First report of Erysiphe convolvuli on Convolvulus arvensis and Calystegia sepium in Chile with a description of the sexual state

Field bindweed (Convolvulus arvensis L.; Convolvulaceae) is a troublesome perennial weed found among many important crops in the world (1). In May of 2007, dying field bindweed plants were found along the edge of a wheat (Triticum aestivum L.) field between Bafra and Taflan, Turkey (41°34.395′N, 35°52.215′E). Lesions on leaves were irregular and variable in size and dark black with green margins. Severely diseased leaves were wilted or dead. Fruiting bodies were not evident on field-collected material. Diseased tissue was surface disinfested and placed on moist filter paper in petri plates. Numerous pycnidia with alpha conidia were observed after 2 weeks. A fungus, designated 24-6, was isolated from the diseased leaves. Cultures on potato dextrose agar (PDA) were floccose with white mycelia and small black stromata. Alpha conidia from pycnidia on inoculated plants were biguttulate, one celled, hyaline, oblong to ellipsoid, and 7.0 to 12.8 × 3.0 to 5.5 μm (mean 10.0 × 3.9 μm). Neither beta conidia nor the teleomorph, Diaporthe sp., were observed on diseased tissue or in cultures. Morphology was consistent with that of Phomopsis convolvuli Ormeno-Nunez, Reeleder & A.K. Watson (2). Alpha conidia were harvested from 12-day-old cultures grown on PDA by brushing the surface of the colonies with a small paint brush, suspending the conidia in sterile distilled water, and filtering through cheesecloth. The conidia were then resuspended in sterile distilled water plus 0.1% polysorbate 20 to arrive at a concentration of 107 conidia/ml. Stems and leaves of seven plants at the 3- to 5-leaf stage were spray inoculated with 10 ml per plant of this aqueous suspension. Inoculated plants and two noninoculated plants were placed in a dew chamber at 24°C in darkness and continuous dew. After 48 h, plants from the dew chamber were moved to a greenhouse bench. Disease severity was evaluated 1 week after inoculation with a rating system based on a scale from 0 to 4, in which 0 = no symptoms, 1 = 1 to 25% necrosis, 2 = 26 to 50% necrosis, 3 = 51 to 75% necrosis, and 4 = 76 to 100% necrosis (2). The average disease rating on inoculated plants was 3.75. No disease was observed on noninoculated plants. P. convolvuli was reisolated from all inoculated plants. Comparison of the internal transcribed spacer (ITS) 1 and 2 sequences with available sequences of a vouchered P. convolvuli specimen (GenBank Nos. U11363, U11417; BPI 748009, FAU649) showed 192 of 193 and 176 of 179 identities, respectively, for the two regions. Nucleotide sequences for the ribosomal ITS regions (ITS 1 and 2, including 5.8S rDNA) were deposited in GenBank (Accession No. FJ710810), and a voucher specimen has been deposited with the U.S. National Fungus Collections (BPI 878927). To our knowledge, this is the second report in the world of leaf anthracnose on field bindweed caused by P. convolvuli. The first report was from Canada (3) of an isolate that was later patented for biological control of C. arvensis (4). References: (1) L. Holm et al. The World's Worst Weeds. University Press of Hawaii, Honolulu, 1977. (2) J. Ormeno-Nunez, et al. Can. J. Bot. 66:2228, 1988. (3) J. Ormeno-Nunez et al. Plant Dis. 72:338, 1988. (4) A. K. Watson et al. U.S. Patent 5,212,086, 1993.

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Hyalesthes obsoletus is an active vector of Wood blackening in the Republic of Moldova

BIO Web of Conferences ◽

10.1051/bioconf/20213404020 ◽

2021 ◽

Vol 34 ◽

pp. 04020

Author(s):

Evgeniy Haustov ◽

Victor Bondarciuc

Keyword(s):

Climatic Conditions ◽

Xanthium Strumarium ◽

Convolvulus Arvensis ◽

Bois Noir ◽

Republic Of Moldova ◽

Sonchus Oleraceus ◽

Field Bindweed ◽

Calystegia Sepium ◽

The Republic ◽

Hyalesthes Obsoletus

Hyalesthes obsoletus (Signoret) is an important vector of Wood Blackening in the Republic of Moldova. To identify the causes of the epidemiology of this disease in vineyards, the period of flight and the preference of the host plant H. obsoletus in the field were studied. Monitoring of vineyards showed that this species was found on the Field Bindweed (Convolvulus arvensis L.), and during the period of mass flight, the leafhopper was observed on Xanthium strumarium and Gorets bindweed (Fallopia convolvulus L.). The captured leafhoppers were diagnosed with the presence of Bois Noir stolbur phytoplasm (STOL). The adults of H. obsoletus were also caught from other herbaceous plants: Povoy fence (Calystegia sepium L.), Garden quinoa (Ariplex hortensis L.), Sow thistle (Sonchus oleraceus L.), Medicinal dandelion (Taraxacum officinale L.), as well as c Stinging nettle (Urtica dioica L.). Changes in climatic conditions in the region, causing premature drying of the grass cover, forces cicadas - vectors to switch to actively growing plants during this period, including grapes, which contributes to the widespread of the disease.

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Fusicladium convolvularum. [Descriptions of Fungi and Bacteria].

IMI Descriptions of Fungi and Bacteria ◽

10.1079/dfb/20056401513 ◽

2002 ◽

Author(s):

K. Schubert

Keyword(s):

Czech Republic ◽

New Zealand ◽

Great Britain ◽

Geographical Distribution ◽

Leaf Spot ◽

Convolvulus Arvensis ◽

Airborne Conidia ◽

Calystegia Sepium

Abstract A description is provided for Fusicladium convolvularum. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. DISEASE: Leaf spot. HOSTS: Species of Calystegia and Convolvulus (Convolvulaceae), including Calystegia sepium, C. soldanella and Convolvulus arvensis. GEOGRAPHICAL DISTRIBUTION: AUSTRALASIA: New Zealand. EUROPE: Czech Republic, Great Britain. TRANSMISSION: Presumably by airborne conidia.

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First report of a sexual state in an ambrosia fungus: Ambrosiella cleistominuta sp. nov. associated with the ambrosia beetle Anisandrus maiche

Botany ◽

10.1139/cjb-2016-0297 ◽

2017 ◽

Vol 95 (5) ◽

pp. 503-512 ◽

Cited By ~ 6

Author(s):

Chase G. Mayers ◽

Thomas C. Harrington ◽

Christopher M. Ranger

Keyword(s):

Dna Sequences ◽

Morphological Characters ◽

Its Rdna ◽

Ambrosia Beetle ◽

Ambrosia Beetles ◽

First Report ◽

The Usa ◽

Sexual State

Genera of ambrosia beetles in the tribe Xyleborini with large, mesonotal mycangia host unique fungal symbionts in the genus Ambrosiella. The symbiont of a recent invasive to the USA from Asia, Anisandrus maiche Stark, had not been previously characterized. We found the mycangium anatomy of An. maiche collected in Ohio to be similar to that of Anisandrus dispar and consistently isolated a novel fungus, Ambrosiella cleistominuta sp. nov., from An. maiche mycangia and galleries. The fungus was distinguished from other named Ambrosiella by morphological characters and DNA sequences (ITS rDNA and tef-1α). The mycangial symbionts of ambrosia beetles had been assumed to be strictly asexual, but A. cleistominuta produces cleistothecious ascomata with ascospores in beetle galleries and in culture. In contrast to ascomata of other Ceratocystidaceae, the relatively small ascomata of A. cleistominuta are neckless and without ostioles. The ascospores are relatively large, and single-ascospore colonies produced ascomata and ascospores in culture, showing that A. cleistominuta is homothallic.

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First report ofMycosphaerella graminicola, the sexual state ofSeptoria tritici, in Manitoba, Canada

Canadian Journal of Plant Pathology ◽

10.1080/07060660209507032 ◽

2002 ◽

Vol 24 (4) ◽

pp. 445-449 ◽

Cited By ~ 5

Author(s):

C. Hoorne ◽

L. Lamari ◽

G. M. Ballance ◽

J. Gilbert

Keyword(s):

First Report ◽

Sexual State

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Septoria convolvuli. [Descriptions of Fungi and Bacteria].

IMI Descriptions of Fungi and Bacteria ◽

10.1079/dfb/20056401412 ◽

1999 ◽

Author(s):

T. V. Andrianova

Keyword(s):

Geographical Distribution ◽

Tamil Nadu ◽

Infected Plant ◽

Far East ◽

Republic Of Georgia ◽

Plant Debris ◽

East Siberia ◽

Convolvulus Arvensis ◽

Ipomoea Tricolor ◽

Calystegia Sepium

Abstract A description is provided for Septoria convolvuli. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. DISEASE: Leaf spot. HOSTS: Calystegia divaricata, C. hederacea, C. inflata (syn. : Convolvulus sepium, Calystegia sepium), C. pseudocanthabrica, C. rosae, C. silvatica, C. soldanella, Convolvulus arvensis, C. calvertii, C. persicus, Ipomoea tricolor, Ipomoea sp. (Convolvulaceae). [Type host - Convolvulus arvensis.] GEOGRAPHICAL DISTRIBUTION: Asia: Armenia, Azerbaijan, China, Republic of Georgia, India (Tamil Nadu), Kazakhstan, Kirghizistan, Korea, Russia (East Siberia, Far East), Turkmenistan, Uzbekistan. Europe: Belgium, Bulgaria, Czech Republic, Denmark, Estonia, Germany, Ireland, Italy, Hungary, Latvia, Lithuania, Moldova, Norway, Poland, Portugal, Romania, Russia, UK, Ukraine, former Yugoslavia. North America: Canada, USA. South America: Argentina. TRANSMISSION: Not reported, but almost certainly by air-borne or splash dispersed conidia from infected plant débris.

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First Report of Candidatus Phytoplasma solani on Blackberry (Rubus fruticosus) in Bulgaria

Plant Disease ◽

10.1094/pdis-08-12-0793-pdn ◽

2013 ◽

Vol 97 (2) ◽

pp. 282-282 ◽

Cited By ~ 1

Author(s):

S. G. Bobev ◽

K. De Jonghe ◽

M. Maes

Keyword(s):

Consensus Sequence ◽

Elongation Factor ◽

Rflp Analysis ◽

Pcr Primers ◽

Convolvulus Arvensis ◽

First Report ◽

Severe Stunting ◽

Tuf Gene ◽

Specific Pcr ◽

Rubus Fruticosus

While performing a routine field survey on 2-year-old canes of Rubus fruticosus (cv. Evergreen Thornless) in the region of Plovdiv (central southern Bulgaria), severe stunting of single or grouped plants (3 to 4 in a row) was found in late August of 2009. It was noteworthy that the leaves of these plants were curved upwards and stayed green until the end of the season. The bushy aspect of the diseased plants led to the assumption of a phytoplasma origin; therefore, specific PCR and sequence based identification methods were applied on leaves, petioles, and stems from three infected Rubus plants grown in different rows of the field (midsummer, nine samples in total) and the same number of asymptomatic samples. Partial amplification of the 16S ribosomal RNA gene with generic phytoplasma primer pairs P1/P7 and fu5/ru3 (3), followed by a nested PCR specific for all members of the Phytoplasma stolbur subgroup by means of the stol11 primers (1), and an RFLP analysis of the tuf gene (elongation factor Tu) fragment produced with PCR primers tufAY/r tufAY (3), were used for the identification and characterization of the pathogen. All target amplicons were also sequenced by Macrogen (Seoul, South Korea) following gel purification (Nucleospoin Plant II, Macher-Nagel). Identical sequences were obtained from each of the P1/P7-derived amplicons (100% homology between samples) and a consensus 1,142 bp sequence was delineated and submitted to NCBI GenBank with accession no. JF293091. It had the highest similarity (99 to 100%) to sequences of ‘Bois noir’ phytoplasma (e.g. HQ589193; Candidatus Phytoplasma solani, position 29 to 1,171). The fu5/ru3 amplicons produced sequences that showed 99.5% homology to the Ca. Phytoplasma solani strains of a southern Russian and Romanian phytoplasma survey on different hosts (potato, tomato, Convolvulus) (GenBank Accession No. HM449999 to HM4450002). The stolbur specific primers also produced an amplicon in all samples and again the consensus sequence was identified (100% homology between the samples) and deposited in GenBank (JN561701). RFLP analysis of the tuf gene with the enzymes HindIII, HinfI, HpaII, and TaqI (Fermentas) produced the same profile types for the different samples and clearly allocated the phytoplasma in the tuf type-b (VKII), according to (2). This type is commonly reported as associated with bindweed (Convolvulus arvensis). Additionally, the sequenced tufAY fragment also confirmed a 100% correspondence with the submitted Tu elongation factor fragments of Ca. Phytoplasma solani strains in GenBank. No phytoplasma was detected in symptomless blackberry plants that were sampled from the same plot. In the molecular identification tests, a stolbur phytoplasma control (potato isolate), a Rubus stunt (EY subgroup, 16SrV) and an apple proliferation phytoplasma (AP subgroup, 16SrX) were used as controls. Based on the symptoms and the laboratory results, we concluded that the Rubus plants were infected by Ca. Phytoplasma solani, a species belonging to the stolbur subgroup (16SrXII-A). To our knowledge, this is the first report of Ca. Phytoplasma solani on Rubus fruticosus in Bulgaria. The disease is not likely to be an isolated case in the future because of the pathogen's spread on other hosts and the expected increase in blackberry fields. References: (1) X. Daire et al. Eur. J. Plant Pathol. 103:507, 1997. (2) M. Langer and M. Maixner. Vitis 43:191, 2004. (3) K.-H. Lorenz et al. Phytopathology 85:771, 1995.

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First report of field bindweed (Convolvulus arvensis L.) penetrates the roots of pepper (Capsicum annuum L.) plants.

10.35841/2591-7897.2.4.7-11 ◽

2018 ◽

Vol 02 (04) ◽

Author(s):

F.M. Farag

Keyword(s):

Capsicum Annuum ◽

Convolvulus Arvensis ◽

First Report ◽

Capsicum Annuum L ◽

Field Bindweed

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