TREHALASE ACTIVITY IN THE SILK GLANDS OF THE SILKWORM, BOMBYX MORI (LEPIDOPTERA: BOMBYCIDAE)

1975 ◽  
Vol 107 (12) ◽  
pp. 1311-1314 ◽  
Author(s):  
Shuya Shimada
Keyword(s):  
Bombyx Mori ◽  
Divalent Cations ◽  
Silk Gland ◽  
Trehalase Activity ◽  
Gland Cells ◽  
Silk Glands ◽  
Tunica Propria ◽  
Silkworm Bombyx Mori ◽  
Cu And Zn ◽  
Tunica Intima

Abstract(1) Crude extracts prepared from the silk glands of the silkworm, Bombyx mori L. contain trehalase activity. (2) Trehalase in the silk glands has a pH of 5.5 and a Km of 0.71 mM. The activity of the enzyme is inhibited by divalent cations such as Mn, Cu, and Zn. (3) By histochemical methods, it is shown that trehalase is localized in the periphery of the silk gland cells, especially in the tunica propria and tunica intima. (4) Trehalase activity is low in fifth instar and increases greatly in spinning stages, after which the activity decreases.

scholarly journals EXTRUSION OF NUCLEAR MATERIALS INTO CYTOPLASM IN THE POSTERIOR SILK GLAND CELLS OF SILKWORM, BOMBYX MORI

1968 ◽  
Vol 36 (3) ◽  
pp. C5-10 ◽  
Author(s):  
Yutaka Tashiro ◽  
Shiro Matsuura ◽  
Takashi Morimoto ◽  
Sunao Nagata
Keyword(s):  
Bombyx Mori ◽  
Silk Gland ◽  
Nuclear Materials ◽  
Gland Cells ◽  
Silkworm Bombyx Mori ◽  
Posterior Silk Gland

Autophagy, apoptosis, and ecdysis-related gene expression in the silk gland of the silkworm (Bombyx mori) during metamorphosis

2010 ◽  
Vol 88 (12) ◽  
pp. 1169-1178 ◽  
Author(s):  
Qingrong Li ◽  
Xiaojuan Deng ◽  
Wanying Yang ◽  
Zhijun Huang ◽  
Gianluca Tettamanti ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Receptor Gene ◽  
Silk Gland ◽  
Apoptotic Bodies ◽  
Gland Cells ◽  
Cytoplasmic Material ◽  
Transcription Factor Genes ◽  
Insect Metamorphosis ◽  
Apoptosis Pathways ◽  
Silkworm Bombyx Mori

Degeneration of larval-specific tissues during insect metamorphosis has been suggested to be the result of apoptosis and autophagy and is triggered by ecdysteroids. However, the relationship between autophagy and apoptosis pathways and the mechanism of regulation by ecdysteroids remain to be elucidated. This study examined the events of autophagy, apoptosis, and the expression of ecdysis-related genes in the silk gland of the silkworm ( Bombyx mori L., 1758) during the larval to pupal transformation. The results indicated that autophagic features appeared in the silk gland at the wandering and spinning stages of the larvae, whereas the apoptotic features such as apoptotic bodies and DNA fragmentation occurred at the prepupal or early-pupal stages. The autophagic granules fused with each other to form large vacuoles where the cytoplasmic material was degraded. Autophagosomes, autolysosomes, and apoptotic bodies were found later in the degenerating silk-gland cells. Expression of the ecdysone receptor gene BmEcR and the transcription factor genes BmE74A and BmBR-C preceded the onset of autophagy and apoptosis, indicating that they may be responsible for triggering these programmed cell death pathways in the silk gland. The results suggest that both autophagy and apoptosis occur in the silk-gland cells during degeneration, but autophagy precedes apoptosis.

Effects of starvation and hormones on DNA synthesis in silk gland cells of the silkworm,Bombyx mori

2015 ◽  
Vol 23 (4) ◽  
pp. 569-578 ◽  
Author(s):  
Yao-Feng Li ◽  
Xiang-Yun Chen ◽  
Chun-Dong Zhang ◽  
Xiao-Fang Tang ◽  
La Wang ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Bombyx Mori ◽  
Silk Gland ◽  
Gland Cells ◽  
Silkworm Bombyx Mori

Expression of autophagy-related genes in the anterior silk gland of the silkworm (Bombyx mori) during metamorphosis

2011 ◽  
Vol 89 (11) ◽  
pp. 1019-1026 ◽  
Author(s):  
Qingrong Li ◽  
Xiaojuan Deng ◽  
Zhijun Huang ◽  
Sichun Zheng ◽  
Gianluca Tettamanti ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Morphological Characteristics ◽  
Time Frame ◽  
Silk Gland ◽  
Marker Genes ◽  
Reverse Transcription Pcr ◽  
Evolutionarily Conserved ◽  
Silk Glands ◽  
Autophagic Process ◽  
Silkworm Bombyx Mori

Programmed cell death (PCD) is a genetically regulated process of cell elimination and is evolutionarily conserved in eukaryotes. Degeneration of larval tissues during metamorphosis in insects is a result of PCD triggered by ecdysteroids and autophagic process has been shown to be involved in the degeneration of silk gland of the silkworm ( Bombyx mori L., 1758). However, experimental evidence for the expression of autophagy marker genes remains insufficient. In this study, expression of the autophagy-related genes BmAtg5, BmAtg6, BmAtg8, and BmAtg12 was determined in the anterior silk glands of larvae and pupae during larval to pupal transformation by using reverse-transcription PCR, quantitative real-time PCR, and Western blot analyses. The results indicated that BmAtg5, BmAtg6, BmAtg8, and BmAtg12 had the highest expression levels on the 3rd day of the wandering stage or at the prepupal stage, and the time frame of the expression of these genes was coincident with the morphological characteristics of autophagy in the silk gland during the larval to pupal transformation. This study demonstrated that the autophagy-related genes are involved in the degeneration of the anterior silk gland of B. mori.

Polytene chromosomes in silk gland cells of the silkworm,Bombyx mori

1969 ◽  
Vol 25 (4) ◽  
pp. 384-385 ◽  
Author(s):  
Y. H. Nakanishi ◽  
H. Kato ◽  
S. Utsumi
Keyword(s):  
Bombyx Mori ◽  
Polytene Chromosomes ◽  
Silk Gland ◽  
Gland Cells ◽  
Silkworm Bombyx Mori
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