Purification of Pomegranate Pericarps (Punica granatum l.) Extracts with Macroporous Resins and Evaluation of Antioxidant Activities In Vivo

2014 ◽  
Vol 998-999 ◽  
pp. 296-301
Author(s):  
Ben Hong Zhou ◽  
Jie Tu ◽  
Jing Zhou ◽  
Hui Lan Yi ◽  
Yue Wu ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Erythrocyte Membrane ◽  
Uv Light ◽  
Antioxidant Properties ◽  
Punica Granatum ◽  
Membrane Lipid ◽  
Dependent Manner ◽  
Membrane Lipid Peroxidation ◽  
Microsomal Lipid Peroxidation ◽  
Punica Granatum L

Punica granatum L. Pomegranate has been traditionally used as a folk fruits for centuries in Oriental countries. It extract and subsequent fractions were used to evaluate antioxidant and related activities of Punica granatum L. It were extracted by 70% acetone, and then purified by HPD-400 macroporous adsorbent resins column. The aim of this study was to verify the antioxidant properties of acetone extracts of pomegranate pericarps and their lyophilized samples, and also to study the relationship between the activity and the contents of the tannins. The effects of natural extracts on microsomal lipid of mice liver and erythrocyte membrane lipid were examined. Vc/Fe2+, Cys/Fe2+induced microsomal lipid peroxidation, UV light induced erythrocyte membrane lipid peroxidation were measured by malondialdehyde (MDA) content with spectrophotometric methods. The natural plant extracts not only diminish the Vc/Fe2+,Cys/Fe2+ induced microsomal lipid peroxidation, UV light induced erythrocyte membrane lipid peroxidation and also showed its activity in a concentration-dependent manner. Our study found that the higher tannins contents showed more effective of antioxidant properties. Keywords: Punica granatum L.; liver microsome; antioxidant activity; Lipid peroxidation

Erythrocyte membrane lipid peroxidation and glycosylated hemoglobin in diabetes

Diabetes ◽  
1989 ◽  
Vol 38 (12) ◽  
pp. 1539-1543 ◽  
Author(s):  
S. K. Jain ◽  
R. McVie ◽  
J. Duett ◽  
J. J. Herbst
Keyword(s):  
Lipid Peroxidation ◽  
Erythrocyte Membrane ◽  
Glycosylated Hemoglobin ◽  
Membrane Lipid ◽  
Membrane Lipid Peroxidation

Erythrocyte Membrane Lipid Peroxidation and Glycosylated Hemoglobin in Diabetes

Diabetes ◽  
1989 ◽  
Vol 38 (12) ◽  
pp. 1539-1543 ◽  
Author(s):  
S. K. Jain ◽  
R. McVie ◽  
J. Duett ◽  
J. J. Herbst
Keyword(s):  
Lipid Peroxidation ◽  
Erythrocyte Membrane ◽  
Glycosylated Hemoglobin ◽  
Membrane Lipid ◽  
Membrane Lipid Peroxidation

Increased erythrocyte antioxidant status protects against smoking induced hemolysis in moderate smokers

2011 ◽  
Vol 30 (10) ◽  
pp. 1475-1481 ◽  
Author(s):  
Padmavathi Pannuru ◽  
Damodara Reddy Vaddi ◽  
Rameswara Reddy Kindinti ◽  
Nallanchakravarthula Varadacharyulu
Keyword(s):  
Lipid Peroxidation ◽  
Erythrocyte Membrane ◽  
Antioxidant Status ◽  
Reduced Glutathione ◽  
Membrane Lipid ◽  
Red Cell ◽  
Membrane Lipid Peroxidation ◽  
Free Radical Damage ◽  
Human Red Blood Cells ◽  
Nitrite Nitrate

Cigarette smoking is common in societies worldwide and has been identified as injurious to human health. Human red blood cells are important targets for electrophilic and oxidant foreign compounds. In the present study, the possible role of antioxidant status on smoking-induced erythrocyte hemolysis of smokers was studied. Erythrocyte superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, reduced glutathione (GSH) level, erythrocyte membrane lipid peroxidation, total cholesterol and phospholipids were determined. Further, nitrite/nitrate levels (NO2/NO3) in both plasma and erythrocyte lysate were measured. Results showed increased plasma and erythrocyte membrane lipid peroxidation and nitrite/nitrate levels in smokers. The activities of SOD, CAT and GPx were also increased with reduced glutathione (GSH) level in smokers. No significant change was observed in smokers red cell hemolysis and cholesterol/phospholipid (C/P) ratio compared to controls. Erythrocyte membrane lipid peroxidation was positively correlated with SOD ( r = 0.482, p < 0.01) and GPx ( r = 0.368, p < 0.018) in smokers. Increased levels of nitrite/nitrate and antioxidant status of erythrocytes might be playing a crucial role in protecting red cell from free radical damage induced by cigarette smoke.

Combined efficacies of lipoic acid and meso-2,3-dimercaptosuccinic acid on lead-induced erythrocyte membrane lipid peroxidation and antioxidant status in rats

2003 ◽  
Vol 22 (4) ◽  
pp. 183-192 ◽  
Author(s):  
R Sivaprasad ◽  
M Nagaraj ◽  
P Varalakshmi
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Body Weight ◽  
Erythrocyte Membrane ◽  
Lipoic Acid ◽  
Combined Treatment ◽  
Membrane Lipid ◽  
Dimercaptosuccinic Acid ◽  
Membrane Lipid Peroxidation ◽  
Antioxidant Defence System

One of the most intriguing phenomenon observed during lead toxicity has been attributed to lead-induced oxidative stress. The combined effect of DL-a-lipoic acid (LA) and meso 2,3-dimercaptosuccinic acid (DMSA) on lead-induced alterations in selected parameters, which are indicators of oxidative stress in erythrocytes, have been studied. Lead acetate (Pb, 0.2%) was administered in drinking water for 5 weeks to induce toxicity. LA (25 mg/ kg body weight per day i.p.) and DMSA (20 mg/kg body weight per day i.p.) were administered individually and also in combination during week 6. Clinical evidence of toxic exposure was evident from the elevated blood lead levels (BPb) along with lowered levels of haemoglobin (Hb) and haematocrit (Ht). Lead-exposed animals showed enhanced membrane lipid peroxidation (LPO) in the erythrocytes. Damage to the erythrocyte membrane was evident from the decline in the activities of the trans-membrane enzymes, viz., Na1, K1 ATPase, Ca21 ATPase and Mg21 ATPase. Lead-exposed rats also suffered an onslaught on the antioxidant defence system witnessed by lowered activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and reduced glutathione (GSH). Serum glutamic-oxoloacetic transaminase (SGOT) and serum glutamic-pyruvic transaminase (SGPT) were also elevated in lead-exposed rats. Treatment with either LA or DMSA reversed the lead-induced biochemical disturbances encountered by the erythrocytes, but combined treatment with LA and DMSA was very effective in mitigating all the parameters indicative of oxidative stress.

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