Identification of a New Single-nucleotide Polymorphism within the Apolipoprotein A5 Gene, Which is Associated with Metabolic Syndrome

The relationship between nitric oxide production and metabolic disorders and the role of endothelial nitric oxide synthase (eNOS or NOS3) in metabolic syndrome (MS) remain poorly understood and need deeper investigation. In this context the role of the NOS3 gene in pathogenesis of MS is of special interest. The aim of the study was to investigate association of NOS3 single nucleotide polymorphism C774T with risk of MS in the Slavic population of the Kaliningrad region and the relationship of this polymorphic variant with some parameters of endothelial dysfunction. The study included 128 patients (48 men and 80 women aged from 36 to 52 years) with MS. The control group consisted of 126 healthy volunteers (60 men and 66 women aged from 30 to 40 years). Genotyping was performed by real-time PCR. Serum nitrite levels were determined spectrophotometrically by the Griess method. Serum levels of endothelin-1 and eNOS were evaluated by ELISA. The study has shown association of T allele (OR=2.06; p=0.0004; CI: 1.38-3.08) and CT genotype (OR=1.97; p=0.014; CI: 1.14-3.40 ) C774T polymorphism of the NOS3 gene with risk of MS in the Slavic population of the Kaliningrad region. Allele C (OR=0.48; p=0.0004; CI: 0.32-0.72) and homozygous CC genotype (OR=0.41; p=0.001; CI: 0.24-0,69) C774T polymorphism of the NOS3 gene were associated with reduced risk of the development of MS. Significant differences in serum levels of eNOS and endothelin-1 depended on the CT and TT genotypes of C774T polymorphism of the NOS3 gene in MS.

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Association of single-nucleotide polymorphism (SNP) in the 5-alpha-reductase gene (SRD5A2) with increased prevalence of metabolic syndrome in chemotherapy-treated testicular cancer (TC) survivors.

Journal of Clinical Oncology ◽

10.1200/jco.2011.29.15_suppl.4564 ◽

2011 ◽

Vol 29 (15_suppl) ◽

pp. 4564-4564

Author(s):

H. Boer ◽

C. Meijer ◽

J. D. Dijck-Brouwer ◽

I. P. Kema ◽

J. D. Lefrandt ◽

...

Keyword(s):

Metabolic Syndrome ◽

Single Nucleotide Polymorphism ◽

Testicular Cancer ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Reductase Gene

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A Single Nucleotide Polymorphism -1131T>C in the Apolipoprotein A5 Gene Modulates the Levels of Triglyceride

Laboratory Medicine ◽

10.1309/lm3fqygrw64htinw ◽

2011 ◽

Vol 42 (3) ◽

pp. 171-174 ◽

Cited By ~ 1

Author(s):

Somayyeh Haqparast ◽

Mahdi Fasihi Ramandi ◽

Ali Samadikuchaksaraei ◽

Vahid Pazhakh

Keyword(s):

Single Nucleotide Polymorphism ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Apolipoprotein A5

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Polymorphism in the SIRT1 gene and parameters of metabolic syndrome in a sample of the adult Brazilian population

Revista de Nutrição ◽

10.1590/1678-98652016000100001 ◽

2016 ◽

Vol 29 (1) ◽

pp. 1-10 ◽

Cited By ~ 2

Author(s):

Marina Veloso de Oliveira MENEGUETTE ◽

Camila Andréa de OLIVEIRA ◽

Maria Helena de Melo LIMA ◽

Kathleen Nicole PINA ◽

Maria Esméria Corezola do AMARAL

Keyword(s):

Metabolic Syndrome ◽

Single Nucleotide Polymorphism ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Overweight And Obesity ◽

Brazilian Population ◽

Low Density ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

The Metabolic Syndrome

ABSTRACT Objective: To evaluate whether the single nucleotide polymorphism rs7895833 (A/G) of the gene SIRT1 is associated with metabolic syndrome criteria in a sample of Brazilian adults. Methods: Serum samples and oral mucosal cells were collected from 243 subjects aged 30 to 70 years. Biochemical, hormonal, and anthropometric data were obtained. The single nucleotide polymorphism rs7895833 (A/G) was analyzed by polymerase chain reaction using the amplification refractory mutation system. Results: Among the 243 study subjects, 100 (41.15%) were classified as non-metabolic syndrome and 143 (58.85%), as metabolic syndrome. The frequency of the single nucleotide polymorphism rs7895833 (A/G) did not differ between the groups. However, 111 patients (45.67%) were overweight (body mass index: 25-29.9 kg/m2). Blood glucose, total cholesterol, triglycerides, very low density lipoprotein, low density lipoprotein, waist and hip circumferences, and blood pressure were higher in the metabolic syndrome group than in the non-metabolic syndrome group. Free thyroxine 4, grown hormone, and insulin levels were within the normal range. The metabolic conditions of the patients with metabolic syndrome indicate biochemical, anthropometric, and hormonal changes characteristic of overweight and obesity. Conclusion: The SIRT1 polymorphism rs7895833 (A/G) is not associated with the metabolic syndrome in the adult Brazilian population.

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