Multivariate determination of glucose in whole blood by attenuated total reflection infrared spectroscopy

1989 ◽  
Vol 61 (18) ◽  
pp. 2009-2015 ◽  
Author(s):  
H. M. Heise ◽  
Ralf. Marbach ◽  
Guenter. Janatsch ◽  
J. D. Kruse-Jarres
Keyword(s):  
Infrared Spectroscopy ◽  
Whole Blood ◽  
Total Reflection ◽  
Attenuated Total Reflection

FTIR/ATR Spectroscopy with Stability for Determination of HGB in Human Whole Blood

2012 ◽  
Vol 468-471 ◽  
pp. 1663-1666
Author(s):  
Tao Pan ◽  
Wen Jie Huang ◽  
Hao Yin
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Whole Blood ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Human Whole Blood ◽  
Reliable Model ◽  
Smoothing Parameters ◽  
Atr Spectroscopy

Fourier transform infrared spectroscopy (FTIR) and attenuated total reflection (ATR) combined with partial least-squares (PLS) and Savitzky-Golay (SG) smoothing were successfully applied for rapid no-reagent determination of hemoglobin (HGB) in human whole blood. The optimal SG smoothing parameters OD, DP, NSP and PLS factor were 1, 2, 49 and 14, respectively. The modeling effect M-SEPAve, M-RP,Ave, M-SEPStd, and M-RP,Std were 6.73g/L, 0.850, 0.306g/L, and 0.015, respectively. The validation effect V-SEP and V-RP were 6.83g/L and 0.864, respectively. The method provided a reliable model with stability for clinic application of FTIR/ATR.

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