Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay
Keyword(s):
The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation.
2007 ◽
Vol 9
(1)
◽
pp. 42-46
◽
2008 ◽
Vol 10
(5)
◽
pp. 435-441
◽
2013 ◽
Vol 61
(5)
◽
pp. 1060-1066
◽
Keyword(s):
2006 ◽
Vol 54
(4)
◽
pp. 1158-1165
◽
Keyword(s):
2007 ◽
Vol 25
(9)
◽
pp. 1048-1053
◽
2017 ◽
Vol 241
◽
pp. 58-63
◽
Keyword(s):
2010 ◽
Vol 12
(4)
◽
pp. 512-519
◽