scholarly journals Effects of vitamin E and phosphatidylcholine on qualitative and quantitative parameters of rainbow trout (Oncorhynchus mykiss) milt

2007 ◽  
Vol 6 (4) ◽  
pp. 339-350 ◽  
Author(s):  
Simona Rainis ◽  
Laura Gasco ◽  
Rodolfo Ballestrazzi ◽  
Ivo Zoccarato
Keyword(s):  
Rainbow Trout ◽  
Vitamin E ◽  
Oncorhynchus Mykiss ◽  
Qualitative And Quantitative ◽  
Quantitative Parameters ◽  
Rainbow Trout Oncorhynchus Mykiss

Effect of vitamin E on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo

2004 ◽  
Vol 52 (4) ◽  
pp. 413-422 ◽  
Author(s):  
Ş. Aras-Hisar ◽  
O. Hisar ◽  
Ş. Beydemir ◽  
I. Gülçin ◽  
T. Yanik
Keyword(s):  
Enzyme Activity ◽  
Rainbow Trout ◽  
Vitamin E ◽  
Carbonic Anhydrase ◽  
Oncorhynchus Mykiss ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Blood Erythrocyte ◽  
The Difference

Considering that the excessive usage of vitamin E causes hypervitaminosis and thus reduces blood erythrocyte concentrations, therefore it is worth studying how its pharmacological dosage affects the activity of carbonic anhydrase (CA) enzyme found in erythrocytes of rainbow trout (Oncorhynchus mykiss) in vitro and in vivo. Vitamin E inhibited CA enzyme and the IC50 value of the vitamin was 0.039 mM in vitro. Similarly, it was seen that vitamin E inhibited CA enzyme activity after the first hour following vitamin E injections in vivo. The activities of CA in groups of trout given vitamin E injection were measured at 1, 3 and 5 h and the corresponding activities were found to be 772.7 ± 290.5 (P < 0.05), 1286.4 ± 378.2 and 1005.7 ± 436.1 enzyme units (EU) g Hb-1. The difference over the control was significant (P < 0.05) in the first hour and insignificant at 3 and 5 h (P ? 0.05). The activity of CA in the control, which did not contain vitamin E, was determined as 1597.7 ± 429.0 EU g Hb-1.

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