Allelopathic Potency and an Active Substance from Hairy Vetch

2020 ◽  
Author(s):  
Weihong Sun ◽  
Guofeng Yang ◽  
Lili Cong ◽  
Juan Sun ◽  
Lichao Ma
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Root Length ◽  
Leaf Extract ◽  
Growth Inhibitor ◽  
Inhibitory Effect ◽  
Allelopathic Effect ◽  
Coumaric Acid ◽  
Hairy Vetch ◽  
Allelopathic Effects

Background: Plant allelopathy refers to the release of chemicals from plants or microorganisms into the environment, may have direct or indirect, beneficial or harmful effects on other plants or microorganisms. When plants grow in an unfavorable environment,more allelochemicals will be secreted and the expression of allelopathic effects will increase, giving plants a certain competitive advantage. Hairy vetch is one of the most promising allelopathic crops and the aqueous extract of hairy vetch has an inhibitory effect on the root length and seedling height of grass crops. The current study aimed to study the allelopathic effect of hairy vetch on alfalfa, and exploring an ecological method to remove the root system of alfalfa.Methods: In this experiment, the allelopathic effects of the seeds, stems and leaves, roots extracts and root exudates (0, 3, 6, 9 and 12 mg·mL-1) on the seed germination and seedling growth of alfalfa were evaluated. And the main allelopathic substances from the stem and leaf extract were isolated and identified using high performance liquid chromatography- mass spectrometry (HPLC-MS).Result: The results showed that all of the extracts can inhibit alfalfa seed germination and seedling growth and stem and leaf extract had the strongest inhibitory effect, especially for inhibiting the root growth. A main allelochemical substance, o-coumaric acid, was screened out and the root length of alfalfa was completely inhibited at 1.6 mg·mL-1 of o-coumaric acid. The findings of these experiments show hairy vetch has strong allelopathic effect on alfalfa and o-coumaric acid is a chemical growth inhibitor.

scholarly journals Propagation and Possible Allelopathic Effects of Vernoniazeylanica on Selected Bioassay Species

CORD ◽  
2020 ◽  
Vol 36 ◽  
pp. 41-46
Author(s):  
S.H.S. Senarathne ◽  
S. S. Udumann
Keyword(s):  
Seed Germination ◽  
Plant Species ◽  
Seedling Growth ◽  
Contaminated Soil ◽  
Leaf Extract ◽  
Cocos Nucifera ◽  
Test Plant ◽  
Allelopathic Effect ◽  
Aqueous Leaf Extract ◽  
Allelopathic Effects

Vernonia zeylanica (L.) belongs to the family Asteraceae, is one of the major endemic weed species present in coconut (Cocos nucifera L.) plantations of the tropics, which propagates very easily.  There is a possibility that this plant could also possess allelopathic effects, but this has not been scientifically tested.  Thus, a study was carried out to determine the seed germination of V. zeylanica under soil moisture stress conditions, shoot propagation methods and possible allopathic effects of this species, on selected species in bioassay tests.  Germination of V. zeylanica seeds was not observed at higher osmotic potential (-0.9 MPa).  The highest sprouting percentage of this species were obtained with soft wood cuttings.  The aqueous leaf extract was highly phytotoxic, and it significantly reduced germination and seedling growth of all bioassay species tested. Full strength (33.3 g L-1) aqueous extracts of leaves significantly reduced the germination percentage, root and hypocotyl growth rates of all species tested.  The inhibitory effects were often dependent on concentration.  However, the degree of inhibition varied among the test plant species.  The seedling emergences of all four tested plants were severally inhibited when planted in V. zeylanica contaminated soil.  The results indicated that incorporated aqueous leaf extract of V. zeylanica and its rhizosphere contaminated soil can suppress seed germination, seedling growth and seedling emergence of certain plant species indicating a possible allelopathic effect.

scholarly journals Allelopathic Effect of Artemisia Dubia Extracts on Seed Germination and Seedling Growth of Some Weeds and Winter Crops

2015 ◽  
Vol 21 ◽  
pp. 23-30
Author(s):  
Baby Babita Das Mallik ◽  
BD Acharya ◽  
M Saquib ◽  
MK Chettri
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Leaf Extract ◽  
Cyperus Rotundus ◽  
Allelopathic Effect ◽  
Ageratum Conyzoides ◽  
Bidens Pilosa ◽  
Winter Crops ◽  
Allelopathic Effects ◽  
Artemisia Dubia

Allelopathic effects of aqueous extract of different plant parts (root, stem and leaf) of Artemisia dubia on seed germination and seedling growth of two winter crops (Triticum aestivum and Brassica campestries, and some associated weeds (Bidens pilosa, Ageratum conyzoides, Galinsoga parviflora and Cyperus rotundus) have been investigated in the present study. Extracts of root, stem and leaves of Artemisia dubia showed significant reduction in germination and seedling growth of test crops and weeds. Germination of crop and weed seeds and growth of shoot and root were reduced significantly in test treatments in comparison to the control. The seed germination, shoot length and root length were low at higher concentration. Complete inhibition of seed germination of Ageratum conyzoides, Galinsoga parviflora and Cyperus rotundus at 5 and 10% leaf extract of A.dubia was observed. Allelopathic effects were more pronounced with leaf extract than root or stem extract in most cases.The result indicated difference in allelopathic effect on crop seed and weed seed at higher concentrations. ECOPRINT 21: 23-30, 2014DOI: http://dx.doi.org/10.3126/eco.v21i0.11901

scholarly journals Allelopathic Impacts of an Agroforestry Tree Species (Streblus asper Lour.) on Seed Germination and Seedling Growth of Chickpea

2021 ◽  
Author(s):  
S. Oraon ◽  
S. Mondal
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Tree Species ◽  
Root Length ◽  
Inhibitory Effect ◽  
Seed Vigor ◽  
Shoot Length ◽  
Allelopathic Effect ◽  
Leaf Extracts ◽  
Streblus Asper

Background: Agroforestry might be a better strategy for sustainable land use and crop production. Allelopathic effect of Streblus asper Lour. (Moraceae) an agroforestry tree species was documented on chickpea (Cicer arietinum L.) seed germination and seedling growth. Methods: The present study was conducted to evaluate the allelopathic potentialities of different concentrations (0.125%, 0.25%, 0.5%, 1.0%, 2.5% and 5%) of aqueous leaf extracts of S. asper on seed germination and seedling growth in laboratory based experiments. The allelopathic potentialities were studied based on seed germination, germination rate, root length, shoot length, biomass, seed vigor index etc. Result: The inhibitory effect was more pronounced with the increasing concentrations of aqueous extracts. 5% aqueous leaf extracts showed a maximum inhibitory effect on seed germination, root length, shoot length, dry weight of root and shoot as compared to control. Based on the allelopathic index, concentrations of 5% aqueous extract showed a strong inhibitory effect. Aqueous leaf extracts contain water-soluble allelochemicals which affect the seed germination and seedling growth. The inhibitory allelopathic effect can be taken as serious consideration before plantation of chickpea in an association of this tree species because of its inhibitory effect on seed germination and early stages of development.

Inhibitory effect of aqueous extracts of Centaurea solstitialis subsp. schouwii on seed germination and growth of Sulla coronaria

Botany ◽  
2020 ◽  
Vol 98 (5) ◽  
pp. 273-281
Author(s):  
Chadlia Hachani ◽  
Mohammed S. Lamhamedi ◽  
Mejda Abassi ◽  
Zoubeir Béjaoui
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Inhibitory Effect ◽  
Centaurea Solstitialis ◽  
Aqueous Extracts ◽  
Linear Relationships ◽  
Allelopathic Effects ◽  
Anthropogenic Threats ◽  
Sulla Coronaria

Biodiversity has been confronted with anthropogenic threats and several natural threats such as biological invasions. The success of these invasions involves phytotoxic products released by invasive plants that can exhibit allelopathic effects on target species. Thus, aqueous extracts from different parts of the Mediterranean yellow star-thistle [Centaurea solstitialis subsp. schouwii (DC.) Gugler], were tested for their allelopathic effects on seed germination and seedling growth of Sulla coronaria (L.). Bioassays were conducted in vitro to test the effects of the aqueous extracts of shoot, basal and root parts of C. solstitialis subsp. schouwii at two different concentrations (50 g·L−1 and 10 g·L−1). The concentrations of total polyphenols, flavonoids, and tannins of the extracts were also evaluated. Our results showed inhibitory effects on the germination and seedling growth of S. coronaria seedlings, particularly with the extract form the basal part, reaching 84%. This study confirms the linear relationships between the allelopathic effects of C. solstitialis subsp. schouwii and the polyphenol and flavonoid contents. However, further experiments are needed under field conditions to confirm the results obtained under laboratory conditions.

scholarly journals Allelopathic Effect of Leaf Extract of Two Wild Plants on Seed Germination, Shoot and Root Lengths of Two Weed Species; Portulacaoleracea and Chenopodium murale

2018 ◽  
Vol 15 (4) ◽  
pp. 929-935 ◽  
Author(s):  
Nadi Awad Al-Harbi
Keyword(s):  
Seed Germination ◽  
Weed Management ◽  
Leaf Extract ◽  
Inhibitory Effect ◽  
Portulaca Oleracea ◽  
Wild Plants ◽  
Allelopathic Effect ◽  
Weed Species ◽  
Chenopodium Murale

The present study aims to evaluate the effect of leaf extract of Rhanterium epapposum and Salsola imbricata at various concentrations (20%, 40%, and 60%) on the germination and shoot and root lengths of two weed species; Portulaca oleracea and Chenopodium murale. The present study findings proved that the inhibitory effect of seed germination and shoot and root lengths of the studied species was largely dependent on the concentration of R. epapposum and S.imbricata leaf extract. The results showed that the highest allelopathic effect on the germination of the seeds of C.murale by leaf extract of R. epapposum and S. imbricata especially at concentration of 40% and 60% . While the lowest effect was of the leaf extract of S.imbricata. However the concentration of 60% was higher on the germination of the seeds C. murale and P. oleracea. Results also showed that the root and shoot lengths of C. murael was more sensitive to allelochemicals of R. epapposum leaf extract compared to S.imbricata leaf extract. However, the root and shoot lengths of P. oleracea was more sensitive to allelochemicals of S. imbricata leaf extract compared to R.epapposum leaf extract. Hence, the present findings recommended using the R. epapposum leaf extract and S. imbricata leaf extract as a tool for weed management especially C. murale and P. oleracea.

scholarly journals Allelopathic Effects of Cinnamomum migao on Seed Germination and Seedling Growth of its Associated Species Liquidambar formosana

Forests ◽  
2019 ◽  
Vol 10 (7) ◽  
pp. 535
Author(s):  
Deng Wang ◽  
Jingzhong Chen ◽  
Xue Xiong ◽  
Shu Wang ◽  
Jiming Liu
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Leaf Extract ◽  
Rhizosphere Soil ◽  
Starch Accumulation ◽  
Soil Extracts ◽  
Aqueous Leaf Extract ◽  
Allelopathic Effects ◽  
High Concentrations ◽  
Associated Species

We examined allelopathic effects and underlying mechanisms of Cinnamomum migao on its associated species Liquidambar formosana. We assessed effects of aqueous extracts of C. migao pericarp, leaf, and rhizosphere soil at different concentrations on seed germination, seedling growth, and physiology of L. formosana. All extracts inhibited L. formosana seed germination, with obvious inhibition at high concentrations (50 mg mL−1). All extracts promoted the height and ground diameter of seedlings, with the highest promotion achieved with aqueous leaf extract at a concentration of 1 mg mL−1 and aqueous pericarp and rhizosphere soil extracts at a concentration of 5 mg mL−1. All extracts promoted soluble protein accumulation in L. formosana seedlings, with the highest accumulation achieved with aqueous pericarp extracts. Aqueous leaf extract promoted soluble starch accumulation. Aqueous pericarp extract at concentrations of >10 mg mL−1 significantly increased soluble sugar content. Aqueous leaf and rhizosphere soil extracts at concentrations of >5 mg mL−1 increased proline accumulation. All extracts at concentrations of >1 mg mL−1 significantly increased malondialdehyde content. Aqueous pericarp and rhizosphere soil extracts at concentrations of 10 and 0.5 mg mL−1, respectively, promoted superoxide dismutase activity. Activities of soil urease, polyphenol oxidase, and catalase were significantly increased when the concentration of aqueous pericarp and leaf extracts exceeded 5 mg mL−1, and the activity of soil acid phosphatase significantly increased when the concentration of all extracts were 5 mg mL−1. According to the synthetic allelopathic index, the low- and medium-concentration extracts all showed a promoting effect, whereas high concentrations exhibited obvious inhibitory effects; furthermore, the comprehensive effect value of leaf water extraction was higher than that of the pericarp and rhizosphere soil. Thus, allelopathy can affect the long-term co-existence of C. migao and L. formosana.

scholarly journals PHYTOTOXIC ACTIVITY OF MORINGA OLEIFERA LEAF EXTRACT ON GERMINATION AND SEEDLING GROWTH OF TOMATO

2021 ◽  
Vol 21 (No 1) ◽  
Author(s):  
Ruchi Vyas ◽  
Kanika Sharma
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Crude Extract ◽  
Extraction Method ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Inhibitory Effect ◽  
Phytotoxic Activity ◽  
Young Leaves

The aim of this study was to evaluate the phytotoxic potential of 50% alcoholic crude extract as well as benzene fraction of young leaves of Moringa oleifera Both determine the growth bioassay of seed germination and seedling growth of tomato (Solanum lycopersicon). The crude extract was prepared by cold extraction method and benzene fraction was prepared by hot extraction method with soxhlet assembly. Extraction was carried out with healthy young leaves (in powder form) and organic solvent. Concentrations are used for phytotoxicity assay is 5,10,15,20 and 25mg/ml. Phytotoxicity was done by standard blotter method. The Result exhibited that 5, 10 and 15 mg/ml concentration of extract was non phytotoxic and were further used for in vivo experiments using chambered Thick Paper Glass. The phytotoxic potential of the crude as well as partially purified extract of leaves of Moringa oleifera varied according to concentration and it is called concentration depended inhibitory effect. The greatest inhibitory effect on the seed germination that is 37% in crude extract and 30% in a partially purified extract of 25mg/ml concentration of leaf extract. Equally, 25 mg/ml concentration detected to be toxic against a seedling growth of tomato and demonstrating that the compound responsible for the phytotoxic

scholarly journals Studies on Allelopathic Effect of Aqueous Leaf Extract of Putranjiva Roxburghii Wall. on Seed Germination and Early Growth of Chickpea (Cicer Arietinum L.)

2019 ◽  
Author(s):  
Satyajit Oraon ◽  
Subrata Mondal
Keyword(s):  
Seed Germination ◽  
Leaf Extract ◽  
Inhibitory Effect ◽  
Early Growth ◽  
Growth Stages ◽  
Allelopathic Effect ◽  
Leaf Extracts ◽  
Cicer Arietinum L ◽  
Aqueous Leaf Extract ◽  
Putranjiva Roxburghii

The present study reveals the allelopathic effect of aqueous leaf extracts of Putranjiva roxburghii Wall. on seed germination and early growth stages of an economically important plant chickpea (Cicer arietinum L.). Aqueous leaf extracts at 0, 5, 10, 15, 25, 35, 50, 75 and 100% concentrations were applied to determine their effect on seed germination and early growth stages under laboratory conditions. Laboratory-based experiments with three replicates were used to arrange treatments accordingly. After 5 days of incubation the rate of seed germination, radicle length and hypocotyls length etc. of chickpea seeds were observed after treatment with aqueous leaf extracts. Highest inhibitory effect was noticed in 100% of aqueous leaf extract. Results indicated that the inhibitory effect was proportional to the concentration of the extracts. The water soluble leaf extracts contain allelochemicals, which inhibit the seed germination and reduced the early growth of chickpea.

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