Antioxidant Activities of Sonchus oleraceus L.

<p>Supernumerary free radicals and other reactive species can cause oxidative damage in animal cells, potentially leading to non-infectious diseases. Diets rich in low molecular weight antioxidants (LMWAs) may prevent or arrest the pathogenesis of these diseases. Leaves of Sonchus oleraceus L. may be an excellent dietary LMWA source for humans given their apparent strong antioxidant activities in vitro. However, different S. oleraceus plants vary in their antioxidant capacity. Nothing is known of possible environmental effects on antioxidant potential. Equally, the effects of cooking and gastrointestinal digestion are unknown. The goals of this research were: (i) to study the effects of plant age, locality, and abiotic stressors on antioxidant potential; (ii) to study the effects of cooking and in vitro gastrointestinal digestion on antioxidant activity and uptake in human cells; and (iii) to study extractable antioxidant activities of S. oleraceus cell suspension cultures in relation to abiotic stressors. Antioxidant activities and levels of total phenolics, hydroxycinnamic acids and ascorbate increased as plants aged. An ecotype from Acacia Bay had a higher phenolic content and antioxidant activities than one from Oamaru; these differences were maintained across generations as well as in calli from in vitro cultures. This indicates heritability and genetic fidelity of antioxidant potential. Chilling and salinity had variable effects on concentrations of phenolics and antioxidant activities in plants, and the combination of the two stressors was not synergistic. This indicates that these two stressors share signalling and response pathways. Stressor-induced increases in antioxidant activities of leaf extracts correlated with improved cellular antioxidant activities (CAA) inside HepG2 cells. Antioxidants were released from leaves following in vitro gastrointestinal digestion, which were then subsequently uptaken by Caco2 and HepG2 cells wherein they displayed CAAs. Thus, elevated levels of antioxidants in stressor-imposed plants provide potentially more antioxidant protection to live human cells. Caftaric, chlorogenic and chicoric acids accounted for 92% of the phenolic compounds in S. oleraceus leaves. Of these, only chlorogenic acid was inducible by stressors, both in intact plants and in calli. In young stressor-applied plants, chlorogenic acid was enhanced to the levels achievable with plant ageing. Boiling leaves prior to digestion did not diminish the caftaric and chlorogenic acid levels released through digestion, but chicoric acid levels were. Out of the nine phenolic compounds in leaves, only chicoric, chlorogenic and caftaric acids were released into the medium during in vitro gastrointestinal digestion. Digestion of leaves resulted in effective release of caftaric and chlorogenic acids from leaves but the levels of released chicoric acid were diminished by digestion. This study offers insights into the factors that influence the antioxidant potential of S. oleraceus L. in vivo, in vitro, during cooking and in vitro gastrointestinal digestion. These results provide the foundation for: (1) encouraging the consumption of its fresh shoots as an antioxidant rich food; (2) further improving its antioxidant activities through manipulation of agronomy, ecotype and breeding; (3) developing its cell cultures as a commercial platform for phyto-antioxidant production aimed at formulating dietary supplements or food additives in biopharmaceutical industry.</p>

Antioxidant Activities of Sonchus oleraceus L.

<p>Supernumerary free radicals and other reactive species can cause oxidative damage in animal cells, potentially leading to non-infectious diseases. Diets rich in low molecular weight antioxidants (LMWAs) may prevent or arrest the pathogenesis of these diseases. Leaves of Sonchus oleraceus L. may be an excellent dietary LMWA source for humans given their apparent strong antioxidant activities in vitro. However, different S. oleraceus plants vary in their antioxidant capacity. Nothing is known of possible environmental effects on antioxidant potential. Equally, the effects of cooking and gastrointestinal digestion are unknown. The goals of this research were: (i) to study the effects of plant age, locality, and abiotic stressors on antioxidant potential; (ii) to study the effects of cooking and in vitro gastrointestinal digestion on antioxidant activity and uptake in human cells; and (iii) to study extractable antioxidant activities of S. oleraceus cell suspension cultures in relation to abiotic stressors. Antioxidant activities and levels of total phenolics, hydroxycinnamic acids and ascorbate increased as plants aged. An ecotype from Acacia Bay had a higher phenolic content and antioxidant activities than one from Oamaru; these differences were maintained across generations as well as in calli from in vitro cultures. This indicates heritability and genetic fidelity of antioxidant potential. Chilling and salinity had variable effects on concentrations of phenolics and antioxidant activities in plants, and the combination of the two stressors was not synergistic. This indicates that these two stressors share signalling and response pathways. Stressor-induced increases in antioxidant activities of leaf extracts correlated with improved cellular antioxidant activities (CAA) inside HepG2 cells. Antioxidants were released from leaves following in vitro gastrointestinal digestion, which were then subsequently uptaken by Caco2 and HepG2 cells wherein they displayed CAAs. Thus, elevated levels of antioxidants in stressor-imposed plants provide potentially more antioxidant protection to live human cells. Caftaric, chlorogenic and chicoric acids accounted for 92% of the phenolic compounds in S. oleraceus leaves. Of these, only chlorogenic acid was inducible by stressors, both in intact plants and in calli. In young stressor-applied plants, chlorogenic acid was enhanced to the levels achievable with plant ageing. Boiling leaves prior to digestion did not diminish the caftaric and chlorogenic acid levels released through digestion, but chicoric acid levels were. Out of the nine phenolic compounds in leaves, only chicoric, chlorogenic and caftaric acids were released into the medium during in vitro gastrointestinal digestion. Digestion of leaves resulted in effective release of caftaric and chlorogenic acids from leaves but the levels of released chicoric acid were diminished by digestion. This study offers insights into the factors that influence the antioxidant potential of S. oleraceus L. in vivo, in vitro, during cooking and in vitro gastrointestinal digestion. These results provide the foundation for: (1) encouraging the consumption of its fresh shoots as an antioxidant rich food; (2) further improving its antioxidant activities through manipulation of agronomy, ecotype and breeding; (3) developing its cell cultures as a commercial platform for phyto-antioxidant production aimed at formulating dietary supplements or food additives in biopharmaceutical industry.</p>

Metabolic Profile and Mycoherbicidal Activity of Three Alternaria alternata Isolates for the Control of Convolvulus arvensis, Sonchus oleraceus, and Xanthium strumarium

Alternaria alternata isolates C1, S1, and X3 were isolated respectively from the weeds Convolvulus arvensis, Sonchus oleraceus, and Xanthium strumarium in Algiers during 2016 and identified by morphological and molecular analyses. The aim of this investigation was to chemically characterize the exometabolome of these fungi and to evaluate the myco-herbicidal potential of their culture filtrates, crude extracts, or fractions towards target weeds. Results revealed a great heterogeneity in the biochemical profiles of the exometabolome with the remarkable presence of two compounds: tenuazonic acid (TeA) and triprenyl phenol-7 (SMTP-7). To the best of our knowledge, SMTP-7—found in all the isolates—as well as 12-methoxycitromycin detected in the culture filtrate of isolate C1, have never been reported to be produced by A. alternata. Some fractions of isolates C1 and S1 showed symptoms (necrosis and chlorosis) on the detached leaves of C. arvensis and S. oleraceus, respectively with up to 100% phytotoxic effect at low concentration. In conclusion, biochemical characterization revealed great difference of C1, S1, and X3 exometabolomethat is likely to explain the difference in their phytotoxic activity. Some fractions (d1, e1, h1, i1, a2, and f2) of isolates C1 and S1 of A. alternata caused severe necrosis and chlorosis on the injured detached leaves of C. arvensis and S. oleraceus, respectively.

Effect of Soil Moisture Regimes on the Glyphosate Sensitivity and Morpho-Physiological Traits of Windmill Grass (Chloris truncata R.Br.), Common Sowthistle (Sonchus oleraceus L.), and Flaxleaf Fleabane [Conyza bonariensis (L.) Cronq.]

The glasshouse study was conducted with the objectives of (i) investigating the effect of soil moisture variations on the control efficiency of glyphosate on windmill grass (Chloris truncata R.Br.), common sowthistle (Sonchus oleraceus L.), and flaxleaf fleabane [Conyza bonariensis (L.) Cronq.], (ii) evaluating the tolerance of tested weed species under soil moisture variations, and (iii) determining the morphological and physiological characteristics of these species to partially explain herbicide tolerance under periods of reduced soil moisture availability (RSM). The species’ tolerance to glyphosate increased significantly under reduced soil moisture availability (p < 0.001). The lethal dose to cause herbicide injury or biomass reduction by 50% (LD50) and 80% (LD80) in relation to untreated control for water-stressed plants [i.e., moderate soil moisture availability (MSM) and RSM] was significantly higher than that of plants grown under high soil moisture availability (HSM). The tolerance factor (TF) for C. truncata, S. oleraceus, and C. bonariensis, in terms of biomass reduction under RSM, was 2.6, 2.4, and 2.6, respectively, as compared to HSM. The results showed that the glyphosate sensitivity, especially at the sub-lethal rates, of the three weed species under study decreased as soil moisture availability reduced (p < 0.01). Overall glyphosate efficacy, in relation to the recommended rate, was unaffected, except for C. truncata; the weed survived the highest tested glyphosate rate [750 g active ingredient (a.i.) ha−1] under RSM. There was significant interaction between weed species and soil moisture regimes for weed morpho-physiological traits (p < 0.001), with reduced soil moisture having a more influential impact on the growth of C. bonariensis and S. oleraceus compared to C. truncata. Changes in the leaf characteristics, such as increased leaf thickness, higher leaf chlorophyll content, reduced leaf area, and limited stomatal activity for all the tested weed species under MSM and RSM in relation to HSM, partially explain the tolerance of species to glyphosate at sublethal rates.

تأثير بعض المستخلصات النباتية والرواشح الفطرية على الفترة الزمنية لأنبات وقتل بذور المديد L. Convolvulus arvensis

اختلف التردد العددي لانبات وقتل بذور المديد L. Convolvulus arvensis في التركيزين 50 و 100 ملغم/مل للمستخلصات النباتية حيث شرع اول انبات للمعاملة السيطرة في اليوم الرابع بينما سجل اول انبات للمعاملة نبات الحندقوق Melilotus indicus في اليوم السادس , بالنسبة للتركيز 50 ملغم/مل وقد سجل اول حالة قتل في اليوم الحادي عشر بالنسبة للنوع Euphorbia peplus في حين تفوق المستخلص النباتي الجرجير Eruca vesicaria على جميع المعاملات في عملية القتل في جميع ايام الدراسة اما عند التركيز 100 ملغم/مل فقد سجل اول انبات لبذور المديد في اليوم السابع في معاملة مستخلص ام الحليب Sonchus oleraceus بينما سجلت اول حالة قتل في اليوم الرابع عشر بالنسبة لمستخلص فربيون Euphorbia peplus و مستخلص الكلغان Silybum marianum , كما اختلف التردد العددي لتأثير الرواشح الفطرية في انبات بذور المديد في التركيز 50 ملغم/مل . سجل اول شروع للانبات في اليوم الخامس في معاملة الراشح Penicillium resticulosum بينما سجلت اول حالة قتل في اليوم العاشر للراشح A. alternate اما في التركيز 100 ملغم/مل فقد سجلت اول حالة انبات في اليوم السادس للراشح الفطري Penicillium resticulosum كما سجلت اول حالة قتل للبذور في اليوم الحادي عشر للراشح Alternaria alternata.

A cytomorphological investigation of three species of the genus Sonchus L. (Asterales: Asteraceae) from Punjab, India

Three species of the genus Sonchus L. (Sonchus asper, S. oleraceus and S. wightianus) were collected from the Malwa region of Punjab during 2019 to 2020. These species were studied for cytomorphological variations. The species under investigation were identified based on their morphological descriptions. Sonchus asper (L.) Hill and Sonchus wightianus DC. possess the same number of chromosomes (2n=2x=18) whereas Sonchus oleraceus (L.) L. is tetraploid with 2n=4x=32 chromosomes. Chromosome number of S. wightianus (2n=2x=18) was worked out for the first time from the state of Punjab. Sonchus olereceus has larger pollens than S. asper and S. wightianus. This study will be useful for researchers, taxonomists and cytologists for accurate identification of these three species.

Effectiveness of glufosinate, dicamba, and clethodim on glyphosate-resistant and susceptible populations of five key weeds in Australian cotton systems

XtendFlexTM cotton with resistance to glyphosate, glufosinate and dicamba may become available in Australia. Resistance to these herbicides enables two additional modes of action to be applied in crop. The double knock strategy, typically glyphosate followed by paraquat, has been a successful tactic for control of glyphosate-resistant in fallow situations in Australia. Glufosinate is a contact herbicide, and may be useful as the second herbicide in a double knock for use in XtendFlexTM cotton crops. We tested the effectiveness of glufosinate applied at intervals of 1, 3, 7, and 10 d after initial applications of glyphosate, dicamba, clethodim and glyphosate mixtures with dicamba or clethodim on glyphosate-resistant and susceptible populations of Conyza bonariensis, Sonchus oleraceus, Chloris virgata, Chloris truncata and Echinochloa colona. Effective treatments for Conyza bonariensis with 100% control were dicamba and glyphosate+dicamba followed by glufosinate independent of the interval between applications. Sonchus oleraceus was effectively controlled in Experiment 1 by all treatments. However, in Experiment 2 effective treatments were dicamba and glyphosate+dicamba followed by glufosinate (99.3 – 100% control). Timing of the follow-up glufosinate did not affect the control achieved. Consistent control of Chloris virgata was achieved with glyphosate, clethodim or glyphosate+clethodim followed by glufosinate at 7 and 10 d intervals (99.7 – 100% control). Control of Chloris truncata was inconsistent. The best treatment for C. truncata was glyphosate+clethodim followed by glufosinate 10 d later (99.8 – 100% control). Echinochloa colona was effectively controlled with all treatments except for glyphosate on the glyphosate-resistant population. Additional in-crop use of glufosinate and dicamba should be beneficial for weed management in XtendFlexTM cotton crops, when utilising the double knock tactic with glufosinate. For effective herbicide resistance management, it is important that these herbicides be used in addition to, rather than substitution for, existing weed management tactics.

A, The Evaluating Allelopathic potential of aqueous extracts of Sow thistle (Sonchus oleraceus L.) on emergence and seedling growth of Red rice (Oryza punctata)

ABSTRACT Chemical weed control method caused environmental hazards and residual effects in crops and soil. Alternate approach to control weeds is getting attentions in sustainable production system. Use of aqueous extracts of weed is getting scientific attention as eco-friendly alternative to chemical herbicides especially under scenario of fast increasing herbicide resistance in weeds. To investigate the herbicidal potential of aqueous extract of winter weed of Sonchus oleraceus L. (Sow thistle) on summer weed Oryza punctate L. (red rice) seed emergence and initial seedling growth, a study was planned. In this study, seeds of O. punctate were incubated in seven concentrations (0, 0.25, 0.50, 1, 2, 4, and 8%) of different parts i.e. leaves, stem, flower of S. oleraceus. All the tested concentrations of various plant parts of S. oleraceus significantly inhibited the mean emergence time, emergence index, emergence percentage (%), time taken to 50% emergence as well as growth of O. punctata weed. However, maximum mean emergence time (5.26 days), minimum germination index (1.69), germination percentage (40%), root length (2.04 cm), shoot length (5.71 cm), fresh weight (59 g), dry weight (4.06 g) was noted at 8% concentration with fruit aqueous extract of S. oleraceus. Seed of O. punctata take maximum time to complete 50% emergence (3.33 days) under control where we applied distilled water. Results suggested fruit extract of S. oleraceus at 8% concentration can be used potential bio-herbicide for the control of O. punctate. Key words: Chemical, eco-friendly, herbicidal potential, fruit extract