Biodegradable and Bioactive Polymer/Bioglass® Composite Foams for Tissue Engineering Scaffolds

2005 ◽  
Vol 494 ◽  
pp. 499-506 ◽  
Author(s):  
Aldo Roberto Boccaccini ◽  
J.J. Blaker ◽  
V. Maquet ◽  
R. Jerome ◽  
S. Blacher ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Chemical Reactivity ◽  
Poly Lactic Acid ◽  
Tissue Engineering Scaffolds ◽  
High Chemical ◽  
Thermally Induced ◽  
Composite Foams ◽  
45S5 Bioglass ◽  
Hydroxyapatite Crystals

Porous bioresorbable and bioactive composite materials designed for applications as scaffolds in tissue engineering are discussed. The systems fabricated by thermally induced phase separation method and based on poly(D,L-lactide) (PDLLA) or poly(lactic acid-co-glycolic acid) (PLGA) with additions of bioactive glass particles (45S5 Bioglass®) are described in detail. The scaffolds exhibit a well-defined, oriented and interconnected porosity. The porosity structure of foams with and without Bioglass® was characterised by scanning electron microscopy. The in vitro bioactivity and degradability of the composite foams were investigated in contact with phosphate buffer saline (PBS) and simulated body fluid (SBF). High chemical reactivity of scaffolds in SBF, which leads to the prompt formation of bonelike hydroxyapatite crystals on the material surfaces, indicates an enhanced bioactive character of the composites and therefore their potential for use as bone tissue engineering scaffolds.

scholarly journals Fabrication andIn VitroEvaluation of Nanosized Hydroxyapatite/Chitosan-Based Tissue Engineering Scaffolds

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Tao Sun ◽  
Tareef Hayat Khan ◽  
Naznin Sultana
Keyword(s):  
Tissue Engineering ◽  
Composite Scaffold ◽  
Three Dimensional ◽  
Biological Evaluation ◽  
Mouse Fibroblast ◽  
Tissue Engineering Scaffolds ◽  
Composite Scaffolds ◽  
Thermally Induced ◽  
Chitosan Composite

Composite scaffolds based on biodegradable natural polymer and osteoconductive hydroxyapatite (HA) nanoparticles can be promising for a variety of tissue engineering (TE) applications. This study addressed the fabrication of three-dimensional (3D) porous composite scaffolds composed of HA and chitosan fabricated via thermally induced phase separation and freeze-drying technique. The scaffolds produced were subsequently characterized in terms of microstructure, porosity, and mechanical property.In vitrodegradation andin vitrobiological evaluation were also investigated. The scaffolds were highly porous and had interconnected pore structures. The pore sizes ranged from several microns to a few hundred microns. The incorporated HA nanoparticles were well mixed and physically coexisted with chitosan in composite scaffold structures. The addition of 10% (w/w) HA nanoparticles to chitosan enhanced the compressive mechanical properties of composite scaffold compared to pure chitosan scaffold.In vitrodegradation results in phosphate buffered saline (PBS) showed slower uptake properties of composite scaffolds. Moreover, the scaffolds showed positive response to mouse fibroblast L929 cells attachment. Overall, the findings suggest that HA/chitosan composite scaffolds could be suitable for TE applications.

Preparation, Characterization and In Vitro Release Kinetics of Vancomycin Carried β-TCP/Chitosan Composite Microspheres Used as Bone Tissue Engineering Scaffolds

2012 ◽  
Vol 512-515 ◽  
pp. 1821-1825
Author(s):  
Lin Zhang ◽  
Xue Min Cui ◽  
Qing Feng Zan ◽  
Li Min Dong ◽  
Chen Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Release Kinetics ◽  
In Vitro Release ◽  
Cross Linking ◽  
Tissue Engineering Scaffolds ◽  
Composite Microspheres ◽  
Chitosan Composite ◽  
Vitro Release

A novel microsphere scaffolds composed of chitosan and β-TCP containing vancomycin was designed and prepared. The β-TCP/chitosan composite microspheres were prepared by solid-in-water-in-oil (s/w/o) emulsion cross-linking method with or without pre-cross-linking process. The mode of vancomycin maintaining in the β-TCP/chitosan composite microspheres was detected by Fourier transform infrared spectroscopy (FTIR). The in vitro release curve of vancomycin in simulated body fluid (SBF) was estimated. The results revealed that the pre-cross-linking prepared microspheres possessed higher loading efficiency (LE) and encapsulation efficiency (EE) especially decreasing the previous burst mass of vancomycin in incipient release. These composite microspheres got excellent sphere and well surface roughness in morphology. Vancomycin was encapsulated in composite microspheres through absorption and cross-linking. While in-vitro release curves illustrated that vancomycin release depond on diffusing firstly and then on the degradation ratio later. The microspheres loading with vancomycin would be to restore bone defect, meanwhile to inhibit bacterium proliferation. These bioactive, degradable composite microspheres have potential applications in 3D tissue engineering of bone and other tissues in vitro and in vivo.

Tissue Engineering Scaffolds Based on Photocured Dimethacrylate Polymers for in Vitro Optical Imaging

2006 ◽  
Vol 7 (6) ◽  
pp. 1751-1757 ◽  
Author(s):  
Forrest A. Landis ◽  
Jean S. Stephens ◽  
James A. Cooper ◽  
Marcus T. Cicerone ◽  
Sheng Lin-Gibson
Keyword(s):  
Tissue Engineering ◽  
Optical Imaging ◽  
Tissue Engineering Scaffolds

Mineralized Nanofibers for Bone Tissue Engineering

2018 ◽  
pp. 461-475 ◽  
Author(s):  
Ozan Karaman
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Bone Grafts ◽  
Tissue Engineering Scaffolds ◽  
Promising Alternative ◽  
Biomimetic Scaffolds

The limitation of orthopedic fractures and large bone defects treatments has brought the focus on fabricating bone grafts that could enhance ostegenesis and vascularization in-vitro. Developing biomimetic materials such as mineralized nanofibers that can provide three-dimensional templates of the natural bone extracellular-matrix is one of the most promising alternative for bone regeneration. Understanding the interactions between the structure of the scaffolds and cells and therefore the control cellular pathways are critical for developing functional bone grafts. In order to enhance bone regeneration, the engineered scaffold needs to mimic the characteristics of composite bone ECM. This chapter reviews the fabrication of and fabrication techniques for fabricating biomimetic bone tissue engineering scaffolds. In addition, the chapter covers design criteria for developing the scaffolds and examples of enhanced osteogenic differentiation outcomes by fabricating biomimetic scaffolds.

scholarly journals Development of 3D Bioactive Nanocomposite Scaffolds Made from Gelatin and Nano Bioactive Glass for Biomedical Applications

2010 ◽  
Vol 19 (2) ◽  
pp. 096369351001900 ◽  
Author(s):  
M. Mozafari ◽  
F. Moztarzadeh ◽  
M. Rabiee ◽  
M. Azami ◽  
N. Nezafati ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Biomedical Applications ◽  
Cell Attachment ◽  
Tissue Engineering Scaffolds ◽  
Study Results ◽  
Nanocomposite Scaffold ◽  
Nanocomposite Scaffolds ◽  
First Time

In this research, macroporous, mechanically competent and bioactive nanocomposite scaffolds have been fabricated from cross-linked gelatine (Gel) and nano bioactive glass (nBG) through layer solvent casting combined with freeze-drying and lamination techniques. This study has developed a new composition to produce a new bioactive nanocomposite which is porous with interconnected microstructure, pore sizes are 200-500 μm, porosity are 72%-86%. Also, we have reported formation of chemical bonds between nBG and Gel for the first time. Finally, the in vitro cytocompatability of the scaffolds was assessed using MTT assay and cell attachment study. Results indicated no sign of toxicity and cells found to be attached to the pore walls offered by the scaffolds. These results suggested that the developed nanocomposite scaffold possess the prerequisites for bone tissue engineering scaffolds and it can be used for tissue engineering applications.

scholarly journals Feasibility of Spatially Offset Raman Spectroscopy for in Vitro and in Vivo Monitoring Mineralization of Bone Tissue Engineering Scaffolds

2016 ◽  
Vol 89 (1) ◽  
pp. 847-853 ◽  
Author(s):  
Zhiyu Liao ◽  
Faris Sinjab ◽  
Amy Nommeots-Nomm ◽  
Julian Jones ◽  
Laura Ruiz-Cantu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Raman Spectroscopy ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tissue Engineering Scaffolds ◽  
In Vivo Monitoring
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