
pp. 1486-1489
Author(s):  
Qian Xiang ◽  
Ying Gao
Keyword(s):  
Capillary Electrophoresis ◽  
Reliable Method ◽  
Lower Cost ◽  
Limit Of Detection ◽  
Food Additive ◽  
Fast Method ◽  
Optimum Conditions ◽  
Applied Potential ◽  
Minimal Sample

A fast method for the separation and determination of the food additive propyl gallate has been established by using capillary electrophoresis. The effects of several factors such as the applied potential and detection running buffer were investigated in order to obtain the optimum conditions, and the assay results were satisfactory. The limit of detection for the analyte was 10-6 mol/L. This approach has remarkable advantages with respect to other methodologies involving separations and electrochemical detection including minimal sample consumption, higher analysis speed and lower cost. In order to demonstrate the capabilitiy of the method, the determination of additive in a commercial food sample is also presented.

Liquid Chromatographic Determination of Quinine, Hydroquinine, Saccharin, and Sodium Benzoate in Quinine Beverages

1985 ◽  
Vol 68 (4) ◽  
pp. 782-784
Author(s):  
Leonard P Valenti
Keyword(s):  
Sodium Benzoate ◽  
Direct Injection ◽  
Chromatographic Determination ◽  
Peak Height ◽  
Relative Standard ◽  
Sodium Saccharin ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
The Relationship

Abstract A liquid chromatographic (LC) method is described for the determination of quinine, hydroquinine, sodium saccharin, and sodium benzoate in soft drinks. The method involves simple sample preparation, direct injection onto an octadecylsilane column, and elution with a methanol-acetonitrile-water-acetic acid (20 + 10 + 70 + 1) mobile phase. Eluted constituents are measured spectrophotometrically at 254 nm. The relationship between peak height and concentration was linear between 20 and 120 μg/mL for quinine. A relative standard deviation of 0.82% was obtained for commercial samples spiked with quinine, and the average recovery was 100.3%. The proposed procedure is accurate and rapid and can also detect hydroquinine (a natural contaminant of quinine), sodium saccharin, and sodium benzoate. Linear responses ranged from 0.45 to 20 (xg/mL for hydroquinine, from 54.8 to 219 μg/mL for sodium saccharin, and from 10.1 to 145.1 (ig/mL for sodium benzoate. The reproducibility of the LC method was evaluated with standard solutions of hydroquinine, sodium saccharin, and sodium benzoate, which produced relative standard deviations of 0.42, 0.46, and 1.13%, respectively. The average recoveries for sodium saccharin and sodium benzoate from spiked samples were 99.4 and 100.2%, respectively.

scholarly journals Fast and direct determination of butylated hydroxyanisole in biodiesel by batch injection analysis with amperometric detection

Talanta ◽  
2011 ◽  
Vol 85 (3) ◽  
pp. 1274-1278 ◽  
Author(s):  
Thiago Faria Tormin ◽  
Denise Tofanello Gimenes ◽  
Eduardo Mathias Richter ◽  
Rodrigo Alejandro Abarza Munoz
Keyword(s):  
Direct Determination ◽  
Amperometric Detection ◽  
Butylated Hydroxyanisole

scholarly journals Determination of cyanide in bamboo shoots by microdiffusion combined with ion chromatography–pulsed amperometric detection

2018 ◽  
Vol 5 (4) ◽  
pp. 172128 ◽  
Author(s):  
Ming Ding ◽  
Kailiang Wang
Keyword(s):  
Ion Chromatography ◽  
Limit Of Detection ◽  
Correlation Coefficients ◽  
Amperometric Detection ◽  
High Sensitivity ◽  
Practical Method ◽  
Pulsed Amperometric Detection ◽  
Bamboo Shoots ◽  
Relative Standard

A practical method for the determination of cyanide in bamboo shoots has been developed using microdiffusion preparation integrated with ion chromatography–pulsed amperometric detection (IC-PAD). Cyanide was released from bamboo shoots after Conway cell microdiffusion, and then analysed by IC-PAD. In comparison with the previously reported methods, derivatization and ion-pairing agent addition were not required in this proposed microdiffusion combined with IC-PAD method. The microdiffusion parameters were optimized including hydrolysis systems, temperature, time, and so on. Under the optimum conditions, the linear range of the calibration curve for cyanide was 0.2–200.0 µg kg −1 with satisfactory correlation coefficients of 0.9996 and the limit of detection was 0.2 µg kg −1 ( S/N  = 3). The spiked recovery range was from 92.8 to 98.6%. The intra-day and inter-day relative standard deviations of cyanide were 2.7–14.9% and 3.0–18.3%, respectively. This method was proved to be convenient in operation with high sensitivity, precision and accuracy, and was successfully applied in the determination of cyanide in bamboo shoot samples.

Capillary electrophoresis of wheat gliadin proteins and its potential for wheat varietal identification using pattern matching software

2001 ◽  
Vol 52 (8) ◽  
pp. 839 ◽  
Author(s):  
S. Siriamornpun ◽  
M. Wootton ◽  
J. M. Cox ◽  
F. Bekes ◽  
C. W. Wrigley
Keyword(s):  
Capillary Electrophoresis ◽  
Pattern Matching ◽  
Processing Parameters ◽  
Peak Height ◽  
Fused Silica ◽  
Constant Current ◽  
Good Resolution ◽  
Relative Mobility ◽  
Window Width ◽  
Relative Standard

Gliadins from 11 wheat flours were extracted with 30% ethanol and fractionated by capillary electrophoresis on a 20-µm i.d. untreated fused silica capillary using 0.1 M phosphate buffer (pH 2.5) containing polymer modifier. Capillary electrophoresis conducted at a constant current provided very good resolution and reproducibility (relative standard deviation <0.5) in mp;lt;15 min. Pattern matching of the profiles was performed with the PatMatch program to provide quantitative comparisons, using the relative mobility and intensity data for each gliadin protein. Data processing parameters, including the integration of the electrophoregram, were optimised for separation of gliadins extracted from either whole-grain or flour samples. The reproducibility and repeatability were compared using peak height and/or area percentages. The optimal window width for identifying matching gliadin peaks was 0.80–1.20% relative mobility units. Using these conditions, it was concluded that unknown varieties could be identified with a confidence level of 90–95%.

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