Effect of Dexamethasone on Gene Expression of Surfactant Protein B and Surfactant Protein C

2003 ◽  
Vol 54 (4) ◽  
pp. 439
Author(s):  
Ik Soo Park ◽  
Jang Won Sohn ◽  
Ho Joo Yoon ◽  
Dong Ho Shin ◽  
Sung Soo Park
Keyword(s):  
Gene Expression ◽  
Protein C ◽  
Surfactant Protein ◽  
Surfactant Protein C ◽  
Surfactant Protein B ◽  
Protein B

Rabbit surfactant protein C: cDNA cloning and regulation of alternatively spliced surfactant protein C mRNAs

1992 ◽  
Vol 263 (6) ◽  
pp. L634-L644 ◽  
Author(s):  
V. Boggaram ◽  
R. K. Margana
Keyword(s):  
Gene Expression ◽  
Lung Development ◽  
Genomic Dna ◽  
Protein C ◽  
Fetal Lung ◽  
Surfactant Protein ◽  
Surfactant Protein C ◽  
Fetal Lung Development ◽  
Mrna Content

Surfactant protein C (SP-C), a hydrophobic protein of pulmonary surfactant is essential for surfactant function. Toward elucidating molecular mechanisms that mediate regulation of SP-C gene expression in rabbit lung, we isolated and characterized cDNAs encoding rabbit SP-C and studied the regulation of SP-C gene expression during fetal lung development and by adenosine 3',5'-cyclic monophosphate (cAMP) and dexamethasone in fetal lung tissues in vitro. We found that rabbit SP-C is highly homologous to SP-C of other species and is encoded by two mRNAs that differ by an insertion of 31 nucleotides in the 3' untranslated regions. SP-C mRNAs were classified into two types based on the nucleotide sequence; type I represents RNA without the 31 nucleotide insert and comprises approximately 80–90% of total SP-C mRNA content, whereas type II represents RNA containing the insert and comprises approximately 10–20% of total SP-C mRNA content. SP-C mRNAs were induced in a coordinate manner during fetal lung development and by cAMP and dexamethasone in fetal lung tissues in vitro. Southern hybridization analysis of genomic DNA suggested that SP-C mRNAs are encoded by a single gene. Polymerase [corrected] chain reaction-amplification of genomic DNA with oligonucleotide primers flanking the insertional sequence and sequence analysis of amplified DNA showed that SP-C mRNAs are produced by alternative use of 3' splice sites of intron 5 of SP-C gene.

Surfactant Protein B Detection and Gene Expression in Chronic Rhinosinusitis

2007 ◽  
Vol 117 (7) ◽  
pp. 1296-1301 ◽  
Author(s):  
Bradford A. Woodworth ◽  
Rachel Wood ◽  
Geeta Bhargave ◽  
Noam A. Cohen ◽  
John E. Baatz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chronic Rhinosinusitis ◽  
Surfactant Protein ◽  
Surfactant Protein B ◽  
Protein B
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