scholarly journals Anatomo-Histological Evaluation of Male Reproductive System of the Juvenile Caiman crocodilus yacare

2021 ◽  
Vol 39 (6) ◽  
pp. 1688-1693
Author(s):  
Cristiane Naoko Takamine ◽  
Hianka Jasmyne Costa de Carvalho ◽  
Michelle Silva Araújo ◽  
Rafaela Rodrigues Ribeiro ◽  
Maria Angélica Miglino ◽  
...  
Keyword(s):  
Reproductive System ◽  
Male Reproductive System ◽  
Histological Evaluation ◽  
Caiman Crocodilus

scholarly journals Cystatin C Is Highly Expressed in the Human Male Reproductive System

2004 ◽  
Vol 25 (4) ◽  
pp. 564-572 ◽  
Author(s):  
Thomas Jiborn ◽  
Magnus Abrahamson ◽  
Hanna Wallin ◽  
Johan Malm ◽  
Åke Lundwall ◽  
...  
Keyword(s):  
Cystatin C ◽  
Reproductive System ◽  
Male Reproductive System ◽  
Human Male

scholarly journals Presence and Characterization of Cell-Free Seminal RNA in Healthy Individuals: Implications for Noninvasive Disease Diagnosis and Gene Expression Studies of the Male Reproductive System

2009 ◽  
Vol 55 (11) ◽  
pp. 1967-1976 ◽  
Author(s):  
Shiyun Huang ◽  
Honggang Li ◽  
Xiaofang Ding ◽  
Chengliang Xiong
Keyword(s):  
Reproductive System ◽  
Time Course ◽  
Disease Diagnosis ◽  
Male Reproductive System ◽  
Healthy Individuals ◽  
Microcapillary Electrophoresis ◽  
Expression Studies ◽  
Noninvasive Biomarker ◽  
Gene Expression Studies ◽  
Triton X

Abstract Background: We recently detected cell-free seminal RNA (cfsRNA) and set out to study its concentration, integrity, stability in healthy individuals, and mechanisms for its protection from ribonucleases. Methods: We quantified cfsRNA by reverse-transcription quantitative real-time PCR (RT-qPCR) targeting of the 5′ region of the ACTB (actin, beta) transcript. cfsRNA integrity was analyzed by microcapillary electrophoresis and by amplification of full-length ACTB and DDX4 [DEAD (Asp-Glu-Ala-Asp) box polypeptide 4] transcripts, including measurement of the relative amounts of different regions of ACTB and DDX4 transcripts. Stability of cfsRNA was measured by time-course analysis of different regions of ACTB and DDX4 transcripts. To investigate whether cfsRNA was protected in complexed forms, we processed seminal plasma in 2 ways: filtration through pores of different sizes and Triton X-100 treatment before RNA recovery. Results: cfsRNA concentrations varied from 0.87–3.64 mg/L [mean (SD), 1.75 mg/L (0.92 mg/L)]. Most cfsRNA was present in partially degraded forms, with smaller amounts of middle and 3′ amplicons compared with 5′ amplicons. Although the 3′ region of the DDX4 transcript was degraded completely by 90 min, the 5′ regions of ACTB and DDX4 transcripts were stable up to 24 h. Filtration through 0.22-μm pores reduced ACTB and DDX4 mRNA concentrations by 72% and 61%, respectively. Nearly all seminal ACTB and DDX4 mRNA disappeared after Triton X-100 treatment. Conclusions: Although cfsRNA was partially degraded, it represented diverse transcript species and was abundant, fairly stable, and associated with particles in healthy individuals. cfsRNA may represent a potential noninvasive biomarker of the male reproductive system and of germline epigenetics.

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