scholarly journals Stem cell secretome derived from human amniotic fluid affords neuroprotection in an ischemic model

2021 ◽  
Vol 7 (1) ◽  
pp. 18
Author(s):  
Liborio Stuppia ◽  
Chase Kingsbury
Keyword(s):  
Stem Cell ◽  
Amniotic Fluid ◽  
Human Amniotic Fluid

scholarly journals Human Amniotic Fluid Mesenchymal Stem Cells from Second- and Third-Trimester Amniocentesis: Differentiation Potential, Molecular Signature, and Proteome Analysis

2015 ◽  
Vol 2015 ◽  
pp. 1-15 ◽  
Author(s):  
Jurate Savickiene ◽  
Grazina Treigyte ◽  
Sandra Baronaite ◽  
Giedre Valiuliene ◽  
Algirdas Kaupinis ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Amniotic Fluid ◽  
Expression Patterns ◽  
Specific Gene ◽  
Specific Cell ◽  
Third Trimester ◽  
Human Amniotic Fluid ◽  
Differentiation Potential

Human amniotic fluid stem cells have become an attractive stem cell source for potential applications in regenerative medicine and tissue engineering. The aim of this study was to characterize amniotic fluid-derived mesenchymal stem cells (AF-MSCs) from second- and third-trimester of gestation. Using two-stage protocol, MSCs were successfully cultured and exhibited typical stem cell morphological, specific cell surface, and pluripotency markers characteristics. AF-MSCs differentiated into adipocytes, osteocytes, chondrocytes, myocytes, and neuronal cells, as determined by morphological changes, cell staining, and RT-qPCR showing the tissue-specific gene presence for differentiated cell lineages. Using SYNAPT G2 High Definition Mass Spectrometry technique approach, we performed for the first time the comparative proteomic analysis between undifferentiated AF-MSCs from late trimester of gestation and differentiated into myogenic, adipogenic, osteogenic, and neurogenic lineages. The analysis of the functional and expression patterns of 250 high abundance proteins selected from more than 1400 demonstrated the similar proteome of cultured and differentiated AF-MSCs but the unique changes in their expression profile during cell differentiation that may help the identification of key markers in differentiated cells. Our results provide evidence that human amniotic fluid of second- and third-trimester contains stem cells with multilineage potential and may be attractive source for clinical applications.

291 ISOLATION AND CHARACTERIZATION OF MESENCHYMAL STEM CELLS DERIVED FROM HUMAN AMNIOTIC FLUID

2011 ◽  
Vol 23 (1) ◽  
pp. 243 ◽  
Author(s):  
S.-A. Choi ◽  
J.-H. Lee ◽  
K.-J. Kim ◽  
E.-Y. Kim ◽  
K.-S. Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Amniotic Fluid ◽  
Adult Stem Cells ◽  
Embryonic Stem ◽  
Stem Cell Marker ◽  
Second Trimester ◽  
Cell Marker ◽  
Human Amniotic Fluid ◽  
Amniotic Fluid Stem Cells

Adult stem cells have the capacity to differentiate into several different cell types, although their differentiation potential is limited compared with that of embryonic stem cells. Thus, adult stem cells are regarded as an exciting source for new cell therapies. Recent observations also indicate that stem cells derived from second-trimester amniocentesis are pluripotent – capable of differentiating into multiple lineages, including representatives of all 3 embryonic germ layers. In addition, amniotic fluid stem cells can be used in the generation of disease- or patient-specific stem cells, and amniotic fluid stem cells could be an ideal source for autologous cell replacement therapy in the later life of the fetus. The aim of the present study was to investigate isolation and characterisation of human amniotic fluid-derived mesenchymal stem cells (hAFS). We successfully isolated and characterised hAFS. Amniotic fluid samples were collected in the second trimester (median gestational age: 16 weeks, range: 15–17 weeks) for prenatal diagnosis. Specimens (2 mL) were centrifuged and incubated in low-glucose DMEM supplemented with 10% FBS, 25 ng of basic fibroblast growth factor, and 10 ng of epidermal growth factor at 37°C with 5% CO2. Human amniotic fluid cell (passage 6) expression of stem cell specific markers OCT-4, SOX2, Rex1, FGF4, and NANOG was confirmed by RT-PCR. Flow cytometric analysis showed that hAFS (passage 10) were positive for CD44, CD29, CD146, STRO1, and CD90 but negative for CD19. Immunocytochemical analysis of hAFS (passage 11) also showed the expression of OCT-4, SSEA-1, CD44, CD29, CD146, STRO1, and CD90, but hAFS were negative for CD19 and CD14. In conclusion, according to the previous studies on other mammalians, hAFS are an appropriate source of pluripotent stem cells. Here, we demonstrated that hAFS have a high expression of stem cell specific marker, including embryonic stem cell marker and mesenchymal stem cell marker. Therefore, amniotic fluid may be a suitable alternative source of multipotent stem cells.

scholarly journals Comparative proteomic analysis of two distinct stem-cell populations from human amniotic fluid

2015 ◽  
Vol 11 (6) ◽  
pp. 1622-1632 ◽  
Author(s):  
Rita Romani ◽  
Francesca Fallarino ◽  
Irene Pirisinu ◽  
Mario Calvitti ◽  
Anna Caselli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Amniotic Fluid ◽  
Proteomic Analysis ◽  
Cell Populations ◽  
Human Amniotic Fluid ◽  
Comparative Proteomic Analysis ◽  
Stem Cell Populations

Characterization of two types of stem cells isolated from human amniotic fluid.

The human amniotic fluid stem cell secretome exerts cardio-active paracrine potential for myocardial repair and regeneration

Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S171
Author(s):  
A. Costa ◽  
C. Balbi ◽  
P.R. Garbati ◽  
K. Lodder ◽  
T. van Herwaarden ◽  
...  
Keyword(s):  
Stem Cell ◽  
Amniotic Fluid ◽  
Myocardial Repair ◽  
Human Amniotic Fluid ◽  
Amniotic Fluid Stem Cell

Enhancement of cell retention and functional benefits in myocardial infarction using human amniotic-fluid stem-cell bodies enriched with endogenous ECM

Biomaterials ◽  
2011 ◽  
Vol 32 (24) ◽  
pp. 5558-5567 ◽  
Author(s):  
Wen-Yu Lee ◽  
Hao-Ji Wei ◽  
Wei-Wen Lin ◽  
Yi-Chun Yeh ◽  
Shiaw-Min Hwang ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Stem Cell ◽  
Amniotic Fluid ◽  
Human Amniotic Fluid ◽  
Cell Retention ◽  
Amniotic Fluid Stem Cell

Exploiting the human amniotic fluid stem cell secretome for myocardial renewal

2020 ◽  
Vol 132 ◽  
pp. 106707
Author(s):  
A. Costa ◽  
C. Balbi ◽  
K. Lodder ◽  
P. Garbati ◽  
T. van Herwaarden ◽  
...  
Keyword(s):  
Stem Cell ◽  
Amniotic Fluid ◽  
Human Amniotic Fluid ◽  
Amniotic Fluid Stem Cell

scholarly journals Human Amniotic Fluid Stem Cell Preconditioning Improves Their Regenerative Potential

2012 ◽  
Vol 21 (11) ◽  
pp. 1911-1923 ◽  
Author(s):  
Cinzia Rota ◽  
Barbara Imberti ◽  
Michela Pozzobon ◽  
Martina Piccoli ◽  
Paolo De Coppi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Amniotic Fluid ◽  
Human Amniotic Fluid ◽  
Amniotic Fluid Stem Cell

scholarly journals Tuberin and PRAS40 are anti-apoptotic gatekeepers during early human amniotic fluid stem-cell differentiation

2011 ◽  
Vol 21 (5) ◽  
pp. 1049-1061 ◽  
Author(s):  
Christiane Fuchs ◽  
Margit Rosner ◽  
Helmut Dolznig ◽  
Mario Mikula ◽  
Nina Kramer ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Amniotic Fluid ◽  
Stem Cell Differentiation ◽  
Human Amniotic Fluid ◽  
Amniotic Fluid Stem Cell ◽  
Early Human
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