Preliminary Study for the Allelopathic Effect of Water Extracts from Solidago canadensis Leaves

2013 ◽  
Vol 699 ◽  
pp. 340-348 ◽  
Author(s):  
Shu Lin Li ◽  
Zhao Hui Li ◽  
Ya Fei Wang ◽  
Xiao Ruan ◽  
Cun De Pan ◽  
...  

The allelopathy of the exotic invasive plant, Solidago canadensis L. was studied. S. canadensis leaves were extracted with water and partitioned against ethyl acetate and n-butanol sequentially. The two organic fractions, original water extract and the residual water after the organic solvent extraction were tested for inhibitory activity on seed germination and seedling growth of wheat (Triticum aestivum), setaria (Setaria viridis) and its own species. Germination rate, vigor and index were recorded as germination parameters, and radicle and plumule growth and fresh weight of seedlings were recorded as seedling growth parameters. Results showed that the water extracts of S. Canadensis leaves had significant impacts on the seed germination and seedling growth of the three tested plants. n-Butanol fraction showed the strongest allelopathic effect, while the residual water after the organic solvent extraction had no significant phytotoxic effect compared with the control. n-Butanol fraction at 0.5 mg•ml-1 could significantly inhibited the seed germination of the same species, and that at 5 mg•ml-1 could significantly inhibited the seedling growth of the same species. n-Butanol fraction at 1.5 mg•ml-1 could significantly inhibited the seed germination and seedling growth of wheat. n-Butanol fraction at 5 mg•ml-1 could significantly inhibited the seed germination and seedling growth of setaria. The allelochemicals were mainly contained in n-butanol fraction of water extracts from S. canadensis leaves.

1972 ◽  
Vol 69 (2) ◽  
pp. 257-266 ◽  
Author(s):  
Bryant Benson ◽  
Mary Jane Matthews ◽  
Alvin E. Rodin

ABSTRACT Continuing investigation of pineal gland function indicates that the anti-gonadotrophic activity of this organ cannot be attributed solely to the postulated hormone melatonin, the concentration of which is negligible in the pineal body compared to quantities required to produce unequivocal physiological effects. A non-melatonin antigonadotrophic substance recently isolated from bovine pineal glands was further purified by organic solvent extraction, ultrafiltration and gel filtration. Studies of partial blockage of compensatory ovarian hypertrophy in unilaterally ovariectomized Charles River CD-1 mice indicated that this substance is significantly more potent than melatonin in this test system.


Holzforschung ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Xiaodi Wang ◽  
Yongchao Zhang ◽  
Luyao Wang ◽  
Xiaoju Wang ◽  
Qingxi Hou ◽  
...  

AbstractAn efficient separation technology for hydrolysates towards a full valorization of bamboo is still a tough challenge, especially regarding the lignin and lignin-carbohydrate complexes (LCCs). The present study aimed to develop a facile approach using organic solvent extraction for efficiently fractionating the main components of bamboo hydrolysates. The high-purity lignin with only a trace of carbohydrates was first obtained by precipitation of the bamboo hydrolysate. The water-soluble lignin (WSL) fraction was extracted in organic solvent through a three-stage organic solvent extraction process, and the hemicellulosic sugars with increased purity were also collected. Furthermore, a thorough characterization including various NMR techniques (31P, 13C, and 2D-HSQC), GPC, and GC-MS was conducted to the obtained lignin-rich-fractions. It was found that the WSL fraction contained abundant functional groups and tremendous amount of LCC structures. As compared to native LCC of bamboo, the WSL fraction exhibited more typical LCC linkages, i.e. phenyl glycoside linkage, which is the main type of chemical linkage between lignin and carbohydrate in both LCC samples. The results demonstrate that organic phase extraction is a highly efficient protocol for the fractionation of hydrolysate and the isolation of LCC-rich streams possessing great potential applications.


2009 ◽  
Vol 877 (18-19) ◽  
pp. 1751-1757 ◽  
Author(s):  
Zhang Ying ◽  
A Jiye ◽  
Guangji Wang ◽  
Huang Qing ◽  
Yan Bei ◽  
...  

Author(s):  
Weihong Sun ◽  
Guofeng Yang ◽  
Lili Cong ◽  
Juan Sun ◽  
Lichao Ma

Background: Plant allelopathy refers to the release of chemicals from plants or microorganisms into the environment, may have direct or indirect, beneficial or harmful effects on other plants or microorganisms. When plants grow in an unfavorable environment,more allelochemicals will be secreted and the expression of allelopathic effects will increase, giving plants a certain competitive advantage. Hairy vetch is one of the most promising allelopathic crops and the aqueous extract of hairy vetch has an inhibitory effect on the root length and seedling height of grass crops. The current study aimed to study the allelopathic effect of hairy vetch on alfalfa, and exploring an ecological method to remove the root system of alfalfa.Methods: In this experiment, the allelopathic effects of the seeds, stems and leaves, roots extracts and root exudates (0, 3, 6, 9 and 12 mg·mL-1) on the seed germination and seedling growth of alfalfa were evaluated. And the main allelopathic substances from the stem and leaf extract were isolated and identified using high performance liquid chromatography- mass spectrometry (HPLC-MS).Result: The results showed that all of the extracts can inhibit alfalfa seed germination and seedling growth and stem and leaf extract had the strongest inhibitory effect, especially for inhibiting the root growth. A main allelochemical substance, o-coumaric acid, was screened out and the root length of alfalfa was completely inhibited at 1.6 mg·mL-1 of o-coumaric acid. The findings of these experiments show hairy vetch has strong allelopathic effect on alfalfa and o-coumaric acid is a chemical growth inhibitor.


2002 ◽  
Vol 118 (2) ◽  
pp. 136-140
Author(s):  
Takahiro YOSHIDA ◽  
Toshimasa TAKANOHASHI ◽  
Kinya SAKANISHI ◽  
Ikuo SAITO ◽  
Masaharu FUJITA ◽  
...  

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