scholarly journals Irradiating stored blood and storing irradiated blood: Is it different? - A study of serial changes in biochemical parameters of red blood cell units

2021 ◽  
Vol 15 (2) ◽  
pp. 172
Author(s):  
Abhishekh Basavarajegowda ◽  
Pramanya Balasubramanyam ◽  
Nandeesha Hanumanthappa ◽  
VirSingh Negi ◽  
KT Harichandrakumar
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Biochemical Parameters ◽  
Stored Blood

Packed Red Blood Cell Transfusions in Critically Ill Patients

2011 ◽  
Vol 31 (1) ◽  
pp. 25-34 ◽  
Author(s):  
Tara Ann Collins
Keyword(s):  
Blood Cell ◽  
Critically Ill ◽  
Red Blood Cell ◽  
Critically Ill Patients ◽  
Critical Review ◽  
Red Blood Cell Transfusion ◽  
Blood Transfusions ◽  
Change In Practice ◽  
Red Blood Cell Transfusions ◽  
Stored Blood

Anemia, which is prevalent in critically ill patients, often requires frequent blood transfusions. These blood transfusions are not without risks. A critical review of 6 studies shows an association between red blood cell transfusion and increased mortality. However, when disease state was adjusted for in 2 studies, researchers found that red blood cell transfusion correlated with decreased mortality. Thus further research, particularly on leukoreduction and age of stored blood, must be done before a change in practice can be implemented. It is vital that nurses stay current on this research in order to improve patients’ outcomes.

Raman spectroscopy of stored red blood cell concentrate within sealed transfusion blood bags

The Analyst ◽  
2018 ◽  
Vol 143 (24) ◽  
pp. 6006-6013 ◽  
Author(s):  
M. Z. Vardaki ◽  
C. G. Atkins ◽  
H. G. Schulze ◽  
D. V. Devine ◽  
K. Serrano ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Blood Transfusion ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Spectral Information ◽  
Stored Blood

Spectral information relevant to the quality of stored blood can be obtained in situ through sealed blood transfusion bags using a commercially available instrument.

scholarly journals Haematological and Biochemical Values of Immunosuppressed BALB/c NUDE and C57BL/6 SCID Mice

2018 ◽  
Vol 46 (1) ◽  
pp. 7
Author(s):  
Érika Almeida Praxedes ◽  
Muriel Magda Lustosa Pimentel ◽  
Fernanda Araujo Dos Santos ◽  
Denilsa Pires Fernandes ◽  
Brenda De Sousa Barbosa ◽  
...  
Keyword(s):  
Blood Cell ◽  
Reference Values ◽  
Red Blood Cell ◽  
Biochemical Parameters ◽  
Haemoglobin Concentration ◽  
Gene Mutations ◽  
Scid Mice ◽  
Blood Collection ◽  
Biochemical Tests ◽  
Cell Series

Background: The emergence of the NUDE and SCID immunosuppressed mice lineages generated knowledge on various mechanisms of lymphocyte maturation and human autoimmune diseases. Information on haematological and biochemical parameters of these lineages is still scarce, making it impossible to infer homeostasis by comparing data, or to detect genetic influences on the parameters for these species. Haematological and biochemical tests were carried out on Balb/c NUDE and C57BL/6 SCID mice of both sexes, aiming to analyse the presence of genetic influence on possible variations of such parameters and to verify reference values for both lineages.Materials, Methods & Results: One hundred and forty mice (Mus musculus) of the Balb/C NUDE and C57BL/6 SCID lineages were used in the present study. The animals were previously anesthetized, the blood collection procedure was performed by cardiac puncture and the samples were collected in the presence of heparin and intended for haematological and biochemical evaluation, under standardized conditions. The haematological evaluation consisted of red blood cell count, leukocyte counts, platelet counts, haematocrit, haemoglobin concentration, mean corpuscular volume (MCV), and mean corpuscular haemoglobin concentration (MCHC). The quantified biochemical parameters were: urea, creatinine, alanine aminotransaminase (ALT), aspartate aminotransaminase (AST) and alkaline phosphatase (ALP). While analysing the obtained data, it was possible to observe that only females presented divergences (P < 0.05) in the red blood cell series, in haemoglobin and in mean haemoglobin concentration (MCH). Regarding the analysis of the white blood cell series, females only presented differences (P < 0.05) in the leukocyte count. For males, there were variations (P < 0.05) in the counts of leukocytes, eosinophils, lymphocytes and neutrophils. Biochemical tests revealed significant variations (P < 0.05) in ALP and creatinine in females, and ALT, AST and ALP in males.Discussion: As a result of variations due to factors such as genetics, age, diet, sex and environmental conditions, it is of great importance that each research group establishes its own hematological and biochemical reference values for the mice strain used. In this way, possible variations and their causes can be determined, aiding in evaluations of homeostatic and pathological conditions for these animals, as well as their choice and analysis of results obtained in experimental procedures. Thus, the differences found only in females for parameters of the red blood cell series may be due to gene mutations responsible for haematopoiesis or the genetic background. Furthermore, in case of divergences found in the leukocyte parameters, females and males can present different immune responses due to their sex hormones, and females apparently have better performance in these responses. Regarding the other differences found in the leukogram parameters only in males, higher values in the NUDE lineage was attributed to possible stress situations increased by the genetic hormonal factors of the lineage. In addition, a correlation with anaesthetic administration is suggested regarding the variations found in the biochemical parameters, added to the stress generated at the muscular level. In conclusion, this study has presented pioneer values which enable expanding the still scarce knowledge database on immunosuppressed animals, aiding in future studies conducted with the Balb/c NUDE and C57BL/6 SCID lineages.

Using Raman spectroscopy to assess hemoglobin oxygenation in red blood cell concentrate: an objective proxy for morphological index to gauge the quality of stored blood?

The Analyst ◽  
2017 ◽  
Vol 142 (12) ◽  
pp. 2199-2210 ◽  
Author(s):  
Chad G. Atkins ◽  
H. Georg Schulze ◽  
Deborah Chen ◽  
Dana V. Devine ◽  
Michael W. Blades ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Morphological Index ◽  
Hemoglobin Oxygenation ◽  
Stored Blood

A relationship has been found between hemoglobin oxygenation of stored red blood cells (measured using Raman spectroscopy) and a morphological index.

scholarly journals Age- and sex-associated differences in hematology and biochemistry parameters of Dunkin Hartley guinea pigs (Cavia porcellus)

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0253794
Author(s):  
Alexa P. Spittler ◽  
Maryam F. Afzali ◽  
Sydney B. Bork ◽  
Lindsey H. Burton ◽  
Lauren B. Radakovich ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Red Blood Cell ◽  
Guinea Pigs ◽  
Biochemical Parameters ◽  
Hemoglobin Concentration ◽  
Reference Intervals ◽  
Blood Cell Count ◽  
Distribution Width ◽  
Age And Sex

The Dunkin Hartley is the most common guinea pig strain used in biomedical research, particularly for studies of asthma, allergy, infectious disease, reproduction, and osteoarthritis. Minimally invasive blood tests, such as complete blood counts and serum biochemistry profiles, are often collected for diagnostics and laboratory analyses. However, reference intervals for these assays have not yet been well-documented in this strain. The purpose of this study was to establish reference intervals for hematologic and biochemical parameters of Dunkin Hartley guinea pigs and determine age- and sex-related differences. Hematologic and biochemical parameters were retrospectively obtained from 145 male and 68 female guinea pigs between 2 and 15 months of age. All blood parameters were analyzed by a veterinary clinical pathology laboratory. Reference intervals were established according to the American Society for Veterinary Clinical Pathology guidelines. Age- and sex-related differences were determined using unpaired t-tests or nonparametric Mann-Whitney tests. Hematocrit, red blood cell distribution width, mean platelet volume, white blood cell count, heterophils, monocytes, eosinophils, glucose, blood urea nitrogen, creatinine, calcium, magnesium, total protein, albumin, globulin, cholesterol, aspartate aminotransferase, gamma glutamyl transferase, and bicarbonate increased with age. Mean corpuscular hemoglobin concentration, cellular hemoglobin concentration mean, platelets, lymphocytes, phosphorus, albumin/globulin ratio, alkaline phosphatase, anion gap, and calculated osmolality decreased with age. Males had higher hemoglobin, hematocrit, red blood cell count, mean corpuscular hemoglobin concentration, white blood cell count, heterophils, Foa-Kurloff cells, alanine aminotransferase, and bicarbonate and lower mean corpuscular volume, red blood cell distribution width, platelets, mean platelet volume, eosinophils, total protein, albumin, globulin, cholesterol, potassium, anion gap, calculated osmolality, and iron compared to females. Establishing age and sex differences in hematologic and biochemical parameters of Dunkin Hartley guinea pigs provides valuable insight into their physiology to better evaluate diagnostics and experimental results.

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