scholarly journals Reassortment of bluetongue virus vaccine serotypes in cattle

2018 ◽  
Vol 11 ◽  
Author(s):  
Carien van den Bergh ◽  
Peter Coetzee ◽  
Estelle H. Venter
Keyword(s):  
Virus Vaccine ◽  
Bluetongue Virus ◽  
Vaccine Serotypes

scholarly journals Reassortment of bluetongue virus vaccine serotypes in cattle

2018 ◽  
Vol 89 ◽  
Author(s):  
Carien Van den Bergh ◽  
Peter Coetzee ◽  
Estelle H. Venter
Keyword(s):  
South Africa ◽  
Virus Vaccine ◽  
Bluetongue Virus ◽  
Field Isolate ◽  
Vero Cells ◽  
Buffy Coat ◽  
Post Inoculation ◽  
Vaccine Serotypes ◽  
Segment 8 ◽  
Modified Live Vaccine

Bluetongue is primarily a disease of sheep in South Africa, while cattle and goats are mostly subclinically infected. The viraemia of bluetongue virus in cattle lasts much longer than in sheep and the role of cattle in the epidemiology of bluetongue in South Africa is poorly understood. Bluetongue virus has a segmented double-stranded ribonucleic acid genome and reassortment of genomes is a common feature. The aim of the study was to investigate whether reassortment occurs between vaccine and field strains when simultaneously administered to cattle. Six cattle between the ages of 6 and 12 months were infected with five strains of modified live vaccine bluetongue virus and a virulent field isolate of bluetongue virus 4. Blood samples were subsequently collected daily from these animals from day 1 to day 39 post-inoculation. Viruses were directly isolated during viraemia from the buffy coat on Vero cells using the plaque forming unit method. Analysis of plaques indicated that no reassortants between virulent field and vaccine strains occurred and the virulent bluetongue virus 4 was identified as the predominant virus strain. However, a reassortant virus between two bluetongue virus vaccine strains was isolated from the buffy coat. Whole genome sequences from the vaccine viruses were compared to the suspected reassortant and it was found that segment 8 exchanged between the bluetongue virus 8 and bluetongue virus 9 vaccine strains. The use of the live-attenuated bluetongue virus multivalent vaccine in South Africa causes circulation of different vaccine serotypes in Culicoides spp. and susceptible hosts and cattle might provide the ideal host for reassortment to occur.

scholarly journals Bovine viral diarrhea in captive Rocky Mountain bighorn sheep associated with administration of a contaminated modified-live bluetongue virus vaccine

2018 ◽  
Vol 31 (1) ◽  
pp. 107-112 ◽  
Author(s):  
Karen A. Fox ◽  
Jennifer H. Kopanke ◽  
Justin S. Lee ◽  
Lisa L. Wolfe ◽  
Kristy L. Pabilonia ◽  
...  
Keyword(s):  
Virus Vaccine ◽  
Bluetongue Virus ◽  
Rocky Mountain ◽  
Late Summer ◽  
Bighorn Sheep ◽  
Bovine Viral Diarrhea ◽  
Lymphoid Tissues ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Gastrointestinal Lesions

In late summer 2017, we observed acute, fatal cases of bovine viral diarrhea in captive Rocky Mountain bighorn sheep ( Ovis canadensis canadensis) in Colorado following use of a contaminated modified-live bluetongue virus vaccine. Following vaccination, at least 14 of 28 (50%) vaccinated bighorn sheep developed hemorrhagic diarrhea, and 6 of 28 (21%) vaccinated bighorn sheep died. Autopsy findings were predominantly necroulcerative-to-necrohemorrhagic gastrointestinal lesions. Less frequent lesions included suffusive hemorrhages of serosal surfaces of abdominal viscera, and lymphoid necrosis in gut-associated lymphoid tissues. All of the 6 bighorn sheep that died were positive on real-time PCR (rtPCR) for bovine viral diarrhea virus (BVDV) in multiple tissues. Seroconversion to BVDV-1 and immunohistochemistry for BVDV in affected tissues confirmed rtPCR results. Next-generation sequencing confirmed a match between the infecting strain of BVDV-1b and the contaminated vaccine.

scholarly journals Bluetongue virus vaccine: conventional to modern approach

2019 ◽  
Vol 63 (01) ◽  
pp. 3-18 ◽  
Author(s):  
K. RANJAN ◽  
M. PRASAD ◽  
B. BRAR ◽  
U. LAMBE ◽  
R. KUMAR ◽  
...  
Keyword(s):  
Virus Vaccine ◽  
Bluetongue Virus ◽  
Modern Approach

scholarly journals Expected Net Benefit of Vaccinating Rangeland Sheep against Bluetongue Virus Using a Modified-Live versus Killed Virus Vaccine

2017 ◽  
Vol 4 ◽  
Author(s):  
Tristram R. Munsick ◽  
Dannele E. Peck ◽  
John P. Ritten ◽  
Randall Jones ◽  
Michelle Jones ◽  
...  
Keyword(s):  
Virus Vaccine ◽  
Bluetongue Virus ◽  
Net Benefit

A review of potential bluetongue virus vaccine strategies

2017 ◽  
Vol 206 ◽  
pp. 84-90 ◽  
Author(s):  
Christie Mayo ◽  
Justin Lee ◽  
Jennifer Kopanke ◽  
N. James MacLachlan
Keyword(s):  
Virus Vaccine ◽  
Bluetongue Virus

Recombinant canarypox virus vaccine co-expressing genes encoding the VP2 and VP5 outer capsid proteins of bluetongue virus induces high level protection in sheep

Vaccine ◽  
2007 ◽  
Vol 25 (4) ◽  
pp. 672-678 ◽  
Author(s):  
Josh D. Boone ◽  
Udeni B. Balasuriya ◽  
Kemal Karaca ◽  
Jean-Christophe Audonnet ◽  
Jiansheng Yao ◽  
...  
Keyword(s):  
Virus Vaccine ◽  
Bluetongue Virus ◽  
Capsid Proteins ◽  
Genes Encoding ◽  
High Level ◽  
Outer Capsid ◽  
Canarypox Virus

scholarly journals Complete Genome Sequences of Virus Strains Isolated from Bottle A of the South African Live Attenuated Bluetongue Virus Vaccine

2020 ◽  
Vol 9 (22) ◽  
Author(s):  
Peter Coetzee ◽  
Alan J. Guthrie ◽  
Karen Ebersohn ◽  
James N. Maclachlan ◽  
Arshad Ismail ◽  
...  
Keyword(s):  
South African ◽  
Virus Vaccine ◽  
Complete Genome ◽  
Bluetongue Virus ◽  
The South ◽  
Genome Sequences ◽  
Biological Products ◽  
Virus Strains

ABSTRACT This is a report of the complete genome sequences of plaque-selected isolates of five virus strains included in bottle A of the South African Onderstepoort Biological Products commercial live attenuated bluetongue virus vaccine.

Sign in / Sign up

Export Citation Format

Share Document