scholarly journals Application of innovative hemocytometric parameters and algorithms for improvement of microcytic anemia discrimination

2015 ◽  
Vol 7 (2) ◽  
Author(s):  
Margreet Schoorl ◽  
Marianne Schoorl ◽  
Johannes Van Pelt ◽  
Piet C.M. Bartels
Keyword(s):  
Iron Deficiency ◽  
Blood Cell ◽  
Iron Deficiency Anemia ◽  
Red Blood Cell ◽  
Hematological Parameters ◽  
Deficiency Anemia ◽  
Zinc Protoporphyrin ◽  
Distribution Width ◽  
New Algorithms ◽  
Rbc Count

Hemocytometric parameters like red blood cell (RBC) count, mean red blood cell volume (MCV), reticulocyte count, red blood cell distribution width (RDW-SD) and zinc protoporphyrin (ZPP) are frequently established for discrimination between iron-deficiency anemia and thalassemia in subjects with microcytic erythropoiesis. However, no single marker or combination of tests is optimal for discrimination between iron-deficiency anemia and thalassemia. This is the reason why many algorithms have been introduced. However, application of conventional algorithms, only resulted in appropriate classification of 30-40% of subjects. In this mini-review the efficacy of innovative hematological parameters for detection of alterations in RBCs has been considered. It refers to parameters concerning hemoglobinization of RBCs and reticulocytes and the percentages microcytic and hypochromic RBCs, for discrimination between subjects with iron-deficiency anemia (IDA) or thalassemia as well as a combination of both. A new discriminating tool including the above mentioned parameters was developed, based on two precondition steps and discriminating algorithms. The percentage microcytic RBCs is considered in the first pre-condition step. MCV, RDW-SD and RBC count are applied in the second precondition step. Subsequently, new algorithms, including conventional as well as innovative hematological parameters, were assessed for subgroups with microcytic erythropoiesis. The new algorithms for IDA discrimination yielded results for sensitivity of 79%, specificity of 97%, positive and negative predictive values of 74% and 98% respectively. The algorithms for beta-thalassemia discrimination revealed similar results (74%, 98%, 75% and 99% respectively). We advocate that innovative algorithms, including parameters reflecting hemoglobinization of RBCs and reticulocytes, are integrated in an easily accessible software program linked to the hematology equipment to improve the discrimination between IDA and thalassemia.

scholarly journals Red blood cell distribution width and iron deficiency anemia among pregnant Sudanese women

2012 ◽  
Vol 7 (1) ◽  
pp. 168 ◽  
Author(s):  
Esam G Abdelrahman ◽  
Gasim I Gasim ◽  
Imad R Musa ◽  
Leana M Elbashir ◽  
Ishag Adam
Keyword(s):  
Iron Deficiency ◽  
Blood Cell ◽  
Iron Deficiency Anemia ◽  
Red Blood Cell ◽  
Deficiency Anemia ◽  
Cell Distribution ◽  
Distribution Width ◽  
Red Blood Cell Distribution

scholarly journals IRON DEFICIENCY ANEMIA

2018 ◽  
Vol 25 (05) ◽  
pp. 659-663
Author(s):  
Nathumal Maheshwari ◽  
Omperkash Khemani ◽  
Bilawal Hingorjo ◽  
Mehmood Shaikh ◽  
Siri Chand ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Blood Cell ◽  
Iron Deficiency Anemia ◽  
Red Blood Cell ◽  
Venous Blood ◽  
Deficiency Anemia ◽  
Cell Distribution ◽  
Cross Sectional ◽  
Distribution Width ◽  
Red Blood Cell Distribution

Objectives: To evaluate the predictive value of Red blood cell distribution width(RDW) for the iron deficiency anemia in children. Study Design: Cross sectional study. Placeand Duration: Department of Paediatrics, Layari General Hospital Shaheed Muhtrama BenazirBhutto Medical College from December 2015 to March 2016. Methodology: A sample of 100children (53 male and 47 female) was selected through non- probability (purposive sampling). 5ml venous blood was collected, 3 ml was shifted to EDTA containing vacutainers and remainingwas processed and centrifuged to separate sera. Complete blood counts and iron profile wereperformed. Data of cases and controls was analyzed on Statistix 10.0 software (USA) (P ≤0.05).Results: Severe iron deficiency and iron deficiency anemia were noted. Serum Iron, TIBC andFerritin were noted as 63.49±32.94 and 76.06±40.38 μg/dl, 468.7±142.2 and 445.5±135.2 μg/dl, & 36.2±14.12 and 43.2±13.5 ng/dl respectively. RDW proved a sensitivity and specificity of78% and 56% respectively (0.001). Conclusion: We observed severe iron deficiency anemia inchildren and Red blood cell distribution width showed high sensivity in predicting iron deficiencyanemia.

Distinguishing iron deficiency anemia from thalassemia by the red blood cell lifespan with a simple CO breath test: a pilot study

2019 ◽  
Vol 13 (2) ◽  
pp. 026007
Author(s):  
Mei-qing Lei ◽  
Ling-feng Sun ◽  
Xian-sheng Luo ◽  
Xiao-yang Yang ◽  
Feng Yu ◽  
...  
Keyword(s):  
Pilot Study ◽  
Iron Deficiency ◽  
Blood Cell ◽  
Iron Deficiency Anemia ◽  
Red Blood Cell ◽  
Breath Test ◽  
Deficiency Anemia ◽  
Cell Lifespan

scholarly journals Inherited microcytic anemias

Hematology ◽  
2020 ◽  
Vol 2020 (1) ◽  
pp. 465-470
Author(s):  
Maria Domenica Cappellini ◽  
Roberta Russo ◽  
Immacolata Andolfo ◽  
Achille Iolascon
Keyword(s):  
Iron Deficiency ◽  
Blood Cell ◽  
Iron Metabolism ◽  
Red Blood Cell ◽  
Iron Status ◽  
Transferrin Saturation ◽  
Microcytic Anemia ◽  
Deficiency Anemia ◽  
Mean Corpuscular Hemoglobin ◽  
Distribution Width

Abstract Inherited microcytic anemias can be broadly classified into 3 subgroups: (1) defects in globin chains (hemoglobinopathies or thalassemias), (2) defects in heme synthesis, and (3) defects in iron availability or iron acquisition by the erythroid precursors. These conditions are characterized by a decreased availability of hemoglobin (Hb) components (globins, iron, and heme) that in turn causes a reduced Hb content in red cell precursors with subsequent delayed erythroid differentiation. Iron metabolism alterations remain central to the diagnosis of microcytic anemia, and, in general, the iron status has to be evaluated in cases of microcytosis. Besides the very common microcytic anemia due to acquired iron deficiency, a range of hereditary abnormalities that result in actual or functional iron deficiency are now being recognized. Atransferrinemia, DMT1 deficiency, ferroportin disease, and iron-refractory iron deficiency anemia are hereditary disorders due to iron metabolism abnormalities, some of which are associated with iron overload. Because causes of microcytosis other than iron deficiency should be considered, it is important to evaluate several other red blood cell and iron parameters in patients with a reduced mean corpuscular volume (MCV), including mean corpuscular hemoglobin, red blood cell distribution width, reticulocyte hemoglobin content, serum iron and serum ferritin levels, total iron-binding capacity, transferrin saturation, hemoglobin electrophoresis, and sometimes reticulocyte count. From the epidemiological perspective, hemoglobinopathies/thalassemias are the most common forms of hereditary microcytic anemia, ranging from inconsequential changes in MCV to severe anemia syndromes.

Usefulness of red blood cell flags in diagnosing and differentiating thalassemia trait from iron-deficiency anemia

Hematology ◽  
2008 ◽  
Vol 13 (4) ◽  
pp. 253-256 ◽  
Author(s):  
J. Eivazi-Ziaei ◽  
S. Dastgiri ◽  
S. Pourebrahim ◽  
R. Soltanpour
Keyword(s):  
Iron Deficiency ◽  
Blood Cell ◽  
Iron Deficiency Anemia ◽  
Red Blood Cell ◽  
Deficiency Anemia ◽  
Thalassemia Trait

scholarly journals THE STATE OF THE RED BLOOD CELL MEMBRANE IN IRON DEFICIENCY ANAEMIA IN WOMEN OF REPRODUCTIVE AGE

2019 ◽  
Vol 16 (32) ◽  
pp. 471-477
Author(s):  
Leila I. ARYSTAN ◽  
Gulmira M. MULDAEVA ◽  
Leila S. HAYDARGALIEVA ◽  
Damira K. PAKHOMOVA ◽  
Yernar B. ISKAKOV
Keyword(s):  
Iron Deficiency ◽  
Cell Membrane ◽  
Blood Cell ◽  
Iron Deficiency Anemia ◽  
Red Blood Cell ◽  
The State ◽  
Reproductive Age ◽  
Deficiency Anemia ◽  
Women Of Reproductive Age ◽  
Red Blood Cell Membrane

The problem of iron deficiency is urgent in many countries, regardless of the socio-economic standards of living. Among biomedical problems, the study of biochemical blood parameters is of paramount importance. A great interest in various aspects of this issue is not accidental, since the transport function of the blood largely depends on it and, consequently, the efficient delivery of oxygen, glucose, amino acids, fats, vitamins, mineral salts, hormones, mediators, antibodies and metabolites to the micro-zones in the tissues. The purpose of this article is to study the hematological status and the state of the red blood cell membrane in women of reproductive age suffering from iron deficiency anemia (IDA). Iron deficiency anemia (IDA) is a combination of clinical and hematological symptoms characterized by impaired hemoglobin formation due to iron deficiency in serum and bone marrow, as well as the development of trophic disorders in the organs and tissues. During the study, a complete blood count was performed using an enzyme-linked immunosorbent assay, using the kits produced by JSC Vector-Best, and the Kamyshnikov method. Based on the results of the study of the erythrocyte sorption capacity (ESC) in women of reproductive age with IDA, it was established that this parameter is 1.2 times higher than that in the control group. The development of anemia is associated with significant changes in the osmotic stability of the erythrocytes, which results in an increase in the proportion of both unstable and high-resistance erythrocytes. The scientific novelty of the article is that the authors established the key parameters for iron deficiency anemia detection.

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