In vivo sectional imaging of the retinal periphery using conventional optical coherence tomography systems

Abstract Background and study aims First-generation optical coherence tomography (OCT) has been shown to increase diagnostic sensitivity for malignant biliary and pancreatic-duct strictures. A newer OCT imaging system, NVision Volumetric Laser Endomicroscopy (VLE), allows for in vivo cross-sectional imaging of the ductal wall at the microstructure level during endoscopic retrograde cholangiopancreatography (ERCP). The aim of this study was to identify and evaluate characteristics on OCT that are predictive of benign and malignant strictures. Patients and methods Consecutive patients from six centers who underwent OCT between September 2016 and September 2017 were included in a dedicated registry. OCT images were analyzed, and nine recurring characteristics were further assessed. Final diagnosis was based on histology and/or surgical pathology. Results 86 patients were included (49 % male, mean age 64.7). OCT was performed in the bile duct in 79 patients and the pancreatic duct in seven. Nine OCT characteristics were identified: dilated hypo-reflective structures (n = 7), onion-skin layering (n = 8), intact layering (n = 17), layering effacement (n = 25), scalloping (n = 20), thickened epithelium (n = 42), hyper-glandular mucosa (n = 13), prominent blood vessels (n = 6), and a hyper-reflective surface (n = 20). Presence of hyper-glandular mucosa, hyper-reflective surface and scalloping significantly increased the odds of malignancy diagnosis by 6 times more (P = 0.0203; 95 % CI 1.3 to 26.5), 4.7 times more (P = 0.0255; 95 % CI 1.2 to 18.0) and 7.9 times more (P = 0.0035; 95 % CI 1.97 to 31.8) respectively. Conclusion By providing in-vivo cross-sectional imaging of the pancreatic and biliary duct wall, OCT technology may improve sensitivity in diagnosing malignant strictures and provide standardizable criteria predictive of malignancy.

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Noninvasive cross-sectional imaging of proximal caries using swept-source optical coherence tomography (SS-OCT)in vivo

Journal of Biophotonics ◽

10.1002/jbio.201200210 ◽

2013 ◽

Vol 7 (7) ◽

pp. 506-513 ◽

Cited By ~ 50

Author(s):

Yasushi Shimada ◽

Hisaichi Nakagawa ◽

Alireza Sadr ◽

Ikumi Wada ◽

Masatoshi Nakajima ◽

...

Keyword(s):

Optical Coherence Tomography ◽

Optical Coherence ◽

Cross Sectional ◽

Swept Source ◽

Proximal Caries ◽

Cross Sectional Imaging ◽

Sectional Imaging

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In vivo cross-sectional imaging of the phonating larynx using long-range Doppler optical coherence tomography

Scientific Reports ◽

10.1038/srep22792 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 17

Author(s):

Carolyn A. Coughlan ◽

Li-dek Chou ◽

Joseph C. Jing ◽

Jason J. Chen ◽

Swathi Rangarajan ◽

...

Keyword(s):

Optical Coherence Tomography ◽

Long Range ◽

Optical Coherence ◽

Cross Sectional ◽

Cross Sectional Imaging ◽

Sectional Imaging ◽

Doppler Optical Coherence Tomography

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258: Diagnosis and Intraoperative Staging of Bladder Disease by in-vivo Optical Coherence Tomography (OCT)

The Journal of Urology ◽

10.1016/s0022-5347(18)37520-7 ◽

2004 ◽

Vol 171 (4S) ◽

pp. 68-68 ◽

Cited By ~ 2

Author(s):

Markus D. Sachs ◽

Dmitry Daniltchenko ◽

Eva Lankenau ◽

Frank Koenig ◽

Gerion Huettmann ◽

...

Keyword(s):

Optical Coherence Tomography ◽

Optical Coherence ◽

Bladder Disease

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Evaluating Retinal and Choroidal Perfusion Changes after Isometric and Dynamic Activity Using Optical Coherence Tomography Angiography

Diagnostics ◽

10.3390/diagnostics11050808 ◽

2021 ◽

Vol 11 (5) ◽

pp. 808

Author(s):

Max Philipp Brinkmann ◽

Nikolas Xavier Kibele ◽

Michelle Prasuhn ◽

Vinodh Kakkassery ◽

Mario Damiano Toro ◽

...

Keyword(s):

Optical Coherence Tomography ◽

Optical Coherence Tomography Angiography ◽

Isometric Exercise ◽

Dynamic Exercise ◽

Exercise Heart Rate ◽

Optical Coherence ◽

Dynamic Activity ◽

Capillary Plexus ◽

Choroidal Perfusion

Optical coherence tomography angiography (OCTA) is a non-invasive tool for imaging and quantifying the retinal and choroidal perfusion state in vivo. This study aimed to evaluate the acute effects of isometric and dynamic exercise on retinal and choroidal sublayer perfusion using OCTA. A pilot study was conducted on young, healthy participants, each of whom performed a specific isometric exercise on the first day and a dynamic exercise the day after. At baseline and immediately after the exercise, heart rate (HR), mean arterial pressure (MAP), superficial capillary plexus perfusion (SCPP), deep capillary plexus perfusion (DCPP), choriocapillaris perfusion (CCP), Sattlers’s layer perfusion (SLP), and Haller’s layer perfusion (HLP) were recorded. A total of 34 eyes of 34 subjects with a mean age of 32.35 ± 7.87 years were included. HR as well as MAP increased significantly after both types of exercise. Both SCPP and DCPP did not show any significant alteration due to isometric or dynamic exercise. After performing dynamic exercise, CCP, SLP, as well as HLP significantly increased. Changes in MAP correlated significantly with changes in HLP after the dynamic activity. OCTA-based analysis in healthy adults following physical activity demonstrated a constant retinal perfusion, supporting the theory of autoregulatory mechanisms. Dynamic exercise, as opposed to isometric activity, significantly changed choroidal perfusion. OCTA imaging may represent a novel and sensitive tool to expand the diagnostic spectrum in the field of sports medicine.

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Quantitative Swept-Source Optical Coherence Tomography of Early Enamel Erosion in vivo

Caries Research ◽

10.1159/000477098 ◽

2017 ◽

Vol 51 (4) ◽

pp. 410-418 ◽

Cited By ~ 5

Author(s):

Rupert S. Austin ◽

Maisalamah Haji Taha ◽

Frederic Festy ◽

Richard Cook ◽

Manoharan Andiappan ◽

...

Keyword(s):

Optical Coherence Tomography ◽

Healthy Volunteers ◽

Orange Juice ◽

Signal Intensity ◽

Surface States ◽

Enamel Surface ◽

Optical Coherence ◽

Swept Source ◽

Water Rinsing

Swept-source optical coherence tomography (SS-OCT) shows potential for the in vivo quantitative evaluation of micro-structural enamel surface phenomena occurring during early erosive demineralization. This randomized controlled single-blind cross-over clinical study aimed to evaluate the use of SS-OCT for detecting optical changes in the enamel of 30 healthy volunteers subjected to orange juice rinsing (erosive challenge) in comparison to mineral water rinsing (control), according to wiped and non-wiped enamel surface states. Participants were randomly allocated to 60 min of orange juice rinsing (pH 3.8) followed by 60 min of water rinsing (pH 6.7) and vice versa, with a 2-week wash-out period. In addition, the labial surfaces of the right or left maxillary incisors were wiped prior to SS-OCT imaging. An automated ImageJ algorithm was designed to analyse the back-scattered OCT signal intensity (D) after orange juice rinsing compared to after water rinsing. D was quantified as the OCT signal scattering from the 33 µm sub-surface enamel, normalised by the total OCT signal intensity entering the enamel. The back-scattered OCT signal intensity increased by 3.1% (95% CI 1.1-5.1%) in the wiped incisors and by 3.5% (95% CI 1.5-5.5%) in the unwiped incisors (p < 0.0001). Wiping reduced the back-scattered OCT signal intensity by 1.7% (95% CI -3.2 to -0.3%; p = 0.02) in comparison to the unwiped enamel surfaces for both rinsing solutions (p = 0.2). SS-OCT detected OCT signal changes in the superficial sub-surface enamel of maxillary central incisor teeth of healthy volunteers after orange juice rinsing.

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In vivo cellular optical coherence tomography imaging

Nature Medicine ◽

10.1038/nm0798-861 ◽

1998 ◽

Vol 4 (7) ◽

pp. 861-865 ◽

Cited By ~ 194

Author(s):

Stephan A. Boppart ◽

Brett E. Bouma ◽

Costas Pitris ◽

James F. Southern ◽

Mark E. Brezinski ◽

...

Keyword(s):

Optical Coherence Tomography ◽

Optical Coherence ◽

Tomography Imaging ◽

Optical Coherence Tomography Imaging

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Optimization of X-ray Investigations in Dentistry Using Optical Coherence Tomography

Sensors ◽

10.3390/s21134554 ◽

2021 ◽

Vol 21 (13) ◽

pp. 4554

Author(s):

Ralph-Alexandru Erdelyi ◽

Virgil-Florin Duma ◽

Cosmin Sinescu ◽

George Mihai Dobre ◽

Adrian Bradu ◽

...

Keyword(s):

Optical Coherence Tomography ◽

Radiation Dose ◽

Imaging Techniques ◽

Image Resolution ◽

Optical Coherence ◽

X Rays ◽

High Quality ◽

X Ray

The most common imaging technique for dental diagnoses and treatment monitoring is X-ray imaging, which evolved from the first intraoral radiographs to high-quality three-dimensional (3D) Cone Beam Computed Tomography (CBCT). Other imaging techniques have shown potential, such as Optical Coherence Tomography (OCT). We have recently reported on the boundaries of these two types of techniques, regarding. the dental fields where each one is more appropriate or where they should be both used. The aim of the present study is to explore the unique capabilities of the OCT technique to optimize X-ray units imaging (i.e., in terms of image resolution, radiation dose, or contrast). Two types of commercially available and widely used X-ray units are considered. To adjust their parameters, a protocol is developed to employ OCT images of dental conditions that are documented on high (i.e., less than 10 μm) resolution OCT images (both B-scans/cross sections and 3D reconstructions) but are hardly identified on the 200 to 75 μm resolution panoramic or CBCT radiographs. The optimized calibration of the X-ray unit includes choosing appropriate values for the anode voltage and current intensity of the X-ray tube, as well as the patient’s positioning, in order to reach the highest possible X-rays resolution at a radiation dose that is safe for the patient. The optimization protocol is developed in vitro on OCT images of extracted teeth and is further applied in vivo for each type of dental investigation. Optimized radiographic results are compared with un-optimized previously performed radiographs. Also, we show that OCT can permit a rigorous comparison between two (types of) X-ray units. In conclusion, high-quality dental images are possible using low radiation doses if an optimized protocol, developed using OCT, is applied for each type of dental investigation. Also, there are situations when the X-ray technology has drawbacks for dental diagnosis or treatment assessment. In such situations, OCT proves capable to provide qualitative images.

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Multi-center clinical study using optical coherence tomography for evaluation of cervical lesions in-vivo

Scientific Reports ◽

10.1038/s41598-021-86711-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Chenchen Ren ◽

Xianxu Zeng ◽

Zhongna Shi ◽

Chunyan Wang ◽

Huifen Wang ◽

...

Keyword(s):

Optical Coherence Tomography ◽

Real Time ◽

Precancerous Lesions ◽

Diagnostic Value ◽

Optical Coherence ◽

Cervical Lesions ◽

In Vivo Evaluation ◽

Histological Sections ◽

Histological Images

AbstractIn this prospective study of an in-vivo cervical examination using optical coherence tomography (OCT), we evaluated the diagnostic value of non-invasive and real-time OCT in cervical precancerous lesions and cancer diagnosis, and determined the characteristics of OCT images. 733 patients from 5 Chinese hospitals were inspected with OCT and colposcopy-directed biopsy. The OCT images were compared with the histological sections to find out the characteristics of various categories of lesions. The OCT images were also interpreted by 3 investigators to make a 2-class classification, and the results were compared against the pathological results. Various structures of the cervical tissue were clearly observed in OCT images, which matched well with the corresponding histological sections. The OCT diagnosis results delivered a sensitivity of 87.0% (95% confidence interval, CI 82.2–90.7%), a specificity of 84.1% (95% CI 80.3–87.2%), and an overall accuracy of 85.1%. Both good consistency of OCT images and histological images and satisfactory diagnosis results were provided by OCT. Due to its features of non-invasion, real-time, and accuracy, OCT is valuable for the in-vivo evaluation of cervical lesions and has the potential to be one of the routine cervical diagnosis methods.

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