Analysis and Identification of Astaxanthin and its Carotenoid Precursors from Xanthophyllomyces dendrorhous by High-Performance Liquid Chromatography

2010 ◽  
Vol 65 (7-8) ◽  
pp. 489-494 ◽  
Author(s):  
Mingbo Lu ◽  
Yang’e Zhang ◽  
Chunfang Zhao ◽  
Pengpeng Zhou ◽  
Longjiang Yu
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Absorption Spectra ◽  
Mobile Phase ◽  
High Performance ◽  
Biosynthetic Pathway ◽  
Hplc Method ◽  
Xanthophyllomyces Dendrorhous ◽  
Hplc Chromatogram ◽  
Carotenoid Pathway

This study presents an HPLC method for simultaneous analysis of astaxanthin and its carotenoid precursors from Xanthophyllomyces dendrorhous. The HPLC method is accomplished by employing a C18 column and the mobile phase methanol/water/acetonitrile/ dichloromethane (70:4:13:13, v/v/v/v). Astaxanthin is quantified by detection at 480 nm. The carotenoid precursors are identified by LC-APCI-MS and UV-vis absorption spectra. Peaks showed in the HPLC chromatogram are identified as carotenoids in the monocyclic biosynthetic pathway or their derivatives. In the monocyclic carotenoid pathway, 3,3’-dihydroxy- β,ψ-carotene-4,4’-dione (DCD) is produced through γ-carotene and torulene.

scholarly journals Phenobarbital Analysis in Biological Matrix (Blood) by High Performance Liquid Chromatography (HPLC)

2013 ◽  
Vol 20 ◽  
pp. 31-40
Author(s):  
Ouakouak Hamza ◽  
Ben Mohamed Moktar ◽  
Ben Chohra Mostafa ◽  
Abdelhamid Zeghdaoui
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Ammonium Acetate ◽  
Analytical Techniques ◽  
Hplc Method ◽  
Diode Array ◽  
Blood Components ◽  
Ammonium Acetate Buffer

In this work, we have tried to contribute to the analysis of phenobarbital in the blood. To do this, we used different analytical techniques such as liquid chromatography (HPLC). The results obtained in the course of our work, we can conclude that: The HPLC method was separate phenobarbital endogenous blood components, and optimizing the conditions of chromatographic separation as the composition of the mobile phase consisting of 20% acetonitrile + 20% methanol + 60% ammonium acetate buffer. The extraction with diethyl ether to pH = 4; The chromatographic column, microbondapack ZORBAX C18. A system of diode-array UV detection at 254 nm.

Quantitation of Transdermal Tadalafil in Human Skin by Reversed-Phase High-Performance Liquid Chromatography

2012 ◽  
Vol 95 (4) ◽  
pp. 1064-1068 ◽  
Author(s):  
Mohammed H Mehanna ◽  
Abdel M Motawaa ◽  
Magda W Samaha
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Skin ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Hplc Method ◽  
Drug Deposition ◽  
Transdermal Permeation

Abstract A reliable and sensitive HPLC method was developed for the quantitation of tadalafil transdermal permeation through human skin. An RP column with UV detection at 290 nm was used for chromatographic separation at ambient temperature. The mobile phase was acetonitrile–water containing 20 mM pH 7 phosphate buffer (35/65, v/v) with a flow rate of 1.0 mL/min. The LOQ achieved was 1 ng/mL, and the calibration curve showed good linearity over the concentration range of 5–2000 ng/mL for tadalafil, with a determination coefficient (R2) of 0.998. The RSD values of intraday and interday analyses were all within 7%. Parameters of validation proved the precision of the method; this validated method was applied for the determination of tadalafil in transdermal permeation and drug deposition in human skin studies.

Analysis of Bifenthrin and its Enantiomer Using High Performance Liquid Chromatography

2014 ◽  
Vol 675-677 ◽  
pp. 275-279 ◽  
Author(s):  
Su Su Fan ◽  
Jian Shi ◽  
Ling Zhou ◽  
Yu Wen Hang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Chiral Stationary Phase ◽  
Ammonium Acetate ◽  
Hplc Method ◽  
Column Temperature ◽  
Polysaccharide Derivatives ◽  
Separation Results

Using the high performance liquid chromatography (HPLC) method, bifenthrin isomers can be split at a polysaccharide derivatives chiral stationary phase column, and two well distinguished peaks of bifenthrin isomers are obtained. The effects of mobile phase ratios, temperatures, and detection wavelengths on the separation results are discussed. The optimal chromatographic conditions are as follows: the mobile phase ratio is methanol: ammonium acetate salts = 80:20, the column temperature is 35°C, and the wavelength is set as 220 nm. Under the optimal conditions, the resolution of bifenthrin enantiomer can be as large as 3.0.

scholarly journals Determination of Ketotifen Fumarate in Syrup Dosage Form by High Performance Liquid Chromatography

2020 ◽  
pp. 63-72
Author(s):  
Mohammed Ali Salih ◽  
Dlivan Fattah Aziz ◽  
Salar Ibrahim Ali
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Hplc Method ◽  
Column Temperature ◽  
Ketotifen Fumarate ◽  
Suggested Technique ◽  
Elution Method

The goal of the current study was to establish and authenticate an isocratic reverse-stage High-Performance Liquid Chromatography (HPLC) method for quantifying ketotifen fumarate (KF) in pharmaceutical liquid dosage compositions. Easy, quick, accurate, exact, and accurate reverse-stage high-performance liquid chromatography was advanced for the simultaneous assessment of ketotifen fumarate in the liquid syrup dosage type. The HPLC system using isocratic elution method with reverse-phase Inertsil ODS-(250 mm × 4.6 mm, 3 μm) column was detected by ultraviolet absorbance at 297 nm with no interference from widely using excipients, the mobile phase (A) is a mixture of triethylamine and water (175 μl in 500 ml of water), and the mobile phase (B) is a mixture of triethylamine and methanol (175 μl in 500 ml of methanol) at a flow rate of 1.5 mL/min (mobile phase A 40 %:mobile phase B 60%) at column temperature using 40 ° C, the retention time for ketotifen fumarate was 6.4±0.5 min. The concentration curves were linear in the range of 10.0 to 35.0 μg / ml (R2 = 0.9999). The developed method was tested for the specificity, precision, linearity, precision, reliability, robustness, and consistency of the solution. The regeneration of ketotifen fumarate in formulations was found to be 99.75 %, 99.91 %, and 100.05 % respectively. The percent RSD for percent recovery was found to be 0.21 and 0.17 and 0.10 for ketotifen fumarate. In the conclusion, the suggested technique was successfully used for the quantitative determination of ketotifen fumarate in formulations.

scholarly journals Reverse-phase High-Performance Liquid Chromatography Method Development and Validation for Estimation of Glimepiride in Bulk and Tablet Dosage Form

2020 ◽  
Vol 11 (02) ◽  
pp. 296-302
Author(s):  
Aseem Kumar ◽  
Anil Kumar Sharma ◽  
Rohit Dutt
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Flow Rate ◽  
Retention Time ◽  
Mobile Phase ◽  
High Performance ◽  
Hplc Method ◽  
Reverse Phase ◽  
Rp Hplc ◽  
Development And Validation

The present work demonstrates a simple, rapid, precise, specific, and sensitive reverse-phase high-performance liquid chromatography (RP-HPLC) method for analyzing glimepiride in pure and tablet forms. The present method was developed using a C18 column 150 × 4.6 mm, with 5 μm, and packing L1 maintained at a temperature of 30°C. The mobile phase was prepared by dissolving 0.5 gram of monobasic sodium phosphate in 500 mL of distilled water, pH of the solution adjusted to 2.1 to 2.7 with 10% phosphoric acid, and added 500 mL of acetonitrile. The mobile phase was pumped in the highperformance liquid chromatography (HPLC) system at a flow rate of 1 mL/min, and separation was carried out at 228 nm, using an ultraviolet (UV) detector. The chromatographic separation was achieved with peak retention time (RT) at about 9.30 minutes, and the method was found to be linear over a concentration range of 40 to 140 μg/mL. The specificity of the method represented no interference of the excipients during the analysis, and stability testing after 24 hours also showed that the method is suitable and specific. The accuracy was between 99.93 to 99.96%, with limit of detection (LOD) and limit of quantitation (LOQ) being 0.354 μg/mL, 1.18 μg/mL, respectively. Satisfactory results were found for precision and robustness parameters during the development and validation stage for the analytical method. The proposed method was also adopted for the analysis of glimepiride tablets to improve the overall quality control. Using this method, symmetric peak shape was obtained with reasonable retention time. The retention time of glimepiride for six repetitions is 9.3 ± 0.1 minutes; the run time is 21 minutes. The proposed RP-HPLC method is a modification of the United States Pharmacopeia (USP) method, and it was found to be valid for glimepiride within concentration ranges 40 to 140 μg/mL, using C18 analytical columns, and isocratic elution with UV detection, and at 1 mL/min of flow rate.

Establishment and Evaluation of Determination of Cinnamaldehyde in Baoyuanqingxue Granules by HPLC

2014 ◽  
Vol 998-999 ◽  
pp. 372-377 ◽  
Author(s):  
Qiang Wu ◽  
Chang Hong Wang ◽  
Pu Wang ◽  
Xiang Rong Liu
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Extraction Method ◽  
Mobile Phase ◽  
High Performance ◽  
Hplc Method ◽  
Column Temperature ◽  
Average Recovery

To examine the extraction method and chromatographic conditions that affect the determination of cinnamaldehyde in Baoyuanqingxue granules and make clinical evaluation about the determination of cinnamaldehyde.Ultrasonic methanol extraction was used before the detemination of cinnamaldehyde in Baoyuanqingxue granules. High Performance Liquid chromatography (HPLC) method was applied to detect samples. The SB-C18 column (Agilent, ZORBAX, 4.6×150mm, 5μm) was adopted, the mobile phase was acetonitrile-water (35:65) at the flow rate of 1.00mL•min-1 with DAD detection wavelength at 290nm, the volume of injection was 20μL and the column temperature was 30°C. The resolution between cinnamaldehyde and other peaks was good. The calibration curve was linear in the range of 0.5035~50.35μg•mL-1(r=0.99976). The average recovery (n=6) of cinnamaldehyde was 99.2% with RSD of 0.5%. The HPLC-DAD method to detect the content of cinnamaldehyde in Baoyuanqingxue granules is simple and accurate. It can be used for quality control of cinnamaldehyde in Baoyuanqingxue granules.

Modified QuEChERS as a novel sample preparation method for analysis of N-nitrosodiethanolamine in shampoo by high performance liquid chromatography

2017 ◽  
Vol 9 (35) ◽  
pp. 5165-5173 ◽  
Author(s):  
Ghazaleh Abedi ◽  
Zahra Talebpour
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Preparation Method ◽  
Hplc Method ◽  
Sample Preparation Method ◽  
Modified Quechers ◽  
Rp Hplc ◽  
Novel Method

This study sought to develop a novel method for the trace analysis ofN-nitrosodiethanolamine (NDELA) in shampoos inspired by a Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) extraction, followed by an RP-HPLC method with a water-rich mobile phase.

scholarly journals Formulation and Quantitative Analysis of Betamethasone Valerate and Neomycin Sulfate Cream by High Performance Liquid Chromatography and Spectrophotometry

2019 ◽  
Vol 7 (22) ◽  
pp. 3841-3846
Author(s):  
Yade Metri Permata ◽  
Muchlisyam Bachri ◽  
Julia Reveny ◽  
Fitri Mardiyanti Sibuea
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Vitamin E ◽  
Stearic Acid ◽  
Mobile Phase ◽  
High Performance ◽  
Hplc Method ◽  
Betamethasone Valerate ◽  
Neomycin Sulfate

BACKGROUND: Combination of betamethasone valerate and neomycin sulfate in cream is used to treat the itching, redness, dryness, scaling, inflammation and discomfort of various skin conditions caused by infection. The combination of active ingredients has side effects which can cause dry skin, thinning of the skin, hypertrichosis, and stretch marks. AIM: The purpose of this study was to make a formula containing vitamin E and quantitative analysis of betamethasone valerate and neomycin sulfate in creams using High Performance Liquid Chromatography and Spectrophotometry Area Under Curve methods. METHODS: Cream preparation includes smelting and emulsification processes, with oil phases namely stearic acid and vitamin E as well as water phases are glycerin, sodium bi-borate, tri-ethanolamine. Physical tests for the cream were organoleptic, homogeneity, pH, evaluation of dispersion, and viscosity. HPLC analysis for cream was carried out using C18 column, and the mobile phase of methanol: water with comparison optimization beforehand. Spectrophotometry analysis for cream was carried out using application of Area Under Curves methods. RESULTS: The formula used was betamethasone valerate 5 mg, neomycin sulfate 25 mg, stearic acid, glycerin, sodium bi-borate, tri-ethanolamine, vitamin E and distilled water. The obtained cream was in the form of semi-solid, odorless, white (colorless), homogeneous, pH 7, the dispersion power of 500 mg cream is 4.0-4.3 cm in diameter and viscosity is 7500 Cps. Analysis of the determination of the levels of the two components was carried out by the HPLC method C-18 column with the mobile phase of methanol: water (90: 10). Betamethasone valerate and neomycin sulfate levels in formulas made HPLC methods were 94.15%, and 136.56%, respectively and using AUC spectrophotometry methods were 107.98% and 94.81%. CONCLUSION: Cream that made by new formula with vitamin E shows good result in physical evaluation. HPLC methods with a mobile phase of methanol: water (90:10) was not recommended, while the AUC spectrophotometry method shows the valid result of quantitative analysis of betamethasone valerate and neomycin sulfate in cream.

scholarly journals RAPID AND ECONOMICAL QUANTITATIVE DETERMINATION OF SEVERAL ANTIHYPERTENSIVE AGENTS IN PRESENCE OF HYDROCHLOROTHIAZIDE BY ISOCRATIC REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY IN THEIR PHARMACEUTICAL PREPARATIONS

2017 ◽  
Vol 10 (12) ◽  
pp. 155
Author(s):  
Panchumarthy Ravisankar ◽  
Devala Rao G ◽  
Md Shaheem Sulthana ◽  
Supriya K ◽  
Mounika G ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Mobile Phase ◽  
High Performance ◽  
Hplc Method ◽  
Control Analysis ◽  
Rp Hplc ◽  
Relative Standard

Objective: Objective of the present investigation is to develop a speedy isocratic reverse phase high-performance liquid chromatography (RP-HPLC) method for the separation and quantitative determination of 5 angiotensin II - receptor antagonists, namely, telmisartan, losartan, valsartan, olmesartan, irbesartan, and atenolol along with thiazide diuretics mostly hydrochlorothiazide (HCTZ).Methods: RP-HPLC method was evolved using Welchrom C18 column (4.6 × 250 mm, 5 μm) as a stationary phase with the mobile phase comprising a variety of phosphate buffer with pH-3.3 and acetonitrile in the proportion of 50:50 v/v. The mobile phase was pumped at a current rate of 1 mL/minute. The detection wavelength was carried out at 230 nm.Results: The total run time was 6 minutes and the elution window of only 3 minutes. The peaks were eluted with decorous resolution. The calibration curves were linear (r2=0.9998) in all cases. The percentage relative standard deviation (RSD%) was <2% and average recovery was above 99.95%. The method was validated specificity, precision, and accuracy. High recovery values and low RSD% prove that this method is very accurate and reproducible. The developed method was applied to the estimation of the above-said drugs in binary combinations from different manufacturers which were a good agreement with label claim.Conclusion: The important advantage of developed method was that the five individual drugs can be determined on a single chromatographic system without alteration in detection wavelength and mobile phase composition. This novel method was statistically validated as per ICH guidelines. The optimized method proved to be linear, accurate, and robust. Hence, the above said proposed method was found to be a rapid tool for the routine determination of the above-said drugs in alone or combination with HCTZ in quality control analysis without interference of excipients.

Analisis Kadar Zat Pewarna Tartrazin pada Minuman Ringan Berkarbonasi Khas Pekalongan dengan Metode High Performance Liquid Chromatography (HPLC)

2021 ◽  
Vol 1 ◽  
pp. 685-691
Author(s):  
AT Meta Lansamigi ◽  
Achmad Vandian Nur ◽  
W Wirasti ◽  
Khusna Santika Rahmasari
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Mobile Phase ◽  
High Performance ◽  
Hplc Method ◽  
Lemon Yellow ◽  
Colour Reaction ◽  
Auc Value ◽  
Carbonated Drinks

AbstractTartrazine is a coloring agent which is a mixture of phenolic compounds, polycyclic hydrocarbons, and heterocyclics. Because it dissolves easily in water, tartrazine is commonly used as a coloring agent in beverages. The addition of tartrazine in the drink aims to give it a more attractive color, namely lemon yellow. The purpose of this study was to analyze the tartrazine content in carbonated drinks and to determine whether the tartrazine content in the sample was in accordance with the BPOM Regulation No. 11 of 2019. Qualitative testing using colour reaction with FeSO4. Quantitative testing using the high performance liquid chromatography (HPLC) method with a mobile phase of acetonitrile, methanol and aquabides with a ratio (65,5 : 23,5 : 11). The data obtained is the AUC value. Data analisys using linear regression. The resul of qualitatif analysis with acolor test using FeSO4 reagent which produces a color until it is cloudy and is present on the surface. While the quanitative analysis using HPLC obtained the average levels in the oranfe sample 1,109 mg/kg and pineapple sample 0,933 mg/kg.Key words: Analysis, levels, tartrazine, dye, HPLC AbstrakTartrazin merupakan zat pewarna campuran dari senyawa fenol, hidrokarbon polisiklik, dan heterosiklik. Pada umumnya tartrazin digunakan sebagai bahan pewarna minuman karena mudah larut dalam air. Penambahan tartrazin dalam minuman bertujuan untuk memberikan warna yang lebih menarik yaitu kuning lemon. Tujuan dari penelitian ini adalah untuk menganalisis kandungan tartrazin dalam minuman berkarbonasi dan untuk mengetahui apakah kadar tartrazin dalam sampel sudah sesuai dengan peraturan BPOM No.11 Tahun 2019. Pengujian secara kualitatif dengan metode uji warna menggunakan FeSO4. Pengujian secara kuantitatif menggunakan metode High Performance Liquid Chromatography (HPLC) dengan fase gerak asetonitril, metanol, dan aquabides dengan perbandingan (65,5 : 23,5 : 11). Data yang didapat adalah nilai AUC. Analisis data menggunakan regresi linier. Hasil dari analisis kualitataif dengan uji warna menggunakan reagen FeSO4 yang menghasilkan warna sampel keruh dan terdapat endapan. Sedangkan pada analisis kuantitif menggunakan HPLC didapatkan rata-rata kadar pada sampel rasa jeruk 1,109 mg/kg dan pada sampel nanas 0,933 mg/kg.Kata kunci : Analisis, kadar, tartrazin, pewarna, HPLC

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