scholarly journals Red blood cell volume as a predictor of fatal reactions in cattle infected with Theileria parva Katete

2007 ◽  
Vol 74 (1) ◽  
Author(s):  
P. Fandamu ◽  
T. Marcotty ◽  
J.R.A. Brandt ◽  
L. Duchateau ◽  
N. Speybroeck ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Cell Volume ◽  
Red Blood Cell ◽  
Packed Cell Volume ◽  
Blood Cells ◽  
Mean Corpuscular Volume ◽  
Corpuscular Volume ◽  
Theileria Parva ◽  
Fatal Reaction

A comparison of mean corpuscular volume (MCV) and packed cell volume (PCV) was made between cattle undergoing lethal and non-lethal reactions following experimental infections with the apicomplexan protozoa, Theileria parva Katete. This work confirmed that anaemia occurs in infected animals. However, the fall in PCV was steeper in lethal reactions compared to non-lethal reactions. Our results show that animals with initially lower MCV values are more prone to fatal reaction, despite having normal PCV profiles. The study also found that small red blood cells are more likely to be infected with T. parva. These findings suggest that animals with a higher proportion of small red blood cells in circulation will be more likely to succumb to T. parva infections. The potential for using MCV as a predictor of the outcome of infection challenge is discussed.

Blood flow and volume distribution during forced submergence in Pekin ducks (Anas platyrhynchos)

1988 ◽  
Vol 66 (7) ◽  
pp. 1589-1596 ◽  
Author(s):  
M. R. A. Heieis ◽  
David R. Jones
Keyword(s):  
Blood Flow ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Cell Volume ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Area Measurement ◽  
Volume Distribution ◽  
Residual Blood ◽  
Time Required

Blood is the major oxygen store in ducks forced to dive, and underwater endurance depends on how much of this store can be used by oxygen-sensitive tissues such as the heart and brain. Arterial injection of macroaggregated albumin labelled with technetium-99 m, which is trapped and held by capillaries, showed that circulation in dives was restricted to the thoracic and head areas. However, tracing red blood cells labelled with technetium-99 m as they were injected during dives showed not only that the time required for the activity to reach equilibrium was 4–10 times longer than when labelled cells were injected into resting ducks but also that blood flow continued in the leg and visceral regions. Tracing red blood cells, labelled with technetium-99 m and mixed in the circulation before a dive, during the dive showed that labelled red blood cells were redistributed from the peripheral and visceral areas to the central cardiovascular area. Measurement of circulating red blood cell volume during and after diving showed that, on average, 75.24 ± 4.56% of the total red blood cell volume was circulated during forced submergence. Hence, in forced dives, red blood cell volume is positioned in such a manner that the heart and brain have access to the oxygen stored there, and the residual blood flow in the periphery ensures that most of the red blood cell volume is circulated.

Red blood cell volume and distribution before and after bleed-out in the rat

1960 ◽  
Vol 198 (6) ◽  
pp. 1177-1180 ◽  
Author(s):  
Robert J. Dellenback ◽  
Gerhard H. Muelheims
Keyword(s):  
Body Weight ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Cell Volume ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Before And After ◽  
Negligible Contribution

The distribution of red blood cells in nine normal Nembutalized rats (323.2–415.0 gm body weight) was determined by the Cr51-labeled red blood cell technique. Microliters of red blood cells per total and per gram of tissue are reported for the testes, brain, intestine, kidney, heart-lung, spleen, liver, bone, muscle and skin. Values are also listed for the same organs and tissues determined after rapid bleed-out as found by Muelheims, Dellenback and Rawson. A comparison of these values shows that the liver, heart-lung and muscle contribute approximately 80% of all red blood cells removed in the hemorrhage. The skin, bone, kidney and intestine contribute as a group the remaining 20% with a negligible contribution from the testes and brain and no contribution from the spleen.

Red blood cells do not contribute to removal of K+ released from exhaustively working forearm muscle

1998 ◽  
Vol 85 (1) ◽  
pp. 326-332 ◽  
Author(s):  
N. Maassen ◽  
M. Foerster ◽  
H. Mairbäurl
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Cell Volume ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Plasma Osmolality ◽  
Cell Water ◽  
Forearm Exercise ◽  
Hb Concentration ◽  
Forearm Muscle

K+ released from exercising muscle via K+ channels needs to be removed from the interstitium into the blood to maintain high muscle cell membrane potential and allow normal muscle contractility. Uptake by red blood cells has been discussed as one mechanism that would also serve to regulate red blood cell volume, which was found to be constant despite increased plasma osmolality and K+ concentration ([[Formula: see text]]). We evaluated exercise-related changes in [[Formula: see text]], pH, osmolality, mean cellular Hb concentration, cell water, and red blood cell K+ concentration during exhaustive handgrip exercise. Unidirectional86Rb+(K+) uptake by red blood cells was measured in media with elevated extracellular K+, osmolarity, and catecholamines to simulate particularly those exercise-related changes in plasma composition that are known to stimulate K+ uptake. During exercise [[Formula: see text]] increased from 4.4 ± 0.7 to 7.1 ± 0.5 mmol/l plasma water and red blood cell K+ concentration increased from 137.2 ± 6.0 to 144.6 ± 4.6 mmol/l cell water ( P ≤ 0.05), but the intracellular K+-to-mean cellular Hb concentration ratio did not change.86Rb+uptake by red blood cells was increased by ∼20% on stimulation, caused by activation of the Na+-K+pump and Na+-K+-2Cl−cotransport. Results indicate the K+ content of red blood cells did not change as cells passed the exhaustively exercising forearm muscle despite the elevated [[Formula: see text]]. The tendency for an increase in intracellular K+ concentration was due to a slight, although statistically not significant, decrease in red blood cell volume. K+ uptake, although elevated, was too small to move significant amounts of K+ into red blood cells. Our results suggest that red blood cells do not contribute to the removal of K+ released from muscle and do not regulate their volume by K+uptake during exhaustive forearm exercise.

Red blood cell volume and distribution after bleed-out in the rat as determined by Cr51-labeled red blood cells

1958 ◽  
Vol 196 (1) ◽  
pp. 169-172 ◽  
Author(s):  
Gerhard Muelheims ◽  
Robert Dellenback ◽  
Ruth Rawson
Keyword(s):  
Body Weight ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Cell Volume ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Total Tissue

The distribution of red blood cells after bleed-out was determined in 20 Nembutalized rats (307.1–484.4 gm body weight), using Cr51-labeled red blood cells. The normal red blood cell volume was 2.20 ± 0.25 ml/100 gm body weight. On the average 68.5% of the red blood cells were removed by the bleed-out. Per total tissue the muscle contained most of the red blood cell volume remaining after bleed-out and together with the liver and bone about 65%. Per gram of tissue the spleen had the highest red blood cell volume after bleed-out. The volume of red blood cells remaining in the spleen and kidney was the same as found by Riecke and Everett in unbled Nembutalized rats.

scholarly journals Red blood cell volume can be independently determined in vitro using sheep and human red blood cells labeled at different densities of biotin

Transfusion ◽  
2009 ◽  
Vol 49 (6) ◽  
pp. 1178-1185 ◽  
Author(s):  
Donald M. Mock ◽  
Nell I. Matthews ◽  
Ronald G. Strauss ◽  
Leon F. Burmeister ◽  
Robert Schmidt ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Cell Volume ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Human Red Blood Cells

Effects of lost surface area on red blood cells and red blood cell survival in mice

1996 ◽  
Vol 271 (6) ◽  
pp. C1847-C1852 ◽  
Author(s):  
R. E. Waugh ◽  
I. H. Sarelius
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Cell Survival ◽  
Cell Volume ◽  
Surface Area ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Cell Area ◽  
Cell Age ◽  
The Mean

The effects of removing area from mouse red blood cells on the fate of the cells after reinfusion were investigated. When cells were made nearly spherical (by reducing cell area by approximately 35%) and then reinfused into the animal, most were cleared from the circulation within 1-2 h, although approximately 20% of the cells survived for 4 h or longer. When only 20% of the area was removed (leaving a 15% excess), more than 90% of the cells continued to circulate for 4 h. After reinfusion, the mean surface area of the surviving cells remained constant (73-75 microns2), but the mean volume decreased, from 56.6 +/- 2.1 to 19.1 +/- 1.5 microns3 (+/- SD of 5 replicates) over 4 h. These changes did not occur in cells suspended in plasma but not reinfused into the animal. Thus a loss of surface area results in a decrease in cell volume, as if to maintain a requisite degree of deformability. The results support the hypothesis that the increase in cell density associated with increasing cell age may be a consequence of surface area loss.

scholarly journals pH regulatory Na/H exchange by Amphiuma red blood cells.

1994 ◽  
Vol 103 (6) ◽  
pp. 1035-1053 ◽  
Author(s):  
P M Cala ◽  
H M Maldonado
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Cell Volume ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Ph Sensing ◽  
Cell Shrinkage ◽  
Set Point ◽  
Degree Of Acidification ◽  
Regulatory Functions

In Amphiuma red blood cells, the Na/H exchanger has been shown to play a central role in the regulation of cell volume following cell shrinkage (Cala, P. M. 1980. Journal of General Physiology. 76:683-708.) The present study was designed to evaluate the existence of pH regulatory Na/H exchange in the Amphiuma red blood cell. The data illustrate that when the intracellular pHi was decreased below the normal value of 7.00, Na/H exchange was activated in proportion to the degree of acidification. Once activated, net Na/H exchange flux persisted until normal intracellular pH (6.9-7.0) was restored, with a half time of approximately 5 min. These observations established a pHi set point of 7.00 for the pH-activated Na/H exchange of Amphiuma red blood cell. This is in contrast to the behavior of osmotically shrunken Amphiuma red blood cells in which no pHi set point could be demonstrated. That is, when activated by cell shrinkage the Na/H exchange mediated net Na flux persisted until normal volume was restored regardless of pHi. In contrast, when activated by cell acidification, the Na/H exchanger functioned until pHi was restored to normal and cell volume appeared to have no effect on pH-activated Na/H exchange. Studies evaluating the kinetic and inferentially, the molecular equivalence of the volume and pHi-induced Amphiuma erythrocyte Na/H exchanger(s), indicated that the apparent Na affinity of the pH activated cells is four times greater than that of shrunken cells. The apparent Vmax is also higher (two times) in the pH activated cells, suggesting the involvement of two distinct populations of the transporter in pH and volume regulation. However, when analyzed in terms of a bisubstrate model, the same data are consistent with the conclusion that both pH and volume regulatory functions are mediated by the same transport protein. Taken together, these data support the conclusion that volume and pH are regulated by the same effector (Na/H exchanger) under the control of as yet unidentified, distinct and cross inhibitory volume and pH sensing mechanisms.

Microfluidic electrical impedance assessment of red blood cell mediated microvascular occlusion

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yuncheng Man ◽  
Debnath Maji ◽  
Ran An ◽  
Sanjay Ahuja ◽  
Jane A Little ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Electrical Impedance ◽  
Cell Disease ◽  
Blood Disorders

Alterations in the deformability of red blood cells (RBCs), occurring in hemolytic blood disorders such as sickle cell disease (SCD), contributes to vaso-occlusion and disease pathophysiology. However, there are few...

Sign in / Sign up

Export Citation Format

Share Document