scholarly journals Evaluation and comparison of conjunctival swab polymerase chain reaction results in SARS-CoV-2 patients with and without ocular manifestations

2021 ◽  
Vol 69 (8) ◽  
pp. 2211
Author(s):  
Prempal Kaur ◽  
Gaurang Sehgal ◽  
Shailpreet ◽  
KD Singh ◽  
Bhavkaran Singh
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Ocular Manifestations ◽  
Conjunctival Swab ◽  
Polymerase Chain

scholarly journals OCULAR MANIFESTATIONS IN LABORATORY CONFIRMED COVID-19

2021 ◽  
Vol 5 (8) ◽  
Author(s):  
Shakti Krishan Rajguru ◽  
Udesh Rajpurohit
Keyword(s):  
Polymerase Chain Reaction ◽  
Rt Pcr ◽  
Ocular Pain ◽  
Chain Reaction ◽  
Hospital Unit ◽  
Ocular Manifestations ◽  
Ocular Symptoms ◽  
Eyelid Swelling ◽  
Polymerase Chain ◽  
Isolation Facility

Background: Scarce literature has been published thus far regarding ocular findings in the COVID-19. Methods: All patients were confirmed by SARS-CoV-2 real-time reverse transcriptase– polymerase chain reaction (RT-PCR) of nasopharynx or oropharynx swab specimens collected at an isolation facility, fever clinic, or hospital unit designated for suspected cases pending confirmation. Results: Overall, 25 patients (25.00%) had various ocular symptoms during COVID-19 infection. Among those with ocular symptoms, 19 patients (76.00%) presented with increased conjunctival discharge, including white mucoid (6 [24.00%]), thin watery (3 [12.00%]) and yellow-green purulent (11 [44.00%]) discharge, and with conjunctival congestion (2 [8.00%]). Other ocular manifestations contained ocular pain (3 [12.00%]), tearing (1 [4.00%]), and eyelid swelling (1 [4.00%]). Conclusion: We concluded that common ocular manifestations were conjunctival discharge, eye rubbing, and conjunctival congestion. Keywords: COVID-19, Ocular,  Eye.

scholarly journals The Prevalence of ocular manifestations and ocular samples polymerase chain reaction positivity in patients with COVID 19 - a systematic review and meta-analysis

2020 ◽  
Author(s):  
Soumen Sadhu ◽  
Sushmitha Arcot Dandapani ◽  
Deepmala Mazumdar ◽  
Sangeetha Srinivasan ◽  
Jyotirmay Biswas
Keyword(s):  
Systematic Review ◽  
Polymerase Chain Reaction ◽  
Case Reports ◽  
Meta Analysis ◽  
Initial Symptom ◽  
Chain Reaction ◽  
Ocular Manifestations ◽  
Polymerase Chain Reaction Positivity ◽  
Pooled Prevalence ◽  
Polymerase Chain

AbstractObjectiveTo estimate the prevalence of ocular manifestations and ocular samples polymerase chain reaction (PCR) positivity among COVID-19 patients.MethodsA systematic literature review was performed using search engines (PubMed, Google Scholar, Medrixv and BioRixv) with keywords “SARS-CoV-2”, “novel coronavirus”, “COVID-19”, “ocular manifestations”, “conjunctival congestion”, “Ocular detection”, “Polymerase chain reaction”, and “conjunctivitis”. The measure of heterogeneity was evaluated with the I2 statistic. The pooled proportion of patients presenting with symptoms and ocular samples PCR positivity was estimated.ResultsA total of 20 studies (14 studies and 6 case-reports) were included in the systematic review and 14 studies were included in the meta-analysis. The pooled prevalence of conjunctivitis was 5.17% (95% CI: 2.90-8.04). Conjunctivitis was reported as an initial symptom of the disease in 0.858 % (95% CI: 0.31-1.67). Common associated features include itching, chemosis, epiphora. Seven patients (29 %) with conjunctivitis showed positive results in ocular samples, whereas 13 patients (54%) showed positive only in their nasopharyngeal samples (NPs) or sputum samples and 4 patients (16 %) were negative for both NPs and Sputum as well as ocular samples. The pooled prevalence of ocular PCR positivity was 2.90 % (95% CI: 1.77 – 4.46) vs. NPs 89.8% (95% CI: 78.80-79.0).ConclusionThe prevalence of conjunctivitis and ocular samples PCR positivity among COVID-19 patients was low indicating that the eye is a less affected organ. However, conjunctivitis may present as the first symptom of the disease making the patient seek medical care at the earliest.SynopsisViral conjunctivitis was the only symptom reported. The prevalence of conjunctivitis and ocular samples polymerase chain reaction positivity among COVID-19 patients was low indicating the eye is a less effected organ.

scholarly journals Polymerase chain reaction using conjunctival swab samples for detecting Leishmania DNA in dogs

2021 ◽  
Vol 30 (3) ◽  
Author(s):  
Karen Araújo Magalhães ◽  
Kamily Fagundes Pussi ◽  
Hélton Krisman de Araújo ◽  
Silvia Barbosa do Carmo ◽  
Elisabete Friozi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Canine Leishmaniasis ◽  
Chain Reaction ◽  
Promising Alternative ◽  
Non Invasive ◽  
Significant Difference ◽  
Conjunctival Swab ◽  
Important Host ◽  
Polymerase Chain ◽  
Ocular Changes

Abstract The dog is the main domestic reservoir of Leishmania and font of infection for the vector, constituting an important host for the transmission of the parasite to humans. Non-invasive collection of swab samples for leishmaniasis diagnosis has been a promising alternative. This study analyzed the positivity of polymerase chain reaction (PCR) for the diagnosis of canine leishmaniasis in conjunctiva samples. DNA extraction was performed using SDS 20% and PCR was performed using 13A/13B primers that amplify 120-bp of Leishmania kDNA. Of the 77 dogs analyzed, 50 (64.93%) had ocular changes: 25 (32.47%) dogs had periocular lesion, 41 (53.25%) dogs had purulent eye discharge, and 17 (22.08%) dogs had both signals. PCR was positive in 35 dogs (45.45%), and there was no significant difference between dogs with and without ocular signals (p=0.4074). PCR positivity was significant higher in dogs without periocular injury (p=0.0018). Conjunctive PCR, a less invasive, fast, and painless collection technique, is indicated to complement the diagnosis, especially in dogs without periocular injury, independent of the presence of purulent eye discharge.

scholarly journals Ocular involvment of coronavirus disease (COVID-19): A systematic review of conjunctival swab results

2020 ◽  
Vol 3 (2) ◽  
pp. 118
Author(s):  
Indra Tri Mahayana ◽  
Natalia Christina Angsana ◽  
Muhammad Zhafran Ayyasy ◽  
Anastasya Sondang Hutajulu ◽  
Suhardjo Suhardjo
Keyword(s):  
Systematic Review ◽  
Ocular Surface ◽  
Scientific Literature ◽  
Inclusion Criteria ◽  
Chain Reaction ◽  
Research Papers ◽  
Ocular Manifestations ◽  
Pubmed Database ◽  
Conjunctival Swab ◽  
Polymerase Chain

Coronavirus disease (COVID-19) is a pandemic disease which is caused by the latest discovered coronavirus. Conjunctivitis is allegedly the first presentation of COVID-19 since it can spread by aerosol contact with the conjunctiva. The present study aimed to systematically review the employment of conjunctival swab with Real-time Polymerase Chain Reaction in detecting SARS-Cov-2. The research is a systematic review of the published scientific literature on findings of conjunctival swab of COVID-19 from PUBMED database and other additional sources (i.e: Google Scholar). The search method was done using “COVID-19 OR coronavirus OR SARS-COV2, AND conjunctivitis, AND ocular manifestations, AND conjunctival swab” as keywords. Inclusion criteria were any papers that related to the entered keywords and have conjunctival swab as a reported outcome. Letters, reviews, and editorials describing other studies reporting COVID-19 and conjunctival swab were excluded. Only four research papers were found and included in the literature review. From the four current research papers, positive SARS-CoV-2 results were yielded from 0-5.26% of conjunctival swab specimens. In conclusion, although the presence of the SARS-CoV-2 virus on the ocular surface remains unclear, the prevention of infection transmission through an ocular surface is mandatory by wearing goggles (or shield), mask (N-95 recommended) and gown.

Using Conjunctival Swab with Polymerase Chain Reaction to Aid Diagnosis of Cytomegalovirus Retinitis in AIDS Patients

2000 ◽  
Vol 214 (2) ◽  
pp. 126-130 ◽  
Author(s):  
Jorn-Hon Liu ◽  
Wen-Ming Hsu ◽  
Wing-Wai Wong ◽  
Jaang-Jiun Wang ◽  
Wu-Tse Liu ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cytomegalovirus Retinitis ◽  
Chain Reaction ◽  
Aids Patients ◽  
Conjunctival Swab ◽  
Polymerase Chain

Detection of Human Cytomegalovirus Retinitis and Monitoring of Ganciclovir Treatment Using Conjunctival Swab with Polymerase Chain Reaction in AIDS Patients

2000 ◽  
Vol 11 (2) ◽  
pp. 85-91
Author(s):  
S H Chiou ◽  
J H Liu ◽  
W W Wong ◽  
Y J Chan ◽  
Y C Chang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Cytomegalovirus ◽  
Predictive Value ◽  
Cytomegalovirus Retinitis ◽  
Chain Reaction ◽  
Aids Patients ◽  
Conjunctival Swab ◽  
Patients At Risk ◽  
Polymerase Chain ◽  
Sensitivity Specificity

This report studies the accuracy of conjunctival swab polymerase chain reaction (CS-PCR) for the diagnosis of human cytomegalovirus retinitis (HCMV) in AIDS patients. PCR and virus culture were used for the detection of HCMV in conjunctival swab, serum, and urine specimens from 38 AIDS patients between April 1996 and April 1998. The clinical utility of the identification of HCMV retinitis by these 6 different methods was demonstrated by their prediction power to estimate AIDS patients at risk of contracting HCMV retinitis. The sensitivity, specificity, positive predictive value, and negative predictive value of CS-PCR for the detection of HCMV retinitis were 91.5%, 80.9%, 60.8%, and 92.7%, respectively; for serum PCR were 74.3%, 81.7%, 57.2%, and 90.3%; for urine PCR were 100%, 17.3%, 20.4%, and 100%; for conjunctival swab culture were 22.7%, 100%, 100%, and 86%; for serum culture were 27.3%, 98.1%, 75%, and 86.4%; and for urine culture were 90.9%, 44.2%, 25.6%, and 95.8%.

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