Preparation and Characterization of Immobilized α-Amylase

2012 ◽  
Vol 602-604 ◽  
pp. 1186-1189
Author(s):  
Zhi Jie Geng ◽  
Song Bai Lin ◽  
Gao Shuang Huang ◽  
Ai Ru Ke
Keyword(s):  
Aqueous Solution ◽  
Enzymatic Activity ◽  
Immobilized Enzyme ◽  
Kinetic Studies ◽  
Maximum Activity ◽  
Solution Polymerization ◽  
Adsorption Method ◽  
Aqueous Solution Polymerization

P(DMAA-co-AM)hydrogel; adsorption method; a-amylase; Immobilized enzyme. Abstract. P(DMAA-co-AM)hydrogel was prepared by aqueous solution polymerization with N,N-dimethylacrylamide(DMAA) and acrylamide(AM) as monomers.,taking the P(DMAA-co-AM) hydrogel as carrier, a-amylase was immobilized by adsorption method.The investigation of effection of temperature and pH on enzymatic activity exhibites that the optimum temperatures are 50°C for both free and immobilized enzyme,the maximum activity is observed at pH 6.0 for both free and immobized enzyme. The activity of immobilized a-amylase remaines higher 40% at sixth run. Kinetic studies demonstrate that: Km values are 5.15749 and 1.44479 mg/mL for free and immobilized enzyme, Vmax values are 2.56082 and 0.58272 mg/mL min-1 for free and immobilized enzyme.

scholarly journals Study about Doping Ion La3+ onto Surface of Pyrolusite Ore for Removing Simultaneously Both Fluoride and Phosphate from Wastewater

2017 ◽  
Vol 2017 ◽  
pp. 1-11
Author(s):  
Nguyen Thi Hue ◽  
Nguyen Hoang Tung
Keyword(s):  
Aqueous Solution ◽  
Kinetic Studies ◽  
Phosphate Adsorption ◽  
Fluoride Removal ◽  
Fluoride Adsorption ◽  
Pseudo Second Order ◽  
Xrd Techniques ◽  
Adsorbent Characterization ◽  
Coexisting Ions

Lanthanum has been doped onto the surface of the natural Pyrolusite for simultaneous removal of phosphate and fluoride in aqueous solution. The adsorbent characterization of the materials was observed by the SEM, BET, and XRD techniques. The dynamics and isotherms models of fluoride and phosphate adsorption, with respect to pH, pHPZC, adsorbent dose, and effect of coexisting ions, were studied. The results showed that lanthanum doped Pyrolusite ore (LDPO) relatively highly adsorbed amount of phosphate and fluoride from aqueous solution. Phosphate and fluoride removal efficiencies of LDPO are approximately 97% and 95%, respectively. Pseudo-first order for kinetic studies of phosphate and fluoride removal of the LDPO was observed with high correlations for fluoride but weak correlations for phosphate. However, pseudo-second order for kinetic studies was high correlation for both phosphate and fluoride. The phosphate and fluoride adsorption capacities of the LDPO significantly decreased with the existence of coions (sulfate, chloride, and nitrate) in the aqueous solution.

Properties and Synthesis of Kaolin / Potassium Polyacrylate / Acrylamide Composite Superabsorbent Resin

2011 ◽  
Vol 287-290 ◽  
pp. 1331-1334
Author(s):  
Hao Ren ◽  
Jie Li ◽  
Yu Fen Zhang ◽  
Tai Zhong Huang
Keyword(s):  
Aqueous Solution ◽  
Saline Water ◽  
Monomer Concentration ◽  
Water Absorbency ◽  
Solution Polymerization ◽  
Synthetic Resin ◽  
Polymer Molecules ◽  
Aqueous Solution Polymerization ◽  
Crosslinker Concentration ◽  
Superabsorbent Resin

The aqueous solution polymerization methods were used for the synthesis of superabsorbent resin. The effects of different experimental factors on synthetic resin water absorbency were investigated in this paper. The variables examined in this study included monomer concentration, neutralization degree, initiator and crosslinker concentration, reaction temperature and time, etc. The maximum absorbencies of distilled and 0.9% saline water are 919 times and 92 times respectively under optimum condition. Analysis from FTIR and SEM shown the polymer molecules can be firmly fixed with kaolin, the bonding of kaolin and polymer is good in CSR.

Adsorption characterization of Pb(ii) ions onto iodate doped chitosan composite: equilibrium and kinetic studies

RSC Advances ◽  
2015 ◽  
Vol 5 (67) ◽  
pp. 54188-54201 ◽  
Author(s):  
Asha H. Gedam ◽  
Rajendra S. Dongre
Keyword(s):  
Aqueous Solution ◽  
Kinetic Studies ◽  
Chitosan Composite

Iodate doped chitosan (I-CS) composite was synthesized, characterized and used as an adsorbent for adsorption of Pb(ii) ions from aqueous solution.

Semibatch Aqueous-Solution Polymerization of Acrylic Acid: Simultaneous Control of Molar Masses and Reaction Temperature

2011 ◽  
Vol 5 (5-6) ◽  
pp. 223-231 ◽  
Author(s):  
Roque J. Minari ◽  
Gerardo Caceres ◽  
Paola Mandelli ◽  
Mariana M. Yossen ◽  
Manuel Gonzalez-Sierra ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Acrylic Acid ◽  
Reaction Temperature ◽  
Solution Polymerization ◽  
Simultaneous Control ◽  
Aqueous Solution Polymerization

Preparation of TiO2/Polyacrylic Acid and Application in Photocatalytic Degradation of Methyl Orange

2009 ◽  
Vol 17 (4) ◽  
pp. 195-203
Author(s):  
Shanxia Luo ◽  
Hui Zhu ◽  
Fumin Wang ◽  
Feng Xin ◽  
Xiaohong Yin
Keyword(s):  
Aqueous Solution ◽  
Methyl Orange ◽  
Organic Compound ◽  
Polyacrylic Acid ◽  
Fine Particles ◽  
Absorption Characteristic ◽  
Solution Polymerization ◽  
Good Activity ◽  
Aqueous Solution Polymerization ◽  
Degradation Of Methyl Orange

TiO2 (P25) fine particles were loaded in a polyacrylic acid (PAA), which possessed a unique absorption characteristic and was synthesised by aqueous solution polymerization. The TiO2/PAA composite was taken as a photocatalyst to degrade methyl orange (MO), the degradability resistance and reproducibility of the photocatalyst were investigated experimentally. The results showed that the TiO2/PAA photocatalyst had a good activity and stability in degradability of organic compound.

A Novel Cement Fluid Loss Additive P1402

2014 ◽  
Vol 941-944 ◽  
pp. 1203-1207 ◽  
Author(s):  
Qiang Xiao ◽  
Wen Fa Xiao ◽  
Xin Xin Liu
Keyword(s):  
Thermal Stability ◽  
Aqueous Solution ◽  
High Temperature ◽  
Reaction Time ◽  
Performance Testing ◽  
Fluid Loss ◽  
Solution Polymerization ◽  
Excellent Thermal Stability ◽  
Aqueous Solution Polymerization ◽  
Fluid Loss Additive

A novel cement fluid loss additive P1402, which synthesized using the monomers of 2-acrylamido-2-methyl-propane sulphonic acid (AMPS), acrylic acid (AA), N,N dimethyl acrylamide (DMAM) and N-Vinyl-2-pyrrolidone (NVP) by the method of aqueous solution polymerization. The ratio of monomers AMPS:AA:DMAM:NVP at 2:1.5:1:1, reaction PH at 10.8, reaction temperature at 65°Cand reaction time about 5 hours. The IR spectrum of P1402 show that the polymer with the structure of all the monomers .The fluid loss performance testing show that the forpolymers P1402 has an excellent thermal stability. The fluid loss additive P1402 has an excellent tolerance to salt and high temperature.

scholarly journals Functional and biochemical characterization of a recombinant Arabidopsis thaliana 3-deoxy-D-manno-octulosonate 8-phosphate synthase

2004 ◽  
Vol 381 (1) ◽  
pp. 185-193 ◽  
Author(s):  
Jing WU ◽  
Mayur A. PATEL ◽  
Appavu K. SUNDARAM ◽  
Ronald W. WOODARD
Keyword(s):  
Arabidopsis Thaliana ◽  
Molecular Mass ◽  
Biochemical Characterization ◽  
Dipicolinic Acid ◽  
Kinetic Studies ◽  
Maximum Activity ◽  
Reading Frame ◽  
Sequential Mechanism ◽  
Phosphate Synthase

An open reading frame, encoding for KDOPS (3-deoxy-D-manno-octulosonate 8-phosphate synthase), from Arabidopsis thaliana was cloned into a T7-driven expression vector. The protein was overexpressed in Escherichia coli and purified to homogeneity. Recombinant A. thaliana KDOPS, in solution, displays an apparent molecular mass of 76 kDa and a subunit molecular mass of 31.519 kDa. Unlike previously studied bacterial KDOPSs, which are tetrameric, A. thaliana KDOPS appears to be a dimer in solution. The optimum temperature of the enzyme is 65 °C and the optimum pH is 7.5, with a broad peak between pH 6.5 and 9.5 showing 90% of maximum activity. The enzyme cannot be inactivated by EDTA or dipicolinic acid treatment, nor it can be activated by a series of bivalent metal ions, suggesting that it is a non-metallo-enzyme, as opposed to the initial prediction that it would be a metallo-enzyme. Kinetic studies showed that the enzyme follows a sequential mechanism with Km=3.6 μM for phosphoenolpyruvate and 3.8 μM for D-arabinose 5-phosphate and kcat=5.9 s−1 at 37 °C. On the basis of the characterization of A. thaliana KDOPS and phylogenetic analysis, plant KDOPSs may represent a new, distinct class of KDOPSs.

Study on the Preparation and Properties of PAA/BC/Na-MMT Composites

2011 ◽  
Vol 236-238 ◽  
pp. 1766-1769
Author(s):  
Hui Ling Shen ◽  
Li Juan Long ◽  
Shi Ru Jia
Keyword(s):  
Thermal Stability ◽  
Aqueous Solution ◽  
Network Structure ◽  
Layered Structure ◽  
Polyacrylic Acid ◽  
Sem Analysis ◽  
Solution Polymerization ◽  
Aqueous Solution Polymerization ◽  
Polymerization Method ◽  
Thermal Stability Of

By means of aqueous solution polymerization method, the composites of polyacrylic acid (PAA)/Bacterial cellulose (BC)/Na-MMT and PAA/Na-MMT were prepared respectively. The effects of Na-MMT content on the properties of PAA/Na-MMT and BC content on the properties of PAA/BC/Na-MMT composites were investigated. The results show that with appropriate increasing Na-MMT, the composites has good salt-resistant ability and thermal stability, mean while, the water-absorbing capacity of PAA/Na-MMT composites is significantly increased. Comparing with that of the PAA/Na-MMT hydrogel, with addition of BC content, the water-absorbing capacity and thermal stability of PAA/BC/Na-MMT are enhanced in a certain degree. The SEM analysis indicate that there are more network structure in the composites hydrogel. XRD result shows that the layered structure of Na-MMT is stripped and the prepared composites achieves peel compound.

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