Paraoxonase 1 phenotype distribution in a cohort of healthy Sri Lankan population

Background:In this study, we aimed to evaluate the association between hydatidiform mole (HM) and paraoxonase 1 (PON1) activity and phenotyping in comparison with healthy subjects (both pregnant and nonpregnant women).Methods:Eighty women were enrolled in this study. Of these, 26 had HM (group 1). Twenty-seven healthy women in the first trimester of pregnancy with a single viable fetus were included in the study as control subjects (group 2). The remaining 27 subjects were healthy nonpregnant women who also participated as control subjects (group 3). Paraoxonase and arylesterase activities were measured using commercially available kits (Relassay, Gaziantep, Turkey). Paraoxonase activity measurements were performed both in the absence (basal activity) and presence of NaCl (salt-stimulated activity). The phenotype distribution of PON1 activity was determined by the double-substrate method.Results:Basal and salt-stimulated paraoxonase and arylesterase activities were not significantly different between the HM, healthy pregnant control, and healthy nonpregnant control groups. Hence, PON1 phenotype distribution of the subjects was not significantly different between these 3 groups.Conclusions:We determined no association between HM (complete or partial) and PON1 activity and phenotyping in comparison with pregnant and nonpregnant healthy subjects. Further studies including metastatic or invasive HM are needed.

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The Phenotype Distribution of Paraoxonase-1 in Patients with Multiple Myeloma, Bladder, and Colorectal Cancer

Journal of Medical Biochemistry ◽

10.2478/jomb-2014-0011 ◽

2014 ◽

Vol 33 (3) ◽

pp. 252-258 ◽

Cited By ~ 4

Author(s):

Hamit Yasar Ellidag ◽

Esin Eren ◽

Ozgur Aydin ◽

Salim Neselioglu ◽

Necat Yilmaz

Keyword(s):

Colorectal Cancer ◽

Multiple Myeloma ◽

Healthy Subjects ◽

Paraoxonase 1 ◽

Control Group ◽

Control Groups ◽

Phenotype Distribution ◽

Serum Paraoxonase ◽

And Control ◽

Dual Substrate

Abstract Background: Human serum paraoxonase-1 (PON1) shows wide variation among different ethnic groups around the world. The aim of the present study was to determine the phenotype distribution and enzymatic activity of PON1 and ARE (arylesterase) in colorectal cancer (CRC), bladder cancer (BC) and multiple myeloma (MM) patients compared to healthy subjects. Methods: A total of 160 subjects (40 CRC patients, 40 BC patients, 40 MM patients and 40 healthy controls) were admitted to the study. The phenotype distribution of PON1 was determined by using the dual substrate (paraoxon and phenylacetate) method. Results: PON 1 and ARE activities were significantly lower in the cancer patients compared to the control group. The following phenotype distributions were assessed in the cancer and control groups: MM: 52.5% (QQ), 40% (QR), 7.5% (RR); CRC: 52.5% (QQ), 40% (QR), 7.5% (RR); BC: 55% (QQ), 35% (QR), 10% (RR); and controls: 40% (QQ), 57.5% (QR), 2.5% (RR). Conclusions: We found that MM, CRC and BC patients were associated with lower PON1, ARE and stPON1 enzyme activities compared to the healthy subjects. However, PON1 phenotypes were similar between the cancer groups and control group.

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Paraoxonase 1 phenotype distribution and activity differs in subjects with newly diagnosed Type 2 diabetes (the CODAM Study)

Diabetic Medicine ◽

10.1111/j.1464-5491.2007.02328.x ◽

2008 ◽

Vol 25 (2) ◽

pp. 186-193 ◽

Cited By ~ 21

Author(s):

S. W. van den Berg ◽

E. H. J. Jansen ◽

M. Kruijshoop ◽

P. K. Beekhof ◽

E. Blaak ◽

...

Keyword(s):

Type 2 Diabetes ◽

Paraoxonase 1 ◽

Newly Diagnosed ◽

Phenotype Distribution

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W06.172 Paraoxonase 1 (PON1) enhances HDL-mediated macrophage cholesterol efflux via the ABCA1 transporter: A possible role for lysophosphatidylcholine

Atherosclerosis ◽

10.1016/s0021-9150(04)90171-2 ◽

2004 ◽

Vol 5 (1) ◽

pp. 40

Author(s):

M ROSENBLAT

Keyword(s):

Cholesterol Efflux ◽

Paraoxonase 1 ◽

Macrophage Cholesterol Efflux

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Homocysteine thiolactonase activity of paraoxonase 1 in heart failure patients

European Journal of Heart Failure Supplements ◽

10.1016/s1567-4215(08)60399-9 ◽

2008 ◽

Vol 7 ◽

pp. 141-141

Author(s):

E MOLDOVEANU ◽

D MARTA ◽

C TANASEANU ◽

M SERBAN ◽

I SERBAN ◽

...

Keyword(s):

Heart Failure ◽

Paraoxonase 1 ◽

Heart Failure Patients ◽

Homocysteine Thiolactonase

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Paraoxonase-1 and myeloperoxidase correlate with vascular biomarkers in overweight patients with newly diagnosed untreated hyperlipidaemia

VASA ◽

10.1024/0301-1526/a000643 ◽

2017 ◽

Vol 46 (5) ◽

pp. 370-376 ◽

Cited By ~ 2

Author(s):

Anita Szentpéteri ◽

Noémi Zsíros ◽

Viktória E. Varga ◽

Hajnalka Lőrincz ◽

Mónika Katkó ◽

...

Keyword(s):

Adhesion Molecule ◽

Oxidized Ldl ◽

Vascular Complications ◽

Cd40 Ligand ◽

Paraoxonase 1 ◽

Intercellular Adhesion Molecule ◽

Pon1 Activity ◽

Intercellular Adhesion Molecule 1 ◽

Inflammatory Parameters ◽

Vascular Biomarkers

Abstract. Background: In hyperlipidaemic state, increased levels of myeloperoxidase (MPO) and decreased paraoxonase-1 (PON1) activity have been reported; however, their relationships with other atherosclerotic biomarkers have not been completely clarified. Patients and methods: Serum concentrations of lipid and inflammatory parameters, MPO levels, and PON1 activities were investigated in 167 untreated hyperlipidaemic patients with and without vascular complications and in 32 healthy controls. Additionally, levels of CD40 ligand (sCD40L) and asymmetric dimethyl arginine (ADMA), soluble intercellular adhesion molecule-1 (sICAM-1), soluble vascular cell adhesion molecule-1, and oxidized LDL were determined. Results: We found elevated C-reactive protein (CRP), ADMA, sCD40L, sICAM-1 concentrations, and higher MPO levels in patients with vascular complications compared to those without. The PON1 arylesterase activity correlated negatively with sCD40L, ADMA, and sICAM-1 levels, respectively. In contrast, MPO concentrations showed positive correlations with sCD40L, ADMA, and sICAM-1 levels, respectively. Conclusions: It can therefore be stated that PON1 activity and MPO level correlate strongly with the vascular biomarkers, highlighting the importance of the HDL-associated pro- and antioxidant enzymes in the development of endothelial dysfunction and atherogenesis.

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