FTIR/ATR Spectroscopy with Stability for Determination of HGB in Human Whole Blood

2012 ◽  
Vol 468-471 ◽  
pp. 1663-1666
Author(s):  
Tao Pan ◽  
Wen Jie Huang ◽  
Hao Yin
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Whole Blood ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Human Whole Blood ◽  
Reliable Model ◽  
Smoothing Parameters ◽  
Atr Spectroscopy

Fourier transform infrared spectroscopy (FTIR) and attenuated total reflection (ATR) combined with partial least-squares (PLS) and Savitzky-Golay (SG) smoothing were successfully applied for rapid no-reagent determination of hemoglobin (HGB) in human whole blood. The optimal SG smoothing parameters OD, DP, NSP and PLS factor were 1, 2, 49 and 14, respectively. The modeling effect M-SEPAve, M-RP,Ave, M-SEPStd, and M-RP,Std were 6.73g/L, 0.850, 0.306g/L, and 0.015, respectively. The validation effect V-SEP and V-RP were 6.83g/L and 0.864, respectively. The method provided a reliable model with stability for clinic application of FTIR/ATR.

Multivariate determination of glucose in whole blood by attenuated total reflection infrared spectroscopy

1989 ◽  
Vol 61 (18) ◽  
pp. 2009-2015 ◽  
Author(s):  
H. M. Heise ◽  
Ralf. Marbach ◽  
Guenter. Janatsch ◽  
J. D. Kruse-Jarres
Keyword(s):  
Infrared Spectroscopy ◽  
Whole Blood ◽  
Total Reflection ◽  
Attenuated Total Reflection

Effects of Hydrogen Peroxide on Human Dentin Structure

2007 ◽  
Vol 86 (11) ◽  
pp. 1040-1045 ◽  
Author(s):  
T. Jiang ◽  
X. Ma ◽  
Y. Wang ◽  
Z. Zhu ◽  
H. Tong ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Human Dentin ◽  
Mineral Components ◽  
Atr Spectroscopy ◽  
Demineralized Dentin ◽  
Chemical Evidence

It has been hypothesized that hydrogen peroxide (H2O2) bleaching may cause destruction of dentin by a mechanism of protein oxidation. However, to our knowledge, there has been no direct chemical evidence to validate this viewpoint. To investigate the effects of H2O2 on the structure of human dentin, we used Fourier transform infrared spectroscopy (FTIR) and attenuated total reflection (ATR) spectroscopy. Human intact dentin specimens were treated either with 30% H2O2 or Hanks’ balanced salt solution (HBSS). Significant differences were observed in ATR spectra parameters. Additionally, demineralized dentin specimens were also tested. They were completely dissolved in the H2O2, but remained intact in the 0.1 N HCl and HBSS. The results suggested that H2O2 attacked both the organic and mineral components of dentin. Destruction of the organic components was mainly because of the oxidizing ability of H2O2, while changes in the mineral components were probably due to its acidity.

Determination of Triglycerides on Cotton Fabrics Using Fourier Transform Attenuated Total Reflection Infrared Spectroscopy

1985 ◽  
Vol 39 (6) ◽  
pp. 1047-1050 ◽  
Author(s):  
R. S. Shreedhara Murthy ◽  
D. E. Leyden ◽  
J. Keith Grime
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Ir Spectroscopy ◽  
Cotton Fabric ◽  
Cotton Fabrics ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Triglycerides deposited on cotton fabric in the form of artificial body soil (ABS) were quantified with the use of ATR/FT-IR spectroscopy after ABS had been transferred onto the ATR crystal under pressure. This approach improved the signal-to-background ratio by avoiding interference from cellulose absorption, and improved precision as contact of the cloth sample with the ATR crystal was not necessary. ABS concentrations comparable to natural body soil levels were determined. This procedure was shown to be useful for following the depletion of “surface” ABS after different washing procedures.

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