scholarly journals Genetic characterization of Japanese plum cultivars (Prunus salicina) using SSR and ISSR molecular markers

2012 ◽  
Vol 39 (3) ◽  
pp. 533-543 ◽  
Author(s):  
Basilio Carrasco ◽  
Carole Díaz ◽  
Mario Moya ◽  
Marlene Gebauer ◽  
Rolando García-González
Keyword(s):  
Molecular Markers ◽  
Genetic Characterization ◽  
Japanese Plum ◽  
Prunus Salicina ◽  
Issr Molecular Markers

scholarly journals Characterization of Genetic Diversity in Accessions of Prunus salicina Lindl: Keeping Fruit Flesh Color Ideotype While Adapting to WaterStressed Environments

Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 487 ◽  
Author(s):  
Acuña ◽  
Rivas ◽  
Brambilla ◽  
Cerrillo ◽  
Frusso ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Candidate Genes ◽  
Stress Resistance ◽  
Prunus Persica ◽  
Agronomic Traits ◽  
Phenotypic Traits ◽  
Flesh Color ◽  
Japanese Plum ◽  
Prunus Salicina

The genetic diversity of 14 Japanese plum (Prunus salicina Lindl) landraces adapted to an ecosystem of alternating flooding and dry conditions was characterized using neutral simple sequence repeat (SSR) markers. Twelve SSRs located in six chromosomes of the Prunus persica reference genome resulted to be polymorphic, thus allowing identification of all the evaluated landraces. Differentiation between individuals was moderate to high (average shared allele distance (DAS) = 0.64), whereas the genetic diversity was high (average indices polymorphism information content (PIC) = 0.62, observed heterozygosity (Ho) = 0.51, unbiased expected heterozygosity (uHe) = 0.70). Clustering and genetic structure approaches grouped all individuals into two major groups that correlated with flesh color. This finding suggests that the intuitive breeding practices of growers tended to select plum trees according to specific phenotypic traits. These neutral markers were adequate for population genetic studies and cultivar identification. Furthermore, we assessed the SSR flanking genome regions (25 kb) in silico to search for candidate genes related to stress resistance or associated with other agronomic traits of interest. Interestingly, at least 26 of the 118 detected genes seem to be related to fruit quality, plant development, and stress resistance. This study suggests that the molecular characterization of specific landraces of Japanese plum that have been adapted to extreme agroecosystems is a useful approach to localize candidate genes which are potentially interesting for breeding.

Genetic characterization of apospory in tetraploid Paspalum notatum based on the identification of linked molecular markers

2003 ◽  
Vol 12 (4) ◽  
pp. 319-327 ◽  
Author(s):  
Eric J. Martínez ◽  
H. Esteban Hopp ◽  
Juliana Stein ◽  
Juan P.A. Ortiz ◽  
Camilo L. Quarin
Keyword(s):  
Molecular Markers ◽  
Genetic Characterization ◽  
Paspalum Notatum

scholarly journals Identification and Genetic Diversity Analysis of Edible and Medicinal Malva Species Using Flow Cytometry and ISSR Molecular Markers

Agronomy ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 650
Author(s):  
Iwona Jedrzejczyk ◽  
Monika Rewers
Keyword(s):  
Flow Cytometry ◽  
Molecular Markers ◽  
Genome Size ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Size Estimation ◽  
Flow Cytometric ◽  
First Choice ◽  
Issr Molecular Markers

The Malva genus contains species that reveal therapeutic properties and are mostly important in medicine and the functional food industry. Its breeding, cultivation, and utilization are based on proper germplasm/plant identification, which is difficult using morphological features. For this reason, we applied flow cytometry and inter simple sequence repeat polymerase chain reaction (ISSR-PCR) for fast and accurate species identification. Genome size estimation by flow cytometry was proposed as the first-choice method for quick accession screening. Out of the 12 tested accessions, it was possible to identify six genotypes based on genome size estimation, whereas all species and varieties were identified using ISSR markers. Flow cytometric analyses revealed that Malva species possessed very small (1.45–2.77 pg/2C), small (2.81–3.80 pg/2C), and intermediate (11.06 pg/2C) genomes, but the majority of accessions possessed very small genomes. Additionally, this is the first report on genome size assessment for eight of the accessions. The relationships between the investigated accessions showed the presence of two clusters representing malvoid and lavateroid group of species. Flow cytometry and ISSR molecular markers can be effectively used in the identification and genetic characterization of Malva species.

scholarly journals Genetic characterization of fig tree mutants with molecular markers

2012 ◽  
Vol 11 (3) ◽  
pp. 1990-1996 ◽  
Author(s):  
M.G.F. Rodrigues ◽  
A.B.G. Martins ◽  
J.A. Desidério ◽  
B.W. Bertoni ◽  
M.C. Alves
Keyword(s):  
Molecular Markers ◽  
Genetic Characterization ◽  
Fig Tree

scholarly journals GENETIC CHARACTERIZATION OF GALOBA DURIAN (AMONUM SPP.) IN AMBON ISLAND BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)

2018 ◽  
Vol 3 (1) ◽  
pp. 27-33
Author(s):  
Fuadiska Salamena ◽  
Cecilia Anna Seumahu
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Genetic Characterization ◽  
Kidney Diseases ◽  
Random Amplified Polymorphic Dna ◽  
Quantitative Result ◽  
Dna Amplification ◽  
Polymorphic Band ◽  
Qualitative And Quantitative

Galoba durian is one of the endemic floras from Maluku. Galoba durian is a species belonging to the Amomum genera Zingiberaceae family. It is also used as a medicinal plant for waist and kidney diseases. Based on the color of the fruit, galoba durian is divided into two nmely red galoba durian and green galoba durian. Distribution of this plant in Ambon can found in a few places such as highland and coastal area. Different locations influence phenotypic of plants, but may not show different genetic characteristic. Genetic diversity can detected by molecular markers. Genetic characterization from galoba durian using RAPD markers has not been done before. This study aimed to analyze genetic diversity from galoba durian using molecular markers RAPD. Samples of plants are used red galoba durian from Hatu and green galoba durian from Hatalae. The result of the first study, characterization of the morphology of the galoba durian, showed that both galoba have almost similar characteristics. Further DNA was tested by qualitative and quantitative. Result shows good qualitative and quantitative of DNA genomic. The second study was amplification by PCR-RAPD. DNA amplifications were performed using 3 primers out of 9 screened random primers. The primers selection was based on hight polymorphism. DNA amplification has 36 bands which were 100% polymorphic. The size of each bands from visualization of agarose was determined by linear regression. Number of band amplified was range from 120 to 1612 bp. Polymorphic band of RAPD showed the highest  genetic diversity. It can be concluded that the two plants of galoba durian are different species

scholarly journals Genetic characterization of Aberdeen Angus cattle using molecular markers

2003 ◽  
Vol 26 (2) ◽  
pp. 133-137 ◽  
Author(s):  
Luciana Pimentel de Mello Klocker Vasconcellos ◽  
Daniella Tambasco-Talhari ◽  
Andréa Pozzi Pereira ◽  
Luiz Lehmann Coutinho ◽  
Luciana Correia de Almeida Regitano
Keyword(s):  
Molecular Markers ◽  
Genetic Characterization ◽  
Angus Cattle

scholarly journals Morphological and genetic characterization of vine grape cultivars of Herzegovina

2017 ◽  
Vol 3 (2) ◽  
pp. 1-9
Author(s):  
Zrinka Knezović ◽  
Ana Mandić ◽  
Nikica Perić ◽  
Jure Beljo ◽  
Maja Žulj Mihaljević
Keyword(s):  
Molecular Markers ◽  
Genetic Characterization ◽  
Morphological Characterization ◽  
Similarity Coefficient ◽  
Value Range ◽  
Grape Cultivars

Abstract In Herzegovina, different genotypes of grapevine are called the same, and the origin of these cultivars is mainly unexplored. Two groups of autochthonous cultivars are grown. The first group consists of more widespread Žilavka and Blatina, and accompanying Bena, Krkošija, Dobrogostina and Trnjak, and the second one of less common cultivars that should be preserved from extinction. Considering the small number of studies on the comparison of different methods used for analyses of similarities and relatedness of grapevine germplasm, it was interesting to assess the use of morphological characterization with molecular markers in identification of cultivars in Herzegovina. The results indicate that a similarity coefficient had a broad value range for both methods. Both methods successfully in different ways differentiated all analysed varieties, and for complete and comprehensive identification of grapevine varieties should be use.

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